Association of Housing Location and Sex with Cultivable Gut Microorganisms in Iraqi Infants
DOI:
https://doi.org/10.23851/mjs.v37i3.1916Keywords:
Infants, Intestinal flora, Infant sex, Housing location, Microbiome diversityAbstract
Background: The infant gut microbiota is essential for immune function, neurobehavioral development, and overall health. Therefore, studying how infants’ intestinal flora develops and what influences it is important. Early gut flora is affected by various factors, including internal ones such as maternal and infant-related aspects, and external environmental fac-tors like geographic location. Objective: To determine the diversity of the gut microbiota of Iraqi infants under two years of age by gender and by housing location (urban and rural). Methods: We collected 248 fecal specimens from infants under two years; 291 microbial isolates were identified by traditional methods (Gram staining, microscopy, biochemical tests), and bacterial growth was achieved using multiple culture media; the definitive diagnosis was then performed by sequence analysis. Results: The study shows a diverse intestinal microbiota, with fourteen species identified for the first time in Iraqi infants as newly identified strains in this community. Significant differences between urban and rural housing areas were found: the chi-square statistic is 19.541, p<0.005, and the result is significant at p≤0.05. For the gut microbiome and related to the housing location, the chi-square statistic is 53.68. The p<0.0001.The result is significant at p<0.05, and the differences between females and males have a chi-square statistic of 48.22. The p<0.0001. The result is significant at p<0.05. Conclusions: Significant differences were revealed between the culture-dependent microbial isolates and strains across the regional study, with variation associated with the housing location factor and the infant’s sex. These findings suggest that the infant intestinal microbiota is dynamic and that its diversity is shaped by a combination of biological sex-related factors and environmental conditions linked to the place of residence.
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References
Q. Cui, X. Zhang, J. Shao, W. Ni, Y. Yang, and B. Yan, "Bioactivities of dietary polyphenols and their effects on intestinal microbiota," Mini-Reviews in Medicinal Chemistry, vol. 23, no. 3, pp. 361–377, 2023.
K. Hou, Z.-X. Wu, X.-Y. Chen, J.-Q. Wang, D. Zhang, C. Xiao, D. Zhu, J. B. Koya, L. Wei, J. Li, et al., "Microbiota in health and diseases," Signal Transduction and Targeted Therapy, vol. 7, no. 1, Art no. 135, 2022.
R. Cantón, P. D. L. Ramos, A. García-Botella, A. García-Lledó, T. Hernández-Sampelayo, J. Gómez-Pavón, J. G. Del Castillo, M. C. Martín-Delgado, F. J. M. Sánchez, M. Martínez-Sellés, et al., "Human intestinal microbiome: Role in health and disease," Revista Española de Quimioterapia, vol. 37, no. 6, pp. 438–453, 2024.
M. D. Carretta, J. Quiroga, R. López, M. A. Hidalgo, and R. A. Burgos, "Participation of short-chain fatty acids and their receptors in gut inflammation and colon cancer," Frontiers in Physiology, vol. 12, Art no. 662739, Apr. 2021.
A. B. Padilla, S. Sahai, R. R. Pizarro, J. J. J. Añonuevo, and K. Enamno, "Human microbiome and mental health," in Human Microbiome Drug Targets. Elsevier, 2025, pp. 55–68.
N. T. Mueller, M. K. Differding, H. Sun, J. Wang, S. Levy, V. Deopujari, L. J. Appel, M. J. Blaser, T. Kundu, A. A. Shah, et al., “Maternal bacterial engraftment in multiple body sites of cesarean section born neonates after vaginal seeding-a randomized controlled trial,” mBio, vol. 14, no. 3, Art no. e00491-23, 2023.
D. A. Edwar, E. N. Naji, and A. Maleki, "Biofilm and hemolysis profile index in bacteria isolated from pre-cesarean surgery and post cesarean infections," Al-Mustansiriyah Journal of Science, vol. 34, no. 4, pp. 8–18, 2023.
H. F. Hetta, N. Sirag, H. Elfadil, A. Salama, S. F. Aljadrawi, A. J. Alfaifi, A. N. Alwabisi, B. M. AbuAlhasan, L. S. Alanazi, Y. A. Aljohani, et al., "Artificial sweeteners: A double-edged sword for gut microbiome," Diseases, vol. 13, no. 4, Art no. 115, 2025.
Y. N. Ramadan, S. F. Alqifari, K. Alshehri, A. Alhowiti, H. Mirghani, T. Alrasheed, F. Aljohani, A. Alghamdi, and H. F. Hetta, "Microbiome gut-brain-axis: Impact on brain development and mental health," Molecular Neurobiology, vol. 62, no. 8, pp. 10813–10833, 2025.
K. Berding, K. Vlckova, W. Marx, H. Schellekens, C. Stanton, G. Clarke, F. Jacka, T. G. Dinan, and J. F. Cryan, "Diet and the microbiota–gut–brain axis: Sowing the seeds of good mental health," Advances in Nutrition, vol. 12, no. 4, pp. 1239–1285, 2021.
