Biofilm and Hemolysis Profile Index in Bacteria Isolated from Pre-Cesarean Surgery and Post Cesarean Infections

Authors

  • Dalia Awni Edwar Department of Biology, College of Science, Mustansiriyah University, 10052 Baghdad, IRAQ.
  • Eman N. Naji Department of Biology, College of Science, Mustansiriyah University, 10052 Baghdad, IRAQ.
  • Abbas Maleki Clinical Microbiology Research Center, Ilam University of Medical Sciences, Iran.

DOI:

https://doi.org/10.23851/mjs.v34i4.1341

Keywords:

postoperative infection, bacterial hemolysis, biofilm formation, skin microbiota

Abstract

Swabs were taken from 340 different sites of skin divided into four groups including; group 1/160 swabs, group 2/50 swabs, group 3/100 swabs, group 4/30 swabs, taken from 100 female patients’ skin before surgery at the site of cesarean incision from several positions before and after sterilization with 10% povidone-iodine and with 10% povidone-iodine mixed with 70% ethanol, and from infected surgical sites. The bacterial isolates were identified by phenotypic examination, biochemical tests, as well as VITEK-2 assay. Staphylococcus epidermidis was the prevalent bacteria isolated from skin sample sources in group 1,2, and 3 with a total rate 81% followed by Staphylococcus aureus which was dominant in group 4 that included surgical site infection swabs. In addition, other bacteria species were isolated from different skin sites such as Staphylococcus haemolyticus, Kocuria kristinae, Enterobacter cloacae, Aerococcus viridans, Pantoea, and Burkholderia cepacian. Most isolates had hemolytic activity and all of them showed Beta hemolysis except Aerococcus viridans that expressed alpha hemolysis. Most isolates from numerous bacterial groups showed moderate biofilm production.

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References

R. Coico, Immunology: a short course. John Wiley & Sons, 2021.

S. Jolivet and J.-C. Lucet, "Surgical field and skin preparation," Orthopaedics & Traumatology: Surgery & Research, vol. 105, no. 1, pp. S1-S6, 2019.

CrossRef | PubMed

M. Ziaee, R. Vafaeenejad, G. Bakhtiari, I. Mostafavi, M. Gheibi, J. Fathabadi, and M. T. Ahmadi, "National Nosocomial Infection Surveillance System-based study in north eastern of Iran," Social Determinants of Health, vol. 3, no. 2, pp. 64-69, 2017.

M. Hyun, J. Y. Lee, and H. A. Kim, "Differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients," Annals of Clinical Microbiology and Antimicrobials, vol. 20, pp. 1-11, 2021.

CrossRef | PubMed

D. Styers, D. J. Sheehan, P. Hogan, and D. F. Sahm, "Laboratory-based surveillance of current antimicrobial resistance patterns and trends among Staphylococcus aureus: 2005 status in the United States," Annals of clinical microbiology and antimicrobials, vol. 5, no. 1, pp. 1-9, 2006.

CrossRef | PubMed

H.-C. Flemming and J. Wingender, "The biofilm matrix," Nature reviews microbiology, vol. 8, no. 9, pp. 623-633, 2010.

CrossRef | PubMed

K. Harika, V. P. Shenoy, N. Narasimhaswamy, and K. Chawla, "Detection of biofilm production and its impact on antibiotic resistance profile of bacterial isolates from chronic wound infections," Journal of Global Infectious Diseases, vol. 12, no. 3, p. 129, 2020.

CrossRef | PubMed

R. White and K. Cutting, "Critical colonisation of chronic wounds: microbial mechanisms," WOUNDS UK, vol. 4, no. 1, p. 70, 2008.

S. Percival, "Importance of biofilm formation in surgical infection," Journal of British Surgery, vol. 104, no. 2, pp. e85-e94, 2017.

CrossRef | PubMed

H. Zhang, Y. Zheng, H. Gao, P. Xu, M. Wang, A. Li, M. Miao, X. Xie, Y. Deng, and H. Zhou, "Identification and characterization of Staphylococcus aureus strains with an incomplete hemolytic phenotype," Frontiers in cellular and infection microbiology, vol. 6, p. 146, 2016.

