Insinuation Salmonella Typhi for 23Na and 60Co Radioactive Sources

Authors

  • Hanaa Salih Sabaa Department of Physics, College of Science, Mustansiriyah University, 10052 Baghdad, IRAQ.
  • Nebras Rada Mohammed Density Department, Ibn Sina University of Medical and Pharmaceutical Sciences, Baghdad, Iraq.
  • Hayim Chasib Magid Al-Turath University college, 10013 Baghdad, Iraq
  • Ali Abdulwahab Ridha University of Technology and Applied Science, Rustaq College of Education, Science Department, Physics Unit, Sultanate of Oman. https://orcid.org/0000-0002-7502-8687
  • Waleed Jabar Mhana Department of Physics, College of Science, Mustansiriyah University, 10052 Baghdad, IRAQ.
  • Saad N. Ibrahim Department of Physics, College of Science, Mustansiriyah University, 10052 Baghdad, IRAQ.

DOI:

https://doi.org/10.23851/mjs.v34i4.1385

Keywords:

Radioactive sources, Gamma rays, Beta rays and Inhibition

Abstract

The study investigates the effect of radioactive sources on Salmonella typhi, a gram-negative, rod-shaped bacterium that infects various cell types. Salmonella primarily spreads through contaminated food, commonly tainted by human or animal waste. In this descriptive research, 50 S. typhi samples from diverse patients at Baghdad Hospital were utilized. The method involved identifying the bacteria using Viteck2, culturing them on Nutrient broth and Nutrient agar, and exposing them to various radioactive sources: Na23 and CO60 (1 µCi and CO60 10 µCi). Petri dishes containing Nutrient agar were used for sample plating, followed by incubation for 24 hours at 37°C. The results showed that exposure to beta and gamma rays emitted by Na23 at an activity level of 10 µCi resulted in increased radiation levels with time. Without Almmonium, the radiation doses for 1, 2, and 3 hours were 9.64 mSv, 19.29 mSv, and 28.93 mSv, respectively, leading to varying viable cell counts. With Almmonium, the doses for the same periods were significantly lower, with fewer viable cells. Similarly, exposure to CO60 (1 µCi) resulted in varied radiation levels and viable cell counts, indicating a similar trend with and without Almmonium. CO60 (10 µCi) also demonstrated an increase in radiation doses over time, displaying an impact on viable cell counts. The study concludes that radiation emitted from Sodium and Cobalt radioactive sources exhibited effectiveness in eliminating S. typhi in direct proportion to increased exposure time and dose. This research highlights the potential for radioactive sources to impact the viability of Salmonella typhi cells.

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References

P. M., How to write Results, NY: Wiesly company, p. 55, 2003.

G. Eason, B. Noble, and I. N. Sneddon, on certain integrals of Lipschitz-Hankel type involving products of Bessel functions, vol. A247, London: Phil. Trans. Roy. Soc, pp. 529-551, 1955.

CrossRef

J. C. Maxwell, A Treatise on Electricity and Magnetism, 3rd ed. ed., vol. 2, Oxford: Clarendon, pp. 68-73, 1892.

S. Jacobs and C. P. Bean, "Fine particles, thin films and exchange anisotropy, in Magnetism, vol. III, New York: Academic: G. T. Rado and H. Suhl, Eds, p. 271-350, 1963.

CrossRef

Fàbrega A, Vila J, "Salmonella enterica serovar Typhimurium skills to succeed in the host: virulence and regulation". Clinical Microbiology Reviews. 26 (2): 308-41. (2013).

CrossRef | PubMed

R. Nicole, "Title of paper with only first word capitalized," J. Name Stand. Abbrev.

LaRock, Doris L.; Chaudhary, Anu; Miller, Samuel I., "Salmonellae interactions with host processes". Nature Reviews. Microbiology. 13 (4): 191-205. (April 2015).

CrossRef | PubMed

Garai, Preeti; Gnanadhas, Divya Prakash; Chakravortty, Dipshikha "Salmonella enterica serovars Typhimurium and Typhi as model organisms". Virulence. 3 (4): 377-388. (2012).

