Urine Catheter Sterilization from Adhered Biofilm Using Bimetallic Nanoparticles Synthesized Using the Plasma Reduction Method
DOI:
https://doi.org/10.23851/mjs.v36i2.1673Keywords:
Biofilm, MgO/ZnO, CuO/ZnO, Urinary catheters, NanocompositeAbstract
Background: Bacterial infections, particularly those in medical devices such as urinary catheters, are a significant clinical challenge due to structural differences between Gram-positive and Gram-negative bacteria influencing their susceptibility to antimicrobial agents. As antibiotic resistance increases, core/shell nanoparticles are being explored as potential alternatives. Objective: This study investigates the antibiofilm properties of MgO/ZnO and CuO/ZnO nanoparticles at concentrations of 10 and 40 µg/mL, respectively, against common bacterial pathogens, specifically Klebsiella pneumoniae, Escherichia coli, and Staphylococcus aureus, and their potential to sterilize urinary catheters. Methods: A 96-well microtiter plate was used to determine sublethal dosage levels (Sub-MIC) and minimum inhibitory concentrations (MIC) to evaluate antibacterial activity. Antibiofilm performance was evaluated by measuring biofilm reduction, while antiadhesion assays were conducted on urinary catheters to determine the ability of nanoparticles to prevent E. coli attachment. Results: The nanoparticles exhibited strain-dependent antibacterial effects. S. aureus and showed high sensitivity to CuO/ZnO NPs, while E. coli and K. pneumoniae were less susceptible. Notably, Gram-negative pneumococci demonstrated resistance to MgO/ZnO and CuO/ZnO nanoparticles. In terms of biofilm inhibition, MgO/ZnO NPs were slightly more effective against S. aureus and E. coli, whereas CuO/ZnO NPs showed superior activity against K. pneumoniae. Biofilm formation was reduced by up to 56.32% at sub-MIC concentrations. In catheter adhesion assays, MgO/ZnO NPs inhibited E. coli adhesion by 64.59%, compared to 60.92% for CuO/ZnO NPs. This development refers to the effective adhesion of CuO/ZnO NPs to the catheter surface. The study also presented an efficient, feasible and simple procedure of coatings of MgO/ZnO and CuO/ZnO NPs to prevent biofilm adhesion to catheter surfaces. Conclusions: MgO/ZnO and CuO/ZnO nanoparticles demonstrated potent anti-biofilm activity, inhibiting bacterial growth, biofilm formation, and catheter adhesion. However, more research is needed on cytotoxicity, biocompatibility, and long-term effects.
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P. K. Jha, C. Chawengkijwanich, C. Pokum, P. Soisan, and K. Techato, "Antibacterial activities of biosynthesized zinc oxide nanoparticles and silver‑zinc oxide nanocomposites using Camellia sinensis leaf extract," Trends in Sciences, vol. 20, no. 3, p. 5649, 2023.
CrossRef | Google Scholar
M. J. Imdad, F. Ahmed, Y. Zhao, X. Tang, and P. K. Malakar, "Targeting bacterial spores with metallic nanoparticles: A promising alternative for food safety," Current Opinion in Food Science, vol. 62, p. 101273, Apr. 2025.
CrossRef | Google Scholar
O. A. Rugaie, A. A. H. Abdellatif, M. A. El‑Mokhtar, M. A. Sabet, A. Abdelfattah, M. Alsharidah, M. Aldubaib, H. Barakat, S. M. Abudoleh, K. A. Al‑Regaiey, and H. M. Tawfeek, "Retardation of bacterial biofilm formation by coating urinary catheters with metal nanoparticle‑stabilized polymers," Microorganisms, vol. 10, no. 7, p. 1297, 2022.
CrossRef | Google Scholar | PubMed
M. Al-Qahtani, A. Safan, G. Jassim, and S. Abadla, "Efficacy of anti‑microbial catheters in preventing catheter associated urinary tract infections in hospitalized patients: A review on recent updates," Journal of Infection and Public Health, vol. 12, no. 6, pp. 760–766, 2019.
