Bacterial Filaments Induced by Antibiotic Minimal Inhibitory Concentrations in Persister Cells
DOI:
https://doi.org/10.23851/mjs.v35i2.1415Keywords:
E. coli, persister, filaments, ciprofloxacin, uspA geneAbstract
Background: The ability of minor subpopulations among clonal populations to survive antibiotics is referred to as bacterial persistence. It is believed that persisters come from latent cells, where antibiotic target areas are less active and incapable of being affected. Objective: 112 clinical Escherichia coli isolates were acquired out of diverse medical samples and genetically identified using the uspA gene, which is part of the housekeeping genes. Methods: The examination of persister cells was carried out by subjecting isolates of E. coli in the exponential phase with high dose of ciprofloxacin (20 fold MIC) and calculating the surviving persister cells using CFU (colony forming units) counts. The detection and measurement of bacterial filament production was done using scanning electron and light microscopy. Results: Results showed that the bacterial filament cells kept on lengthen but cease to divide (no septa formation) at sub-minimal inhibitory doses of ciprofloxacin. Persistent isolates were shown to exhibit a wide range of form and size variations, with cells up to 4.5 times longer than usual. Conclusions: The results showed the importance of antibiotic stress on persisted cells that result in the production of filaments as a means of survival and the need to examine these rare phenotypic variations. These occurrences may be the beginning of the spread of bacterial resistance.
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E. M. Darby, E. Trampari, P. Siasat, M. S. Gaya, I. Alav, M. A. Webber, and J. M. Blair, "Molecular mechanisms of antibiotic resistance revisited," Nature Reviews Microbiology, vol. 21, no. 5, pp. 280-295, 2023.
J. E. Sulaiman and H. Lam, "Evolution of bacterial tolerance under antibiotic treatment and its implications on the development of resistance," Frontiers in Microbiology, vol. 12, 2021.
E. Bakkeren, M. Diard, and W.-D. Hardt, "Evolutionary causes and consequences of bacterial antibiotic persistence," Nature Reviews Microbiology, 2020.
S. Jung, C.-M. Ryu, and J.-S. Kim, "Bacterial persistence: Fundamentals and clinical importance," Journal of Microbiology, vol. 57, no. 10, pp. 829-835, 2019.
S. N. Aziz, M. F. Al Marjani, A. M. Rheima, and I. M. S. Al Kadmy, "Antibacterial, antibiofilm, and antipersister cells formation of green synthesis silver nanoparticles and graphene nanosheets against klebsiella pneumoniae,"Reviews in Medical Microbiology, vol. 33, no. 1, pp. 56-63, 2021.
J. Bigger, "Treatment of staphyloeoeeal infections with penicillin by intermittent sterilisation," Lancet, pp. 497-500, 1944.
N. Q. Balaban, S. Helaine, K. Lewis, M. Ackermann, B. Aldridge, D. I. Andersson, and A. Zinkernagel, "Definitions and guidelines for research on antibiotic persistence," Nature Reviews Microbiology, vol. 17, no. 7, pp. 441-448, 2019.
T. K. Wood, S. J. Knabel, and B. W. Kwan, "Bacterial persister cell formation and dormancy," Applied and Environmental Microbiology, vol. 79, no. 23, pp. 7116-7121, 2013.
J. Bos, Q. Zhang, S. Vyawahare, E. Rogers, S. M. Rosenberg, and R. H. Austin, "Emergence of antibiotic resistance from multinucleated bacterial filaments," Proceedings of the National Academy of Sciences, vol. 112, no. 1, pp. 178-183, 2014.
D. C. Karasz, A. I. Weaver, D. H. Buckley, and R. C. Wilhelm, "Conditional filamentation as an adaptive trait of bacteria and its ecological significance in soils," Environmental Microbiology, vol. 24, no. 1, pp. 1-17, 2021.
W. C. Russell, C. Newman, and D. L. Williamson, "A simple cytochemical technique for demonstration of dna in cells infected with mycoplasmas and viruses," vol. 253, no. 5491, pp. 461-462, 1975.
