Micronucleus Frequency in Buccal Cells of Males Exposed to Air Pollution in Kufa City


  • Farha A. Ali Shafi Department of Biology, College of Science, Mustansiriyah University, IRAQ Correspondent Author




binucleated cells, air pollution.


The aim of the present study is to explore micronuclei recurrence, as a biomarker of genomic damage in buccal cells of individuals living in polluted areas located near to the cement factory in Kufa city. Twenty four apparently healthy males residing in Kufa are enrolled in the present study, their ages between 18-30 years, as control group 24 males with same age are enrolled liv-ing in the center of Najaf city a far from cement factory. A sample of the exposed and control groups of buccal mucosa cells was collected during Spring months in 2016. The mean count of cell with micronuclei in buccal cells of the exposed group and control were (22.33 ± 0.97; 11.583 ± 0.85) respectively, the mean count of the binucleated cells of the exposed and control groups were (7.208 ± 0.71, 10.041 ± 0.84) respectively, a significant difference was detected in the ob-served frequencies of micronuclei and binnucleated cells in the buccal mucosa cells between the exposed and control group. The results indicated that exposure to air pollutants related to cement production induce cytotoxic as well as genotoxic damage in buccal mucosa cells


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WHO Air Quality Guidelines. Global Up-date 2005. World Health Organization Of-fice for Europe, Copenhagen, Den-mark.2006.

WHO Children’s health and the environ-ment in Europe: a baseline assessment. World Health Organization Regional Of-fice for Europe, Copenhagen, Den-mark.2007.

Shafi, F. A., AL-Ansari,N., Abdul Majeed, B. Frequency of micronuclei in peripheral blood lymphocytes of healthy individuals: A study from Baghdad, Iraq. World Jour-nal of Experimental Biosciences, 4: 40-48, 2016.

Kapka L, Baumgartner A ,Ewa Siwin S ,. Knudsen L. E, Anderson D and _zyn´ska D MEnvironmental lead exposure increas-es micronuclei in children. Mutagenesis, 22 (3) 201–207, 2007.

Jara-Ettinger AC, López-Tavera JC, Zavala-Cerna MG, Torres-Bugarín O Gen-otoxic Evaluation of Mexican Welders Oc-cupationally Exposed to Welding-Fumes Using the Micronucleus Test on Exfoliated

Shafi et al. Micronucleus Frequency in Buccal Cells of Males Exposed to Air Pollution in Kufa City 2017

Oral Mucosa Cells: A Cross-Sectional, Case-Control Study. PLoS ONE; 10(8) 2015.

K. Letaj, I. Elezaj, Q. Selimi and K. Kurte-shi The Effects of EnvironmentalPollution with Heavy Metals in Frequency of Micro-nuclei in Epithelial Buccal Cells of Human Population in Mitrovica. Journal of Chem-ical Health Risks; 2(3) 1- 4.2012.

Holland N, bolognesi C, Volders MK, Bonassi S, Zeiger E, Knasmueller S. The micronucleus assay in human buccal cells as a tool for Biomonitoring DNA damage: The HUMN project perspective on current status and knowledge gaps. Mutation Re-search.659,93-08.2008.

Thomas P, Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E. Buccal micronucleus cytome assay. Nat. Protoc. ; 4(6): 825–837.2009.

Bonassi,S, Hagmar, L. and Stromberg, U Chromosomal aberrations in lymphocytes predict human cancer independently of ex-posure to carcinogens. Cancer Research; 60, 1619–1625.2000.

Tolbert P E, Shy CM, Allen JW. Micronu-clei and other nuclear anomalies in buccal smears: methods development. Mutation Research.271, 69–77, 1992.

Tolbert PE, Shy CM, Allen JW. Micronu-clei and other nuclear anomalies in buccal smears: a field test in snuff users. Am. J. Epidemiol.134, 840–850,1991.

SAS. Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N. C. USA.2012.

Chen C, Arjomandi M, Qin H, Balmes J, Tager I, et al. Cytogenetic

damage in buc-cal epithelia and peripheral lymphocytes of young healthy individuals exposed to ozone. Mutagenesis.21, 131–137.2006.

Al-Hayali M. K. and AL-Harbawi S. Y. A. Effect of Cement Pollution on Creatinine and Blood Urea in Hamam Al-Alil Factory Workers Haitham L. College of Basic Ed-ucation Researchers Journal,11, 787- 797.2007.

Hediat M. H. Salama, M. M. Al-Rumaih, and M. A. Al-Dosary Effects of Riyadh cement industry pollutions on some physi-ological and morphological factors of Datura innoxia Mill. plantSaudi Journal Biology sciences; 18, 227–237,2011.

Al-Khashman,O,A, Shawabkeh,R. A, Met-als distribution in soils around the cement factory in southern Jordan. Environmental Pollution,140, 387-394, 2006.

Ceretti E, Feretti D, Viola GCV, Zerbini I, Limina RM, et al. DNA Damage in Buccal Mucosa Cells of Pre-School Children Ex-posed to High Levels of Urban Air Pollu-tants. PLoS ONE 9, 387–394,2014.

A. Nersesyan, Michael, K., Waldherra ,M. Setayesha ,T. Mišíka ,M. Wultscha ,G.. Metka F, Gustavo R. Mazzaron B., Sieg-fried K. Results of micronucleus assays with individuals who are occupationally and environmentally exposed to mercury, lead and cadmium, Mutation Re-search.770,119-139,2016.

Beckman, L., Nordenson, I., Interaction between some common genotoxic agents. Human Heredity.36, 397-401.1986.

Pool, B. L., Janowsky, I., Klein, P., Klein, R. G., Schmezer, P., Vogt-Leucht, G. Zeller, W. J., Effects of SO2 or NO(x) on toxic genotoxic properties of chemical car-cinogens. I. In vitro studies. Carcinogene-sis 9, 1237-1245.1988.

Meng, Z., Qin, G., Zhang, B., Bai, J. DNA damaging effects of sulfur dioxide deriva-tives in cells from various organs of mice. Mutagenesis, 19, 465-468.2004.

Lee, W. J., Teschke, K., Kauppinen, T., Andersen, A., Ja¨ppinen, P., Szadkowska Stanczyk, I., Pearce, N., Persson, B., Ber-geret, A., Facchini, L. Kishi, R., Kielkow-ski, D., Rix, B. A., Henneberger, P., Sunyer, Colin, D., Kogevinas, M., Boffet-ta, P., Mortality from lung cancerin work-ers exposed to sulfur dioxide in the pulp and paper industry. Enviroin Health Per-spect.110, 991-995.2002.

Miroslav Misık a, Karol Micieta a, Martina Solenska´ a, Katarı´na Misıkova a Helena Pisarcıkova a, Siegfried Knasmuller b. In situ biomonitoring of the genotoxic effects of mixed industrial emissions using the Tradescantia micronucleus and pollen abortion tests with wild life

Abdulrasoul M. Al-Omran, Salem E. El-Maghraby, Mahmoud E. A. Nadeem, Ali M. El-Eter and Salem M. I. Al-Qahtani. Impact of Cement Dust on Some Soil Properties Around the Cement Factory in Al-Hasa Oasis, Saudi Arabia. American-Eurasian Journal of Agric. and Environ-mental sciences, 11, 840-846, 2011.


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Original Article

How to Cite

F. A. Ali Shafi, “Micronucleus Frequency in Buccal Cells of Males Exposed to Air Pollution in Kufa City”, Al-Mustansiriyah Journal of Science, vol. 28, no. 2, pp. 43–47, Apr. 2018, doi: 10.23851/mjs.v28i2.498.

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