CrossRef | Google Scholar | PubMed
P. Singha, J. Locklin, and H. Handa, "A review of the recent advances in antimicrobial coatings for urinary catheters," Acta Biomaterialia, vol. 50, pp. 20–40, Mar. 2017.
CrossRef | Google Scholar | PubMed
R. Fink, H. Gilmartin, A. Richard, E. Capezuti, M. Boltz, and H. Wald, "Indwelling urinary catheter management and catheter‑associated urinary tract infection prevention practices in Nurses Improving Care for Healthsystem Elders hospitals," American Journal of Infection Control, vol. 40, no. 8, pp. 715–720, 2012.
CrossRef | Google Scholar | PubMed
J. Lo, D. Lange, and B. Chew, "Ureteral stents and foley catheters‑associated urinary tract infections: The role of coatings and materials in infection prevention," Antibiotics, vol. 3, no. 1, pp. 87–97, 2014.
CrossRef | Google Scholar | PubMed
Y. He, J. Zhao, L. Wang, C. Han, R. Yan, P. Zhu, T. Qian, S. Yu, X. Zhu, and W. He, "Epidemiological trends and predictions of urinary tract infections in the global burden of disease study 2021," Scientific Reports, vol. 15, no. 1, p. 4702, 2025.
CrossRef | Google Scholar | PubMed
D. Banaszek, T. Inglis, L. Ritchie, L. Belanger, T. Ailon, R. Charest‑Morin, N. Dea, B. K. Kwon, S. Paquette, C. G. Fisher, M. F. Dvorak, and J. T. Street, "Effectiveness of silver alloy‑coated silicone urinary catheters in patients with acute traumatic cervical spinal cord injury: Results of a quality improvement initiative," Journal of Clinical Neuroscience, vol. 78, pp. 135–138, Aug. 2020.
CrossRef | Google Scholar | PubMed
A. Mishra, A. Aggarwal, and F. Khan, "Medical device‑associated infections caused by biofilm‑forming microbial pathogens and controlling strategies," Antibiotics, vol. 13, no. 7, p. 623, 2024.
CrossRef | Google Scholar | PubMed
S. Fulaz, H. Devlin, S. Vitale, L. Quinn, J. P. O'Gara, and E. Casey, "Tailoring nanoparticle‑biofilm interactions to increase the efficacy of antimicrobial agents against Staphylococcus aureus," International Journal of Nanomedicine, vol. 15, pp. 4779–4791, Jul. 2020.
CrossRef | Google Scholar | PubMed
A. N. Olaimat, A. M. Ababneh, M. Al‑Holy, A. Al‑Nabulsi, T. Osaili, M. Abughoush, M. Ayyash, and R. A. Holley, "A review of bacterial biofilm components and formation, detection methods, and their prevention and control on food contact surfaces," Microbiology Research, vol. 15, no. 4, pp. 1973–1992, 2024.
CrossRef | Google Scholar
B. Wang, L. Du, B. Dong, E. Kou, L. Wang, and Y. Zhu, "Current knowledge and perspectives of phage therapy for combating refractory wound infections," International Journal of Molecular Sciences, vol. 25, no. 10, p. 5465, 2024.
CrossRef | Google Scholar | PubMed
A. David, A. Tahrioui, A.-S. Tareau, A. Forge, M. Gonzalez, E. Bouffartigues, O. Lesouhaitier, and S. Chevalier, "Pseudomonas aeruginosa biofilm lifecycle: involvement of mechanical constraints and timeline of matrix production," Antibiotics, vol. 13, no. 8, p. 688, 2024.
CrossRef | Google Scholar | PubMed
F. Chutrakulwong, K. Thamaphat, S. Tantipaibulvut, and P. Limsuwan, "In situ deposition of green silver nanoparticles on urinary catheters under photo‑irradiation for antibacterial properties," Processes, vol. 8, no. 12, p. 1630, 2020.