W. Nori, N. N. Akram, M. M. Al-kaabi, R. M. Al-Ani, and A. C. Pantazi, "Innovative gut microbiome-targeted strategies: A promising frontier for pediatric inflammatory bowel disease," New Emirates Medical Journal, vol. 5, Art no. e02506882356553, Mar. 2025.
P. T. Santana, S. L. B. Rosas, B. E. Ribeiro, Y. Marinho, and H. S. P. de Souza, "Dysbiosis in inflammatory bowel disease: Pathogenic role and potential therapeutic targets," International Journal of Molecular Sciences, vol. 23, no. 7, Art no. 3464, 2022.
R. Jokela, K. M. Pärnänen, A. J. Ponsero, L. Lahti, K.-L. Kolho, W. M. de Vos, and A. Salonen, "A cohort study in family triads: Impact of gut microbiota composition and early life exposures on intestinal resistome during the first two years of life," Gut Microbes, vol. 16, no. 1, Art no. 2383746, 2024.
I. Martínez, J. C. Stegen, M. X. Maldonado-Gómez, A. M. Eren, P. M. Siba, A. R. Greenhill, and J. Walter, "The gut microbiota of rural Papua New Guineans: Composition, diversity patterns, and ecological processes," Cell Reports, vol. 11, no. 4, pp. 527–538, 2015.
E. Vinogradova, N. Mukhanbetzhanov, M. Nurgaziyev, Z. Jarmukhanov, R. Aipova, A. Sailybayeva, M. Bekbossynova, S. Kozhakhmetov, and A. Kushugulova, "Impact of urbanization on gut microbiome mosaics across geographic and dietary contexts," mSystems, vol. 9, no. 10, Art no. e00585-24, 2024.
N. M. Elsherbiny, M. Ramadan, N. H. Abu Faddan, E. A. Hassan, M. E. Ali, A. S. E.-D. Abd El-Rehim, W. A. Abbas, M. A. Abozaid, E. Hassanin, G. A. Mohamed, et al., "Impact of geographical location on the gut microbiota profile in Egyptian children with type 1 diabetes mellitus: A pilot study," International Journal of General Medicine, vol. 15, pp. 6173–6187, Jul. 2022.
Y. N. Ramadan, S. Q. Bukhari, Z. Alatawi, G. Oriquat, N. H. A. Ellah, E. H. A. Mohamedosman, R. Ahmed, and H. F. Hetta, "Evolutionary genomics of human gut bacteria: Ecological plasticity across the mutualism-pathogenicity spectrum," International Journal of Molecular Sciences, vol. 27, no. 11, Art no. 5009, 2026.
S. Delgado, A. Suárez, and B. Mayo, "Identification of dominant bacteria in feces and colonic mucosa from healthy Spanish adults by culturing and by 16S rDNA sequence analysis," Digestive Diseases and Sciences, vol. 51, no. 4, pp. 744–751, 2006.
M. Radwan Ali and A. Mohammed Khudhair, "Detection of colony adhesion factors and genetic background of adhesion genes among multidrug-resistant uropathogenic Escherichia coli isolated in Iraq," Journal of Pure and Applied Microbiology, vol. 12, no. 4, pp. 2017–2025, 2018.
R. S. Ibraheem, E. N. Naji, and I. N. Khelkal, "Genetic diversity of gut microbiota in a sample of Iraqi healthy infants," Gene Reports, vol. 44, Art no. 102577, Sep. 2026.
F. Hamed and J. K. Isa, "Isolation of Lactobacillus spp. from healthy infants' feces and study of their antibiotic susceptibility," Wasit Journal for Pure Sciences, vol. 3, no. 3, pp. 208–215, 2024.
R. Albesharat, M. A. Ehrmann, M. Korakli, S. Yazaji, and R. F. Vogel, "Phenotypic and genotypic analyses of lactic acid bacteria in local fermented food, breast milk and faeces of mothers and their babies," Systematic and Applied Microbiology, vol. 34, no. 2, pp. 148–155, 2011.
K. A. Rodriguez, M. Gurung, R. Talatala, J. R. Rearick, M. L. Ruebel, K. E. Stephens, and L. Yeruva, "The role of early life gut mycobiome on child health," Advances in Nutrition, vol. 15, no. 3, Art no. 100185, 2024.
J. Ding, X. Ma, H. Ren, C. Zhi, Q. Xin, Z. Li, L. Han, X. Zhao, Z. Zhuo, A. Li, et al., Delivery, feeding and gender differently contribute to infant gut microbiota development, Aug. 2021.
G. Ma, Y. Shi, L. Meng, H. Fan, X. Tang, H. Luo, D. Wang, J. Zhou, and X. Xiao, "Factors affecting the early establishment of neonatal intestinal flora and its intervention measures," Frontiers in Cellular and Infection Microbiology, vol. 13, Art no. 1295111, Dec. 2023.