CrossRef

P. R. Murray, K. S. Rosenthal, and M. A. Pfaller, Medical microbiology E-book. Elsevier Health Sciences, 2020.

G. Brooks, K. Carroll, J. Butel, S. Morse, and T. Mietzner, "Medical Microbiology. 26th edit," ed: New York: McGraw-Hill, 2013.

E. Babapour, A. Haddadi, R. Mirnejad, S.-A. Angaji, and N. Amirmozafari, "Biofilm formation in clinical isolates of nosocomial Acinetobacter baumannii and its relationship with multidrug resistance," Asian Pacific Journal of Tropical Biomedicine, vol. 6, no. 6, pp. 528-533, 2016.

CrossRef

M. S. Okee, M. L. Joloba, M. Okello, F. C. Najjuka, F. A. Katabazi, F. Bwanga, A. Nateza, and D. P. Kateeta, "Prevalence of virulence determinants in Staphylococcus epidermidis from ICU patients in Kampala, Uganda," The Journal of Infection in Developing Countries, vol. 6, no. 03, pp. 242-250, 2012.

CrossRef | PubMed

L. Pinheiro, C. Ivo Brito, A. De Oliveira, P. Yoshida Faccioli Martins, V. Cataneli Pereira, and M. d. L. Ribeiro de Souza da Cunha, "Staphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genes," Toxins, vol. 7, no. 9, pp. 3688-3699, 2015.

CrossRef | PubMed

A. A. Al-Mousawi, S. S. Al-Amara, M. N. Humoud, and S. Hattab, "Investigate the inducible clindamycin resistance in Staphylococcus haemolyticus isolated from surgical wounds infections by using D-test and molecular techniques in Al-Basrah, Iraq," Iranian Journal of War and Public Health, vol. 14, no. 3, pp. 1-7, 2022.

Y. Katayama, T. Baba, M. Sekine, M. Fukuda, and K. Hiramatsu, "Beta-hemolysin promotes skin colonization by Staphylococcus aureus," Journal of bacteriology, vol. 195, no. 6, pp. 1194-1203, 2013.

CrossRef | PubMed

Z. H. Nasser, H. H. Al-Hasnawy, and A. S. Baay, "Detection of Some Virulence Factors Genes of Staphylococcus Aureus Isolated from Patients with Ventilator-Associated Pneumonia in Al-HillaIraq," Indian Journal of Public Health, vol. 11, no. 02, p. 1987, 2020.

S. Heilbronner and T. J. Foster, "Staphylococcus lugdunensis: A skin commensal with invasive pathogenic potential," Clinical microbiology reviews, vol. 34, no. 2, pp. e00205-20, 2021.

CrossRef | PubMed

N. Giormezis, F. Kolonitsiou, A. Maki, A. Vogiatzi, M. Christofidou, E. Anastassiou, and I. Spiliopoulou, "Virulence factors among Staphylococcus lugdunensis are associated with infection sites and clonal spread," European Journal of Clinical Microbiology & Infectious Diseases, vol. 34, pp. 773-778, 2015.

CrossRef | PubMed

J. M. Al-Rifaaia, A. M. Al Haddad, and Y. Alrefaei, "TYPES OF BACTERIA FOUND IN COSMETICS USED BY FEMALE COLLEGE STUDENTS IN KUWAIT," European Journal of Biology and Medical Science Research, vol. 9, no. 4, pp. 20-34, 2021.

N. Mohamed, M. Jabber, and Z. M. Al-Jumaa, "Biotyping of Acinetobacter baumannii Iraqi Isolates," INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, vol. 3, no. 3, pp. 70-81, 2020.

N. K. Tektook, R. H. Essa, and S. S. Hussain, Investigation of Some Genes Responsible for Biofilm Formation: In Staphylococcus Epidermidis Isolated From Clinical Samples. LAP LAMBERT Academic Publishing, 2018.

