Unveiling the High Prevalence of Antibiotic Resistance and Quorum Sensing Genes in Uropathogenic Escherichia coli


  • Hanan M. Abbas Department of Biology, Faculty of Sciences, Sfax University, Sfax, TUNISIA. https://orcid.org/0009-0007-8838-2136
  • Radhouane Gdoura Research Laboratory of Toxicology Microbiology Environmental and Health, Sfax University, Faculty of Sciences, BP 1171, 3000, TUNISIA. https://orcid.org/0000-0003-1140-3780
  • Mohammed F. Al-Marjani Department of Biology, College of Science, Al-Mustansiriyah University, 10052 Baghdad, IRAQ.




motA, E. coli, Quorum Sensing, luxS


Escherichia coli is considered one of the uropathogenic bacteria with different infection symptoms representing mild illness to acute sepsis. This study aims to detect E. coli in patients with urinary infection and investigate quorum sensing genes (lux S and motA) in multi-drug resistant isolates of E. coli.  200 urine samples were collected from patients with urinary tract infections from several hospitals in Baghdad. The antibiotics sensitivity test showed high resistance of isolates for Ampicillin (100%), Cefazolin (97%), Trimethoprim/ Sulfamethoxazole (83%), Ceftriaxone (77%), Ceftazidime and Ciprofloxacin (70% each of them), and moderate resistance of isolates for Levofloxacin (50%), Gentamicin (47%), Cefepime (40%), while low resistance Piperacillin/ Tazobactam (33%), Cefoxitin (30%), Nitrofurantoin (17%), Imipenem (10%), Ertapenem and Amikacin (7% each of them), and Tigecycline (3%). The results showed an increase percentage of infection in females was 30% in the ages 30-44 years, whereas in ages 15-29 and more than 45 years was 17%.  There was a high percentage (57.11%) of resistant isolates in females which are ages 30-44 years. While the ages more than 45 years were 66.4% and ages 15-29 were 34%. While, in males, the percentage was high in ages more than 45 years (35.25%) followed by age groups 30-44 years (31.5%) and 15-29 years (31%).  The prevalence percentage for luxS and motA genes in E. coli was 100%. In conclusion, E. coli isolates were multi-drug resistant due to all isolates had quorum sensing genes. Moreover, uropathogenic of E. coli in females was more frequent than in males due to the resistance of bacteria to antibiotics.





Download data is not yet available.


J. Bien, O. Sokolova, and P. Bozko, 'Role of uropathogenic Escherichia coli virulence factors in development of urinary tract infection and kidney damage', International journal of nephrology, vol. 2012, 2012.

CrossRef | PubMed

M. Gharbi et al., 'Antibiotic management of urinary tract infection in elderly patients in primary care and its association with bloodstream infections and all cause mortality: population based cohort study', bmj, vol. 364, 2019.

CrossRef | PubMed

I S. E. Geerlings, 'Clinical presentations and epidemiology of urinary tract infections', Microbiology spectrum, vol. 4, no. 5, pp. 4-5, 2016.

CrossRef | PubMed

G. A. Ouno et al., 'Isolation, identification and characterization of urinary tract infectious bacteria and the effect of different antibiotics', 2013.

K. S. P. Cabuhat and L. S. Moron-Espiritu, 'Quorum Sensing Orchestrates Antibiotic Drug Resistance, Biofilm Formation, and Motility in Escherichia coli and Quorum Quenching Activities of Plant-derived Natural Products: A Review.', Journal of Pure & Applied Microbiology, vol. 16, no. 3, 2022.


R. Mirghani et al., 'Biofilms: Formation, drug resistance and alternatives to conventional approaches', AIMS microbiology, vol. 8, no. 3, p. 239, 2022.

CrossRef | PubMed

C. Solano, M. Echeverz, and I. Lasa, 'Biofilm dispersion and quorum sensing', Current opinion in microbiology, vol. 18, pp. 96-104, 2014.

CrossRef | PubMed

R. V. Sionov and D. Steinberg, 'Targeting the holy triangle of quorum sensing, biofilm formation, and antibiotic resistance in pathogenic bacteria', Microorganisms, vol. 10, no. 6, p. 1239, 2022.