R. R. Stackhouse, N. G. Faith, C. W. Kaspar, C. J. Czuprynski, and A. E. Wong, "Survival and virulence of salmonella enterica serovar enteritidis filaments induced by reduced water activity," vol. 78, no. 7, pp. 2213-2220, 2012.
T. T. Cushnie, N. H. O'Driscoll, and A. J. Lamb, "Morphological and ultrastructural changes in bacterial cells as an indicator of antibacterial mechanism of action," Cellular and Molecular Life Sciences, vol. 73, no. 23, pp. 4471-4492, 2016.
J. Chen and M. W. Griffiths, "Pcr differentiation of escherichia coli from other gram-negative bacteria using primers derived from the nucleotide sequences flanking the gene encoding the universal stress protein," Letters in Applied Microbiology, vol. 27, no. 6, pp. 369-371, 1998.
E. S. Chung, Y. M. Wi, and K. S. Ko, "Variation in formation of persister cells against colistin in acinetobacter baumannii isolates and its relationship with treatment failure," Journal of Antimicrobial Chemotherapy, vol. 72, no. 7, pp. 2133-2135, 2017.
J. Buijs, A. S. M. Dofferhoff, J. W. Mouton, J. H. T. Wagenvoort, and J. W. M. van der Meer, "Concentrationdependency of β-lactam-induced filament formation in gram-negative bacteria," Clinical Microbiology and Infection,vol. 14, no. 4, pp. 344-349, 2008.
H. Ren, X. He, X. Zou, G. Wang, S. Li, and Y. Wu, "Gradual increase in antibiotic concentration affects persistence ofklebsiella pneumoniae," Journal of Antimicrobial Chemotherapy, 2015
A. Brauner, O. Fridman, O. Gefen, and N. Q. Balaban, "Distinguishing between resistance, tolerance and persistence to antibiotic treatment," Nature Reviews Microbiology, vol. 14, no. 5, pp. 320-330, 2016.
F. Goormaghtigh and L. Van Melderen, "Single-cell imaging and characterization of escherichia coli persister cells to ofloxacin in exponential cultures," Science Advances, vol. 5, no. 6, 2019.
M. Li, Y. He, J. Sun, J. Li, J. Bai, and C. Zhang, "Chronic exposure to an environmentally relevant triclosan concentration induces persistent triclosan resistance but reversible antibiotic tolerance in escherichia coli," Environmental Science & Technology, vol. 53, no. 6, pp. 3277-3286, 2019.
H. Li, X. Zhou, Y. Huang, B. Liao, L. Cheng, and B. Ren, "Corrigendum: Reactive oxygen species in pathogen clearance: The killing mechanisms, the adaption response, and the side effects," Frontiers in Microbiology, vol. 12, 2021.
J. E. Sulaiman and H. Lam, "Proteomic study of the survival and resuscitation mechanisms of filamentous persisters in an evolved escherichia coli population from cyclic ampicillin treatment," mSystems, vol. 5, no. 4, 2020.
M. D. Chengalroyen et al., "Dna-dependent binding of nargenicin to dnae1 inhibits replication in mycobacterium tuberculosis," ACS Infectious Diseases, vol. 8, no. 3, pp. 612-625, 2022.
D. Wojnicz, M. Kłak, R. Adamski, and S. Jankowski, "Influence of subinhibitory concentrations of amikacin and ciprofloxacin on morphology and adherence ability of uropathogenic strains," Folia Microbiologica, vol. 52, no. 4, pp. 429-436, 2007.
B. H. Abdulwhab, "E. coli response under chemical stress: An experience with amikacin, gentamicin and ciprofloxacin," Al-Mustansiriyah Journal of Science, vol. 24, no. 4, 2013.
L. Wang et al., "Filamentation initiated by cas2 and its association with the acquisition process in cells," vol. 11, no. 3, 2019.
J. Joseph, S. Sharma, and V. P. Dave, "Filamentous gram-negative bacteria masquerading as actinomycetes in infectious endophthalmitis: A review of three cases," Journal of Ophthalmic Inflammation and Infection, vol. 8, no. 1, 2018.
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Copyright (c) 2024 Haneen N. Mohammed, Sawsan H. Authman, Mohammed F. AL Marjani, Shivanthi Samarasinghe
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