CrossRef | Google Scholar
A. L. Flores‑Mireles, J. N. Walker, M. Caparon, and S. J. Hultgren, "Urinary tract infections: Epidemiology, mechanisms of infection and treatment options," Nature Reviews Microbiology, vol. 13, no. 5, pp. 269–284, 2015.
CrossRef | Google Scholar | PubMed
S. Albu, S. Voidazan, D. Bilca, M. Badiu, A. Truţă, M. Ciorea, A. Ichim, D. Luca, and G. Moldovan, "Bacteriuria and asymptomatic infection in chronic patients with indwelling urinary catheter: The incidence of ESBL bacteria," Medicine, vol. 97, no. 33, p. e11796, 2018.
CrossRef | Google Scholar | PubMed
M. M. Kazi, A. Harshe, H. Sale, D. Mane, M. Yande, and S. Chabukswar, "Catheter associated urinary tract infections (CAUTI) and antibiotic sensitivity pattern from confirmed cases of CAUTI in a tertiary care hospital: A prospective study," Clinical Microbiology: Open Access, vol. 4, no. 2, p. 1000193, 2015.
CrossRef | Google Scholar
L. Wang, S. Zhang, R. Keatch, G. Corner, G. Nabi, S. Murdoch, F. Davidson, and Q. Zhao, "In‑vitro antibacterial and anti‑encrustation performance of silver‑polytetrafluoroethylene nanocomposite coated urinary catheters," Journal of Hospital Infection, vol. 103, no. 1, pp. 55–63, 2019.
CrossRef | Google Scholar | PubMed
J. F. B. Rodrigues, J. A. d. Silva, R. P. Medeiros, J. V. S. d. A. Queiroz, M. R. d. O. Pinto, S. K. S. Amoah, and M. V. L. Fook, "A GC‑FID validated method for detection and quantification of ethylene oxide in urine bags," Matéria (Rio de Janeiro), vol. 28, no. 1, p. e13234, 2023.
CrossRef | Google Scholar
C. V. Gould, C. A. Umscheid, R. K. Agarwal, G. Kuntz, and D. A. Pegues, "Guideline for prevention of catheter‑associated urinary tract infections 2009," Infection Control & Hospital Epidemiology, vol. 31, no. 4, pp. 319–326, 2010.
CrossRef | Google Scholar | PubMed
R. S. Fernandez and R. D. Griffiths, "Duration of short‑term indwelling catheters—a systematic review of the evidence," Journal of Wound, Ostomy and Continence Nursing, vol. 33, no. 2, pp. 145–153, 2006.
CrossRef | Google Scholar | PubMed
C. G. Kumar and P. Sujitha, "Green synthesis of Kocuran‑functionalized silver glyconanoparticles for use as antibiofilm coatings on silicone urethral catheters," Nanotechnology, vol. 25, no. 32, p. 325101, 2014.
CrossRef | Google Scholar | PubMed
B. W. Trautner and R. O. Darouiche, "Catheter‑associated infections: Pathogenesis affects prevention," Archives of Internal Medicine, vol. 164, no. 8, p. 842, 2004.
CrossRef | Google Scholar | PubMed
K. G. Neoh, M. Li, E.-T. Kang, E. Chiong, and P. A. Tambyah, "Surface modification strategies for combating catheter‑related complications: recent advances and challenges," Journal of Materials Chemistry B, vol. 5, no. 11, pp. 2045–2067, 2017.
CrossRef | Google Scholar | PubMed
S. A. Hamza, M. F. Al‑Marjani, and R. S. Mohammed, "Effect of nonthermal plasma on DNA integrity of carbapenem‑resistant Klebsiella pneumoniae," The European Physical Journal Plus, vol. 139, no. 11, p. 1023, 2024.