CrossRef | PubMed

S. I. Aizawa, 'Chapter 7- Flagella', in Molecular Medical Microbiology (Second Edition), Y.-W. Tang, M. Sussman, D. Liu, I. Poxton, and J. Schwartzman, Eds., Boston: Academic Press, 2015, pp. 125-146.

R. Yamaji et al., 'A population-based surveillance study of shared genotypes of Escherichia coli isolates from retail meat and suspected cases of urinary tract infections', Msphere, vol. 3, no. 4, pp. 10-1128, 2018.

CrossRef | PubMed

C. Zagaglia, M. G. Ammendolia, L. Maurizi, M. Nicoletti, and C. Longhi, 'Urinary tract infections caused by uropathogenic Escherichia coli strains-new strategies for an old pathogen', Microorganisms, vol. 10, no. 7, p. 1425, 2022.

CrossRef | PubMed

T. Shah, P. Preethishree, and P. V. Ashwini, 'Bacterial Profile of Urinary Tract Infections: Evaluation of Biofilm Formation and Antibiotic Resistance Pattern of Uropathogenic Escherichia coli. J Pure Appl Microbiol. 2020; 14 (4): 2577-2584.


F. Al-Gasha'a, S. Al-Baker, J. Obiad, and F. Alrobiai, 'Prevalence of urinary tract infections and associated risk factors among patients attending medical city hospital in Baghdad city, Iraq', Am J Infect Dis, vol. 16, no. 2, pp. 77-84, 2020.


K. K. Ghaima, Z. S. Khalaf, A. A. Abdulhassan, and N. Y. Salman, 'Prevalence and antibiotic resistance of bacteria isolated from urinary tract infections of pregnant women in Baghdad hospitals', Biomedical and Pharmacology Journal, vol. 11, no. 4, pp. 1989-1994, 2018.


M. Odoki et al., 'Prevalence of bacterial urinary tract infections and associated factors among patients attending hospitals in Bushenyi district, Uganda', International journal of microbiology, vol. 2019, 2019.

CrossRef | PubMed

A. T. Signing, W. J. T. Marbou, V. P. Beng, and V. Kuete, 'Antibiotic resistance profile of uropathogenic bacteria in diabetic patients at the Bafoussam Regional Hospital, West Cameroon Region', Cureus, vol. 12, no. 7, 2020.

CrossRef | PubMed

J. Ayatollahi, F. Fotouhi, Z. Akhondimeybodi, M. Hamidfar, and S. Hossein, 'Determination of antibiotic resistance of E. coli isolated from urine culture samples', The Journal of Medical Research, vol. 8, no. 5, pp. 162-164, 2022.


J. Gu, X. Chen, Z. Yang, Y. Bai, and X. Zhang, 'Gender differences in the microbial spectrum and antibiotic sensitivity of uropathogens isolated from patients with urinary stones', Journal of Clinical Laboratory Analysis, vol. 36, no. 1, p. e24155, 2022.

CrossRef | PubMed

V. M. Luna-Pineda et al., 'Features of urinary Escherichia coli isolated from children with complicated and uncomplicated urinary tract infections in Mexico', PloS one, vol. 13, no. 10, p. e0204934, 2018.

CrossRef | PubMed

V. Ballén, V. Cepas, C. Ratia, Y. Gabasa, and S. M. Soto, 'Clinical Escherichia coli: from biofilm formation to new antibiofilm strategies', Microorganisms, vol. 10, no. 6, p. 1103, 2022.

CrossRef | PubMed

X. P. Chen et al., 'Biofilm Formation Plays a Role in the Formation of Multidrug-Resistant Escherichia coli Toward Nutrients in Microcosm Experiments', Frontiers in Microbiology, vol. 9, 2018

CrossRef | PubMed


Key Dates











Original Article

How to Cite

H. M. Abbas, R. Gdoura, and M. F. Al-Marjani, “Unveiling the High Prevalence of Antibiotic Resistance and Quorum Sensing Genes in Uropathogenic Escherichia coli”, Al-Mustansiriyah Journal of Science, vol. 35, no. 1, pp. 38–43, Mar. 2024, doi: 10.23851/mjs.v35i1.1429.

Similar Articles

1-10 of 38

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)