J. Chen, H. Li, S. M. Hird, M.-H. Chen, W. Xu, K. Maas, and X. Cong, "Sex differences in gut microbial development of preterm infant twins in early life: A longitudinal analysis," Frontiers in Cellular and Infection Microbiology, vol. 11, Art no. 671074, Aug. 2021.
X. Cong, W. Xu, S. Janton, W. A. Henderson, A. Matson, J. M. McGrath, K. Maas, and J. Graf, "Gut microbiome developmental patterns in early life of preterm infants: Impacts of feeding and gender," PLOS ONE, vol. 11, no. 4, Art no. e0152751, 2016.
G. Galazzo, N. van Best, L. Bervoets, I. O. Dapaah, P. H. Savelkoul, M. W. Hornef, E. K. Hutton, K. Morrison, A. C. Holloway, H. McDonald, et al., "Development of the microbiota and associations with birth mode, diet, and atopic disorders in a longitudinal analysis of stool samples, collected from infancy through early childhood," Gastroenterology, vol. 158, no. 6, pp. 1584–1596, 2020.
M. Li, Y. Xue, H. Lu, J. Bai, L. Cui, Y. Ning, Q. Yuan, X. Jia, and S. Wang, “Relationship between infant gastrointestinal microorganisms and maternal microbiome within 6 months of delivery,” Microbiology Spectrum, vol. 12, no. 10, Art no. e03608-23, 2024.
N. Nurminen, Y.-M. Fan, E. Kortekangas, J. Lin, L. Hallamaa, K. Maleta, K.-M. Lehto, O. H. Laitinen, A. Sinkkonen, J. Lempainen, et al., "Early-life immunological and microbial differences between East African and North European children," Communications Medicine, vol. 6, no. 1, Art no. 216, 2026.
B. F. Ortutu, A. O. Okin, K. O. Darkwah, U. M. Onuorah, A. Y. Maigoro, and G. O. Iheme, "Gut microbiota and brain aging: A comparative review of African and Western populations," Frontiers in Aging Neuroscience, vol. 18, Art no. 1740408, Feb. 2026.
G. Zhao, L. Xie, Y. Wu, B. Wang, W. Teng, Z. Sun, Q. Kao, W. Liu, X. Pi, and H. Ma, "Effects of urbanization and lifestyle habits on the intestinal microbiota of adolescents in eastern China," Frontiers in Microbiology, vol. 14, Art no. 989303, Jun. 2023.
X. Li and Z. Lu, "Quantitative measurement on urbanization development level in urban agglomerations: A case of JJJ urban agglomeration," Ecological Indicators, vol. 133, Art no. 108375, Dec. 2021.
M. A. Rubel, A. Abbas, L. J. Taylor, A. Connell, C. Tanes, K. Bittinger, V. N. Ndze, J. Y. Fonsah, E. Ngwang, A. Essiane, et al., "Lifestyle and the presence of helminths is associated with gut microbiome composition in Cameroonians," Genome Biology, vol. 21, no. 1, Art no. 122, 2020.
A. Severino, E. Tohumcu, L. Tamai, P. Dargenio, S. Porcari, D. Rondinella, I. Venturini, M. Maida, A. Gasbarrini, G. Cammarota, et al., "The microbiome-driven impact of Western diet in the development of noncommunicable chronic disorders," Best Practice & Research Clinical Gastroenterology, vol. 72, Art no. 101923, Sep. 2024.
Z. Huang, Y. Li, H. Park, M. Ho, K. Bhardwaj, N. Sugimura, H. W. Lee, H. Meng, M. P. Ebert, K. Chao, et al., "Unveiling and harnessing the human gut microbiome in the rising burden of non-communicable diseases during urbanization," Gut Microbes, vol. 15, no. 1, Art no. 2237645, 2023.
L. D. D'Aloisio, M. Ballal, S. Ghosh, N. Haskey, N. Abulizi, R. Karimianghadim, C. Liu, P. Sruthi, L. Nagarajan, S. Vasudevan, et al., "The adoption of a westernized gut microbiome in Indian immigrants and Indo-Canadians is associated with dietary acculturation," npj Biofilms and Microbiomes, vol. 11, no. 1, Art no. 151, 2025.
A. Asenova, H. Hristova, T. Nedeva, P. Hristova, I. Zhivkova, and I. Rasheva, "The role of lactic acid bacteria microbiota in determining the pH value of newborn faeces," IOP Conference Series: Earth and Environmental Science, vol. 1305, no. 1, Art no. 012015, 2024.
F. Bäckhed, J. Roswall, Y. Peng, Q. Feng, H. Jia, P. Kovatcheva-Datchary, Y. Li, Y. Xia, H. Xie, H. Zhong, et al., "Dynamics and stabilization of the human gut microbiome during the first year of life," Cell Host & Microbe, vol. 17, no. 5, pp. 690–703, 2015.
T. Bankole and Y. Li, "The early-life gut microbiome in common pediatric diseases: Roles and therapeutic implications," Frontiers in Nutrition, vol. 12, Art no. 1597206, May 2025.
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