CrossRef | Google Scholar
M. Charnley, M. Textor, and C. Acikgoz, "Designed polymer structures with antifouling‑antimicrobial properties," Reactive and Functional Polymers, vol. 71, no. 3, pp. 329–334, 2011.
CrossRef | Google Scholar
K. B. Martins, A. M. Ferreira, V. C. Pereira, L. Pinheiro, A. d. Oliveira, and M. d. L. R. d. S. d. Cunha, "In vitro effects of antimicrobial agents on planktonic and biofilm forms of Staphylococcus saprophyticus isolated from patients with urinary tract infections," Frontiers in Microbiology, vol. 10, p. 40, Jan. 2019.
CrossRef | Google Scholar | PubMed
N. Høiby, T. Bjarnsholt, M. Givskov, S. Molin, and O. Ciofu, "Antibiotic resistance of bacterial biofilms," International Journal of Antimicrobial Agents, vol. 35, no. 4, pp. 322–332, 2010.
CrossRef | Google Scholar | PubMed
C. R. Mendes, G. Dilarri, C. F. Forsan, V. d. M. R. Sapata, P. R. M. Lopes, P. B. de Moraes, R. N. Montagnolli, H. Ferreira, and E. D. Bidoia, "Antibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogens," Scientific Reports, vol. 12, no. 1, p. 2658, 2022.
CrossRef | Google Scholar | PubMed
R. S. Ahmed, R. S. Mohammed, A. M. Abdul Majeed, and A. Sudhakaran, "Biological activity of MgO nanoparticle synthesis by plasma‑assisted reduction method," Physica Scripta, vol. 99, no. 11, p. 115901, 2024.
CrossRef | Google Scholar
R. S. Mohammed, K. A. Aadim, and K. A. Ahmed, "Synthesis of CuO/ZnO and MgO/ZnO core/shell nanoparticles with plasma jets and study of their structural and optical properties," Karbala International Journal of Modern Science, vol. 8, no. 2, pp. 88–97, 2022.
CrossRef | Google Scholar
L. M. Mahlaule‑Glory, Z. Mbita, M. M. Mathipa, Z. N. Tetana, and N. C. Hintsho‑Mbita, "Biological therapeutics of AgO nanoparticles against pathogenic bacteria and A549 lung cancer cells," Materials Research Express, vol. 6, no. 10, p. 105402, 2019.
CrossRef | Google Scholar
L. K. Vestby, T. Grønseth, R. Simm, and L. L. Nesse, "Bacterial biofilm and its role in the pathogenesis of disease," Antibiotics, vol. 9, no. 2, p. 59, 2020.
CrossRef | Google Scholar | PubMed
W. R. Talib, A. Sudhakaran, A. Sudhakaran, and R. S. Mohammed, "Potential biological and optoelectronic applications of AgO:ZnO nanocomposite synthesized by green approach," The European Physical Journal Plus, vol. 139, no. 12, p. 1118, 2024.
CrossRef | Google Scholar
V. S. Gondil and S. Chhibber, "Exploring potential of phage therapy for tuberculosis using model organism," Biomedical and Biotechnology Research Journal (BBRJ), vol. 2, no. 1, p. 9, 2018.
CrossRef | Google Scholar
R. S. Ahmed, A. M. Dahham, N. K. Abdalameer, and R. S. Mohammed, "Optical, structural and biological properties of reduced silver oxide nanoparticles from anethum graveolens leaf extract by nonthermal plasma," Nano LIFE, vol. 15, no. 5, p. 2450025, 2024.
CrossRef | Google Scholar
R. S. Mohammed and M. F. Al -Marjani, "DBD plasma as a practical approach to sterilization of dental instruments," Physica Scripta, vol. 99, no. 4, p. 045601, 2024.
CrossRef | Google Scholar
A. Sudhakaran, A. Sudhakaran, and E. Sivasenthil, "Structure-property connections in dual-phase multifunctional barium titanate based nanocomposites: Insights from charge density distributions," Materials Chemistry and Physics, vol. 318, p. 129290, May 2024.
