Investigation the level of IL-10 and IFN-γ in mice infected with Entamoeba histolytica and treated with earthworm powder
DOI:
https://doi.org/10.23851/mjs.v30i1.490Keywords:
Earthworm powder, Entamoeba histolytica, IFN-γ, IL-10Abstract
This study was carried out to detect the effect of earthworm powder on the Entamoeba histolytica in white mice and to measure the serum of two cytokines (IFN-γ and IL-10) of infected and treated mice with earthworm powder in comparing with metronidazole drug after 3, 7 and 9 days. Result showed that the infected mice which treated with earthworm powder in a concentration of 200 mg/ml for 10 days the level of IFN-γ in serum was (170.67, 150.33 and 145.67 pg/ml) respectively compared with control group, in the earthworm powder group (none infected) level of IFN-γ after 3, 7, 9 days was (105.50, 121.50, 127.00 pg/ml) respectively, also in the metronidazole treatment group IFN-γ level after 3, 7, 9 days was (188.33, 263.50, 253.33 pg/ml) respectively compared to control group. In the treatment earthworm powder group level IL-10 after 3, 7, 9 days was (108.67, 105.67 and 103.17 pg/ml) respectively compared with control group, but in the earthworm powder group (none infected) level IL-10 after 3, 7, 9 days was (95.67, 95.83, 95.67 pg/ml) respectively, also in the metronidazole treatment group IL-10 level after 3, 7, 9 days was (109.83, 107.67, 103.33 pg/ml) respectively compared with control groups.Downloads
References
Al-Hadraawy, S.; Al-qraby, M. & Al-Shebly, F. 2015. Study of Some Parameter in Men Infected with Entamoeba histolytica in Al-Najaf Al-Ashraf Province. Journal of Babylon University/Pure and Applied Sciences., 2:23.
Andleeb, S.; Ejaz, M.; Awan, U.; Ali, S.; Kiyani, A.; Shafique, I & Zafar, A. 2016. In vitro screening of mucus and solvent extracts of Eisenia fotida against human bacterial and fungal pathogens. Pak.J.Pharm.Sci., 29(3) :969-977.
Anjana, J.C.; Sruthy, P. B.; Rathinamala, J.& Jayashree, S. 2013. Evaluation of earthworm powder (Eudrilus eugeniae) and its application in cotton crepe badage. International Journal of Bioassays., 2 (9):1250-1255.
Bansal, D.; Sehgal, R.; Chawla, Y.; Malla, N. & Mahajan R C. 2006. Multidrug resistance in amoebiasis patient. Indian J Med Res, 124: 189-194.
Bhorgin, A.J. & Uma, K. 2014. Antimicrobial activity of Earthworm Powder (Lampito mauritii). International Journal of Current Microbiology and Applied Sciences., 3(1):437-443.
Caler, E. & Lorenzi, H. 2010. "Entamoeba histolytica: Genome Status and Web Resources". Anaerobic Parasitic Protozoa: Genomics and Molecular Biology.
Coppi, A.; Cabinian, M.; Mirelman, D. & Sinnis, P. 2006. Antimicrobial activity of Allicin abiologically active compound from garlic cloves Microb. Agent.chem.,50(5): 1731-1737.
Eisenstein, B. I. & Schaechter, M. 2007. "DNA and Chromosome Mechanics". In Schaechter, M.; Engleberg, N. C.; DiRita, V. J.; Schaechter's Mechanisms of Microbial Disease. Hagerstown, MD: Lippincott Williams & Wilkins. p. 28.
Fang, Y. S. 1999. Eisenia fetida fibrinolytic enzymes research VIII: determination of fibrinolytic enzymes active components. Heilong Jiang Journal of Medicine., 12: 263-264.
Guo, X.; Barroso, L.; Becker, S.; Lyerly, D.; Vedvick, T.; Reed, S. and Petri, W. 2009. Protection against Intestinal Amebiasis by a Recombinant Vaccine Is Transferable by T Cells and Mediated by Gamma Interferon. Infect., 77(9): 3909-3918.
Mathur, A.; Verma, S.K.; Bhat, R.; Singh, S.K.; Prakash, A.; Prasad G. & Dua, V.K. 2010. Antimicrobial Activity of Earthworm Extracts., J. Chem. Pharm. Res., 2(4): 364-370.
