A Study on Actinorhodin-like Substance Production by Streptomyces IQ45

Authors

DOI:

https://doi.org/10.23851/mjs.v31i3.93

Keywords:

Streptomyces, Actinorhodin, extraction

Abstract

Production of pH-pigment (actinorhodin – like substance) was ascertained from ten Streptomyses isolates. Streptomyses IQ45 isolate was only isolated which produced pH- sensitive pigment. The production of pH-sensitive pigment was detected by fuming over ammonia. After extraction of this antibiotic, a number of physiocochemical characterizations were carried out which involved (IR, UV, MP, CHN-analysis, and solubility test). Indicated that this antibiotic is an actinorhodin-like substance. TLC of the extracted substance showed a single spot with Rf value equivalent to (0.26) which was close to that of actinorhodin.

These antibiotics showed inhibitory activity against Staphylococcus aureus similar to that of actinorhodin produced by Streptomyces coelicolor A3 (2). The productivity of this antibiotics was (45 mg/L) at pH 8.5 and (40 mg/L) at pH 7 from the mycelial mat and (10 mg/L) when extracted from the liquid medium at pH7.

References

V. Betina, The chemistry and biology of antibioties. In pharmachemistry library Elserier Scientific publishing co. vol.5 (1983).

T. Eriko, R. Chakraburtty and M, J. Bibb, Antibiotic production in Streptomyces coelicolor A3(2). Mol. Microbial. 41, 1015-1028 (2001).

A. SAbbas and C. Edwards, Effect of Metals on Strepomyces coelicolor growth and Actionrhodin production. Apple. Environ ment. Microbiol .56(4), 675-680 (1990). DOI: https://doi.org/10.1128/aem.56.3.675-680.1990

CrossRef | PubMed

S. A. Waksman, The Actinomycetes, summery of current knowledge. The roiald press.co. New York (1967).

J. J. Berd, A. Aszalos, M. Bastain, and MC. K. L. Nitt, CRC Handbook of antibiotic compounds. 4CRC press, Inc. Boca Raton, USA ( 1980).

H, Hoekesma, and kreueger, W. C. kalafungin. ii chemical transformations and the absolute configuration. J. Antibiot. 29, 704-709. (1976) DOI: https://doi.org/10.7164/antibiotics.29.704

CrossRef | PubMed

M. Bibb, The regulation of antibiotic production in Streptomyces coelicolor A3(2). SGM Special lecture of microbiology in Dep of genetics ,John Innes centre, Norwich Research Part, Colney, Norwich. NR47UH, UK (1996) DOI: https://doi.org/10.1099/13500872-142-6-1335

CrossRef | PubMed

K. F. Chater, M. J. Bibb. Regulation of bacterial antibiotic production .In H. Kleinkauf and H. van Donhren (ed) products of Seccondary metabolism. Biotechnology vol. 7VCH Weinheim Germany (1997).

N. S. Egorov. Antibiotics a scientific approach. Mir Publishers. Moscow, (1985).

S. Cole, B. A. Rudd, D. A. Hopwood; C. Change and H. Floss. Biosynthesis of antibiotic actinorhodin, analysis of bloc ked mutants of Streptomyces coelicolor. J. Antibio t .X L(3), 340-379 (1987). DOI: https://doi.org/10.7164/antibiotics.40.340

CrossRef | PubMed

L. F. Fieser and K. L. Williamson, Organic experiments,5th ed. Lexington, Massachusetts. Toronto (1983).

D. A. Hopwood. Extra chromosomally determined antibiotic production, Ann.Rev. Microbiol. 41(3) 595-635 (1978). DOI: https://doi.org/10.1128/br.41.3.595-635.1977

CrossRef | PubMed

M. A. Ibrahim, The formation of tetracycline in date medium of Streptomyces aurofaciens induced by some chemical mutagens. MSc. Thesis, college of science (SAK) Saudia Arabia Kingdome. (2008).

