Evaluation of Effect of β-Glucan on Cancer Cell Lines In vitro
DOI:
https://doi.org/10.23851/mjs.v35i1.1387Keywords:
β-glucan, MTT, Anticancer, AMJ13, MCF-7Abstract
β-Glucan is linear polysaccharides containing d-glucose monomers connected by β-glycosidic linkages. Their structural variances are the result of several sources. This research project was designed to assess the anticancer activity by using β-glucan. The in vitro experiment employed breast cancer cell lines from Michigan Cancer Foundation-7 (MCF-7) and Ahmed, Murtudha, Jabriyah, 2013 (AMJ13). After 24, 48, and 72 hours in micro titration plate under completely sterile condition. Different concentrations of β-glucan (31.25, 62.5, 125, 250, 500, and 1000 μg/mL) were applied to the cancer cell lines. The MTT assay was used to check whether the cells had been inhibited. Cell viability in MCF7 and AMJ13 cells was significantly reduced by β-glucan. β-Glucan showed concentration and time-dependent growth inhibitory effects. The higher concentrations of β-glucan significantly (P<0.05) decrease the growth rate of cells, indicating that the higher concentrations were more effective at inhibiting growth.
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References
J. Ferlay, M. Ervik, F. Lam, M. Colombet, L. Mery & M. Piñeros, Global Cancer Observatory: Cancer Today. Lyon: International Agency for Research on Cancer, 2022.
H. M. Al-Khuzaay, Y. H. Aljuraisy & M. M. Al-Halbosiy, The activity of aqueous extracts of leaves and roots of dandelion on cancer cell lines. Plant archive, 2019, vol. 19, no. 2, pp. 3933-3936
WHO-fact sheet. 2021.
N. Lemonnier, G. B. Zhou, B. Prasher, M. Mukerji, Z. Chen, S. K. Brahmachari, D. Noble, C. Auffray & M. Sagner, Traditional Knowledge-based Medicine: A Review of History, Principles, and Relevance in the Present Context of P4 Systems Medicine. Prog. Prev. Med. 2017, vol. 2, e0011.
A. M. Zimmermann-Klemd, J. K. Reinhardt, M. Winker & C. Gründemann, Phytotherapy in Integrative Oncology-An Update of Promising Treatment Options. Molecules, 2022, vol. 27, no. 10, pp. 3209.
E. J. Murphy, E. Rezoagli, I. Major, N. J. Rowan & J. G. Laffey, β-Glucan metabolic and immunomodulatory properties and potential for clinical application. J. Fungi, 2020, vol. 6, pp. 356.
A. Z. Al-Saffar, A. F. Al-Shanon, S. L. Al-Brazanchi, F. A. Sabry, F. Hassan & N. A. Hadi, Phytochemical Analysis, Antioxidant and Cytotoxic Potentials of Pelargonium graveolens Extract in Human Breast Adenocarcinoma (MCF-7) Cell Line. Asian Journal of Biochemistry, 2017, vol. 12, no. 1, pp.16-26.
N. Wang, H. Liu, G. Liu, M. Li, X. He, C. Yin, Q. Tu, X. Shen, W. Bai, Q. Wang, Y. Tao & H. Yin, Yeast β-D-glucan exerts antitumor activity in liver cancer through impairing autophagy and lysosomal function, promoting reactive oxygen species production and apoptosis. Redox Biol, 2020, vol. 32, pp. 101495.
SAS. 2018. Statistical Analysis System, User's Guide. Statistical. Version 9.6th ed. SAS. Inst. Inc. Cary. N.C. USA.
A. Choromanska, J. Kulbacka, J. Harasym, R. Oledzki, A. Szewczyk & J. Saczko, High- and low-Molecular Weight oat Beta-Glucan Reveals Antitumor Activity in Human Epithelial Lung Cancer. Pathol. Oncol. Res, 2018, vol. 24, pp. 583-592.
F. Peymaeei, F. Sadeghi & M. Roudbary, Candida albicans Beta-Glucan Induce Anti- Cancer Activity of Mesenchymal Stem Cells against Lung Cancer Cell Line: An In-Vitro Experimental Study. Asian Pac J Cancer Prev, 2020, vol. 21, no. 3, pp. 837-843.
C. P. Matos, Z. Adiguzel, Y. Yildizhan, B. Cevatemre, T. B. Onder, O. Cevik, P. Nunes, L. P. Ferreira, M. D. Carvalho, D. L.Campos, F. R. Pavan, J. C. Pessoa, M. H. Garcia, A. I. Tomaz, I. Correia & C. Acilan, May iron (III) complexes containing phenanthroline derivatives as ligands be prospective anticancer agents. Eur J Med Chem. 2019, vol. 176, pp. 492-512.
J. H. Hong & H. K. Jung, Antioxidant and antitumor activities of β-glucan rich. Exopolysaccharides with different molecular weight from Paenibacillus polymyxa JB115. Korean Soc Appl Biol Chem, 2014, vol. 57, pp. 105-112.
M. J. Kim, S. Y. Hong, S. K. Kim, C. H. Cheong, H. J. Park, H. K. Chun, K. H. Jang, B. D. Yoon, C. H. Kim & S. A. Kang, β-Glucan enhanced apoptosis in human colon cancer cells SNU-C4. Nutrion Res an Practice, 2009, vol. 3, pp. 180-184.
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