Mohammed, S. T.; Faleh, N. & Salman, S, D. 2016. Investigation the Role of Earthworm powder as anti -leishmanial Activity. Australian Journal of Basic and Applied Sciences, 10(15): 299-305.
Mortimer, L. & Chadee, K. 2010. The immunopathogenesis of Entamoeba histolytica. Exp. Parasitol., 126 (3): 336-380.
Murry, P. R.; Rosenthal, K. S. & Pfaller, M. A. 2013. Medical Microbiology. 27th ed. Saunders, an imprint of Elsevier Inc. pp:745-748.
Omar, H.M.; Ibraheim, Z.Z.; EL-Shimy N.A. & Ali, R.S. 2012. Anti- inflammatory, antipyretic and antioxidant activities of the earthworm extract. Journal of Biology and Earth Sciences, 2(1).
Prakash, M & G. Gunasekaran, 2011. Antibacterial activity of the indigenous earthworms Lampito mauritii (Kinberg) and Perionyx xcavates (Perrier). J Altern Complement Med., 17(2): 167-170.
Roberts- Thompson IC; Stevens DP, Mahmoud AAF, Warren K. 1976. Giardiasis in the mouse: an animal model. Gastroentrology, 71:57-61.
Sannella, A.; Gradoni, L.; Persichini, T.; Ongini, E.; Venturini, G. & Colasanti. M. 2003. Intracellular release of Nitric Oxide by NCX 972, a No-releasing Metronidazole, enhances in vitro killing of E. histolytica. Anti microb. Agents Chemo, 47(7):2303-2306.
SAS. 2012. Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA.
Shannon, N.; Moonah, N.; and William, A. 2013. Host Immune Response to Intestinal Amebiasis. PLOS Pathoge., (9): 80- 85.
Singh, U.P., Prithiviraj, B.; Sharma, B.K., Singh, M., & Ray, A.B., 2001. Role of garlic Allium sativum in human and plant diseases. Ind. J. exp. Bio., 39(4): 310-322.
Sonja, L. & Carl, E. N. 2010. Metronidazole is still the drug of choice for treatment of anaerobic infections. Clin. Infect. Dis., 50, 1: 23.
Swiderski, F.; Dabrowski, M.; Rusaczone, K. A. & Waszkiewicz, B. 2007. Bio active substances of garlic and their role in dietoprophylaxis and dieto therapy. Rocz. Panstw. Zaki. Hig., 58(1) :41-60.
Tachibana, H.; Matsumoto, N.; Tsukamoto, H.& Yoshihara, E. 2004. Improved affinity of a human anti- E. histolytica GalGalNAc Lectin Fab fragment by a single amino acid modification of the light chain. Clin. Diagno. Lab. Immunol., 11(6):1085-1088.
Tanyuksel, M.; Yilmaz, H.; Ulukanligil, M.; Araz, E.; Cicek, M.; Koru, O.; Tas, Z. & Petri, W.A. 2005. Comparison of two methods (microscopy and enzyme-linked immunosorbent assay) for the diagnosis of amoebiasis. Exp. Parasitol., 110:322-326.
Von Brand, T., C., Rees, R., Jacobs, L. and Reardon, L. V. 1943. Studies on reducing substances and gas formation in cultures of Endamoeba histolytica and a single species of symbiotic bacterium. Am. J. Hyg , 37, pp:310-319.
Wain, A.M. 1998. Metronidazole vaginal gel 0.75% (Metrogel- vaginal): a brief review. Infect. Dis. Obstet. Gynecol, 6, pp: 3–7.
Yegnanarayan, R.; Sethi, P. P.; Bajhan, P.A.; Pulandiran, K. & Ismail, S. A. 1987. Anti-inflammatory activity of total earthworm extracts in rats India. J. Pharmacol., 19:221-222.
Yu, S.; Yu, Y. & NiLun T. 2005. Capsaicin biosyn thesis in water stressed hot pepper fruits. Bot. Bull. Acad. Sin., (46):35-42.
Yu, Z. X. 1998. Analysis of earthworm fibrinolytic enzymes composition. China Journal of Biochemistry and Molecular Biology. 14: 407-411.
Zakaria, Z.; Saleh, N.; Mohamed. A.; Anas, N.G. & Idris, S. 2012. Optimization of Protein Content in Earthworm-based Fish Feed Formulation for Catfish (Clarius gariepinus). Sains Malaysian., 41(9):1071-1077.
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