The Inhibition Potential of Oregano (Origanum Vulgare) Extract Against Citrobacter freundii Invitro and Invivo
Keywords:C. freundii, Oregano, oxidative stress, MDA, Glutathione
AbstractThe study was designed to demonstrate the antimicrobial and antioxidant effects of Oregano against C. freundii. The study consist of two parts, the first part (invitro) including study the sensitivity of C. freundii against safflower extract and antibiotics. where, the results showed very high inhibition efficacy of extract, which reached to 30 mm, while Neomycin inhibition zone reach to 14mm, Novobiocin inhibition zone reach to 9mm and Tetracycline inhibition zone reach to 9mm In the second part (in vivo) used 24 albino rats and divide to six groups (each group consist 4 rats). control group, group injected with (1x105 CFU/ml/25mg) bacteria, group injected with (1x105 CFU/ml/25mg) bacteria and treated with neomycin, group injected with (1x105 CFU/ml/25mg) bacteria and treated with novobiocin, group injected with (1x105 CFU/ml/25mg) bacteria and treated with tetramycine, group injected with (1x105 CFU/ml/25mg) bacteria with extract. The MDA levels in infected group and treated groups with antibiotics increased and GSH levels in infected group and treated groups with antibiotics show decreased (P < 0.05) compared control rats. In treated group with extract, MDA and GSH levels show on-significant change compare with control rats. It was concluded from present study that the Oregano has an antimicrobial and antioxidant activity against C. freundii.
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