CrossRef | PubMed

Ohad Gal-Mor, Erin C. Boyle, and Guntram A., Same species, different diseases: how and why typhoidal and non-typhoidal Salmonella enterica serovars differ, Frontier in Microbiology, (2014)

CrossRef | PubMed

Gnanendra, Shanmugam; Anusuya, Shanmugam; Natarajan, Jeyakumar, "Molecular modeling and active site analysis of SdiA homolog, a putative quorum sensor for Salmonella typhimurium pathogenecity reveals specific binding patterns of AHL transcriptional regulators". Journal of Molecular Modeling. 18 (10): 4709-4719. ISSN 1610-2940. (October 2012).

CrossRef | PubMed

Li, Yuan-yuan; Wang, Ting; Gao, Song; Xu, Guang-mei; Niu, Hua; Huang, Rui; Wu, Shu-yan, "Salmonella plasmid virulence gene spvB enhances bacterial virulence by inhibiting autophagy in a zebrafish infection model". Fish & Shellfish Immunology. 49: 252-259. (February 2016).

CrossRef | PubMed

Black PH, Kunz LJ, Swartz MN. Salmonellosis-a review of some unusual aspects. N Engl J Med. 1960; 262:811, 864, 921.

CrossRef | PubMed

Chopra AK, Peterson JW, Chary P. et al. molecular characterization of an enterotoxin from Salmonella typhimurium. Microb Pathogen. 1994; 16:85.

CrossRef | PubMed

Choi YS, Cho WJ, Yun SH, Lee SY, Park SH, Park JC, Jang EH, Shin HY, "A case of back pain caused by Salmonella spondylitis -A case report-". Korean Journal of Anesthesiology. 59 Suppl: S233-7. doi:10.4097/kjae.2010.59. S. S233. (December 2010).

CrossRef | PubMed

Wahl, A., Battesti, A., & Ansaldi, M., Prophages in Salmonella enterica: a driving force in reshaping the genome and physiology of their bacterial host? Molecular microbiology, 111(2), 303-316, (2019).

CrossRef | PubMed

Ed. Mohammed, N. R. Al-Turath University college and Sabaa, H. S., "Essentials of Biology and Biophysics with Medical Applications of Radiotherapy." Mustansiriyah University. Iraq. Amazon publishes.

Forbes, B.A.; Sahm,D.F. and Weissfeld, A.S., Baily and Scott's Diagnostic Microbiology.11thedition. Mosby, Inc. Baltimore, USA. 384-398. (2007).

Ismail, M.C.H.; Waleed, S.; Jabbar, F. and Ibrahim, K., Effect of Diode Lazer (805) nm on alpha -toxin production and antibiotic sensitivity of Staphylococcus aureus. Iraqi J. Sci Vol 53. No. 2. Pp 755-759. (2012).

Majtan, T.; Frerman, F. E.; and Kraus, J. P., Effect of cobalt on Escherichia coli metabolism and metalloporphyrin formation. Biometals. 24 (2): 335-347. (2011).

CrossRef | PubMed

Zhu, J.; Sun, X; Zhang, Z.; Tang, Q; Gu, M.; Zhang, L.; Hou, M.; Sharon, A. and Yuan, H. Effect of Ionizing Radiation on the Bacterial and Fungal Endophytes of the Halophytic Plant Kalidium schrenkianum. Microorganisms. 9(5), 1050, (2021).

CrossRef | PubMed

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Published

30-12-2023

Submission Dates

Received: 29/04/2023

Revised: 13/05/2023

Accepted: 28/05/2023

 

How to Cite

[1]
H. S. . Sabaa, N. R. Mohammed, H. C. . Magid, A. A. . Ridha, W. J. . Mhana, and S. N. Ibrahim, “Insinuation Salmonella Typhi for 23Na and 60Co Radioactive Sources”, Al-Mustansiriyah Journal of Science, vol. 34, no. 4, pp. 123–128, Dec. 2023.

Issue

Section

Physical Sciences