K. Komagata. Catalogue of strains. Japan collection of microorganisms,3rd ed. Pub japan coll of micro, Tokyo (1986).

C. P. Grost-Allman., B. A. Rudd, C. Change and H. G Floss, Biosynthesis of actionrhodin determination of the point of dimerization J. Org. Chem. 46, 455-456. (1981).

CrossRef DOI: https://doi.org/10.1021/jo00315a046

D. A. Hopwood, and C. khosla, Genes for polyketide secondary metabolic pathways in microorgansim and plants In scondary metabolites their function and evolution Ciba Found. Symp 171P, 88-112. Whily, Chicheste, U.K (1992).

CrossRef DOI: https://doi.org/10.1002/9780470514344.ch6

B. Krone, A. Zeeck and H.G. Folls. Bioynthetic relationship in the naphthocyclinone series of isochromane quinone antibotics J. Org Chem 47, 2721-2724 (1982)

CrossRef DOI: https://doi.org/10.1021/jo00145a022

H. J. Kutzner and S. A. Waksman. Streptomyces coelicolor muller and Streptomyces violaceoruber Waksman and curtis, two distinctly different organisms. J. Bacteriol.78, 528-538 (1959). DOI: https://doi.org/10.1128/jb.78.4.528-538.1959

CrossRef | PubMed

R. Kirby, L. F. Wright and D. A. Hopwood. Plasmid determined antibiotic synthesis and resistance in Streptomyces colicolor. Nature.254, 265-267 (1975). DOI: https://doi.org/10.1038/254265a0

CrossRef | PubMed

A Ogilivie and W. Kersten.Quinone antibiotics, p243-263. In F. E. Han(ed), Antibiotic. 5 springer verlag, Berlin Heidelberg, New York (1979).

I, Philips and K,Shannon .Aminogly cosides resistance J.Gen Microbiol. 40, 28-35 (1984). DOI: https://doi.org/10.1093/oxfordjournals.bmb.a071943

CrossRef | PubMed

N,Tsuji, M. Aubayash, M, Y.Teroi and K. Tori. The structure of griseuins A and B, newisochromane quinone antibiocs. Tetrahydron. 32, 2207-2210 (1967).

CrossRef DOI: https://doi.org/10.1016/0040-4020(76)85133-2

J. S. A. Pyrek, O. Chmatowicz and A. Zamojsk. Naptho-and anthraqinones of Streptomces thermoviolaceus WR-141- structures and model synthases Tetrahydron. 3, 673-680 (1977). DOI: https://doi.org/10.1002/chin.197724372

CrossRef DOI: https://doi.org/10.1016/0040-4020(77)80309-8

B.A.M Rudd and D.A Hopwood. Genetics of actinorhodin biosynthesis by Streptomyces coelicolor A3 (2). J. Gen. Microbiol. 114, 35-43 (1979). DOI: https://doi.org/10.1099/00221287-114-1-35

CrossRef | PubMed

E. B. Shirling and D. Gottlieb. Methods for characterization of Streptormyces specis. Int J. sys Bacteriol 16, 313-340 (1966).

CrossRef DOI: https://doi.org/10.1099/00207713-16-3-313

H.Tanaka , Y. Koyama, T. Nagai, H. Marumo and S. Omura. Nanomycins, new antiboitics produced by a strain of Streptomyces. II structure and biosynthesis. J. Antibiot. 28, 868-875 (1975). DOI: https://doi.org/10.7164/antibiotics.28.868

CrossRef | PubMed

L. F. Wright, and D. A. Hopwood, Actinorhodin is a chromosomally determined antibiotic in Streptomyces coelicolor A3(2) S. Gen. Microbiol. 96, 289-297 (1976). DOI: https://doi.org/10.1099/00221287-96-2-289

CrossRef | PubMed

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Published

2020-08-20

How to Cite

[1]
N. A. Taher, A. S. Husen, Z. S. Mahmood, and G. J. Shanior, “A Study on Actinorhodin-like Substance Production by Streptomyces IQ45”, MJS, vol. 31, no. 3, pp. 6–13, Aug. 2020.

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Biological Science