Study of Phenotypic and Genotypic Factors of Staphylococcus aureus Clinical Local Isolates


  • Ali Muhsin Ali Department of Biology, College of Science, Mustansiryah University. Baghdad, IRAQ.
  • Muhannad Moeen Abdallah Directorate General of Education Baghdad Rusafa 3, Ministry of Education. Baghdad, IRAQ.



Staphylococcus aureus, mecA, Biofilm formation, Virulence factors.


The isolates of Staphylococcus aureus were isolated from patients with various infections in hospitals, the isolates were identified and accurately diagnosed by phenotypic examination and biochemical tests, as well Vitek-2, and then genetic detection and diagnosis of many of the pathogenic factors associated with Staphylococcus aureus using conventional polymerase chain reaction (PCR) and testing for association by antibiotic resistance and production of some toxins by Staphylococcus aureus. After performing analysis of statistical, it was set up that the correlation coefficient of the PCR technique using virulence genes, sensitivity test to antibiotics and other virulence factors were significant at p < 0.05, but was insignificant with the biofilm production.


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Author Biography

Muhannad Moeen Abdallah, Directorate General of Education Baghdad Rusafa 3, Ministry of Education. Baghdad, IRAQ.




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How to Cite

A. M. Ali and M. M. Abdallah, “Study of Phenotypic and Genotypic Factors of Staphylococcus aureus Clinical Local Isolates”, Al-Mustansiriyah Journal of Science, vol. 33, no. 4, pp. 49–56, Dec. 2022.



Biological Science