M. Mateo, J. R. Maestre, L. Aguilar, M. J. Giménez, J. J. Granizo, and J. Prieto, "Strong slime production is a marker of clinical significance in Staphylococcus epidermidis isolated from intravascular catheters," European Journal of Clinical Microbiology & Infectious Diseases, vol. 27, no. 4, pp. 311-314, 2008/04/01 2008.

CrossRef | PubMed

C. E. Farran, A. Sekar, A. Balakrishnan, S. Shanmugam, P. Arumugam, and L. J. Gopalswamy, "Prevalence of biofilm-producing Staphylococcus epidermidis in the healthy skin of individuals in Tamil Nadu, India," Indian journal of medical microbiology, vol. 31, no. 1, pp. 19-23, 2013.

CrossRef | PubMed

P. Neopane, H. P. Nepal, R. Shrestha, O. Uehara, and Y. Abiko, "In vitro biofilm formation by Staphylococcus aureus isolated from wounds of hospital-admitted patients and their association with antimicrobial resistance," International journal of general medicine, pp. 25-32, 2018.

CrossRef | PubMed

E. Q. Jabur and N. Kandala, "The Production of Biofilm from Methicillin Resistant Staphylococcus Aureus Isolated from Post-Surgical Operation Inflammation," Iraqi Journal of Science, pp. 3688-3702, 2022.

CrossRef

A. R. Hiawy and J. H. Mukharmish, "MOLECULAR STUDY TO DETECT OF PREVALENCE BIOFILM GENES AND EFFECT OF PROBIOTIC ON THE STAPHYLOCOCCUS AUREUS ISOLATES IN AL-KUT CITY, IRAQ," Plant Archives, vol. 19, no. 1, pp. 1141-1148, 2019.

L. B. Shrestha, N. R. Bhattarai, and B. Khanal, "Comparative evaluation of methods for the detection of biofilm formation in coagulase-negative staphylococci and correlation with antibiogram," Infection and drug resistance, vol. 11, p. 607, 2018.

CrossRef | PubMed

S. Kamaci, Y. H. Bedeir, C. J. Utz, and A. J. Colosimo, "Staphylococcus lugdunensis septic arthritis following arthroscopic anterior cruciate ligament reconstruction," Case Reports in Orthopedics, vol. 2020, 2020.

CrossRef | PubMed

H. O. Eltwisy, H. O. Twisy, M. H. Hafez, I. M. Sayed, and M. A. El-Mokhtar, "Clinical infections, antibiotic resistance, and pathogenesis of Staphylococcus haemolyticus," Microorganisms, vol. 10, no. 6, p. 1130, 2022.

CrossRef | PubMed

V. Folliero, G. Franci, F. Dell, R. Giugliano, F. Foglia, R. Sperlongano, A. Filippis, E. Finamore, and M. Galdiero, "Evaluation of antibiotic resistance and biofilm production among clinical strain isolated from medical devices," International Journal of Microbiology, vol. 2021, 2021.

CrossRef | PubMed

B. B. Mohammed, "Identification of Bacterial Isolates from Hand Dryers of Malls Toilets in the City of Baghdad and Detection of their Virulence Factors," Annals of the Romanian Society for Cell Biology, vol. 25, no. 6, pp. 3847-3858, 2021.

H. J. Mea, P. V. C. Yong, and E. H. Wong, "An overview of Acinetobacter baumannii pathogenesis: Motility, adherence and biofilm formation," Microbiological research, vol. 247, p. 126722, 2021.

CrossRef | PubMed

A. HoÅ¡tacká, I. ÄŒižnár, and M. Å tefkoviÄová, "Temperature and pH affect the production of bacterial biofilm," Folia microbiologica, vol. 55, pp. 75-78, 2010.

CrossRef | PubMed

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Published

30-12-2023

Submission Dates

Received: 11/03/2023

Revised: 29/04/2023

Accepted: 22/05/2023

How to Cite

[1]
D. A. . Edwar, E. N. Naji, and A. . Maleki, “ Biofilm and Hemolysis Profile Index in Bacteria Isolated from Pre-Cesarean Surgery and Post Cesarean Infections”, Al-Mustansiriyah Journal of Science, vol. 34, no. 4, pp. 8–18, Dec. 2023.

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Section

Biological Science