CrossRef | Google Scholar
D. de Lacerda Coriolano, J. B. de Souza, E. V. Bueno, S. M. d. F. R. Medeiros, I. D. L. Cavalcanti, and I. M. F. Cavalcanti, "Antibacterial and antibiofilm potential of silver nanoparticles against antibiotic‑sensitive and multidrug‑resistant Pseudomonas aeruginosa strains," Brazilian Journal of Microbiology, vol. 52, no. 1, pp. 267–278, 2020.
CrossRef | Google Scholar | PubMed
S. Gurunathan, J. W. Han, D.-N. Kwon, and J.-H. Kim, "Enhanced antibacterial and anti‑biofilm activities of silver nanoparticles against Gram‑negative and Gram‑positive bacteria," Nanoscale Research Letters, vol. 9, no. 1, p. 373, 2014.
CrossRef | Google Scholar | PubMed
P. Singh, S. Pandit, J. Garnæs, S. Tunjic, V. Mokkapati, A. Sultan, A. Thygesen, A. Mackevica, R. V. Mateiu, A. E. Daugaard, A. Baun, and I. Mijakovic, "Green synthesis of gold and silver nanoparticles from Cannabis sativa (industrial hemp) and their capacity for biofilm inhibition," International Journal of Nanomedicine, vol. 13, pp. 3571–3591, Jun. 2018.
CrossRef | Google Scholar | PubMed
A. E. Nel, L. Mädler, D. Velegol, T. Xia, E. M. V. Hoek, P. Somasundaran, F. Klaessig, V. Castranova, and M. Thompson, "Understanding biophysicochemical interactions at the nano‑bio interface," Nature Materials, vol. 8, no. 7, pp. 543–557, 2009.
CrossRef | Google Scholar | PubMed
A. Kessler, J. Hedberg, E. Blomberg, and I. Odnevall, "Reactive oxygen species formed by metal and metal oxide nanoparticles in physiological media—a review of reactions of importance to nanotoxicity and proposal for categorization," Nanomaterials, vol. 12, no. 11, p. 1922, 2022.
CrossRef | Google Scholar | PubMed
J. Rajaram and Y. Kuthati, "Metal peroxide nanoparticles for modulating the tumor microenvironment: Current status and recent prospects," Cancers, vol. 16, no. 21, p. 3581, 2024.
CrossRef | Google Scholar | PubMed
M. Stuparu‑Cretu, G. Braniste, G.-A. Necula, S. Stanciu, D. Stoica, and M. Stoica, "Metal oxide nanoparticles in food packaging and their influence on human health," Foods, vol. 12, no. 9, p. 1882, 2023.
CrossRef | Google Scholar | PubMed
J. Zhang, P. Singh, Z. Cao, S. Rahimi, S. Pandit, and I. Mijakovic, "Polydopamine/graphene oxide coatings loaded with tetracycline and green Ag nanoparticles for effective prevention of biofilms," Applied Surface Science, vol. 626, p. 157221, Jul. 2023.
CrossRef | Google Scholar
Y. Y. Loo, Y. Rukayadi, M.-A.-R. Nor‑Khaizura, C. H. Kuan, B. W. Chieng, M. Nishibuchi, and S. Radu, "In vitro antimicrobial activity of green synthesized silver nanoparticles against selected gram‑negative foodborne pathogens," Frontiers in Microbiology, vol. 9, p. 1555, Jul. 2018.
CrossRef | Google Scholar | PubMed
Y. Xie, Y. He, P. L. Irwin, T. Jin, and X. Shi, "Antibacterial activity and mechanism of action of zinc oxide nanoparticles against Campylobacter jejuni," Applied and Environmental Microbiology, vol. 77, no. 7, pp. 2325–2331, 2011.
CrossRef | Google Scholar | PubMed
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