Studying The Expression of Human P53 Gene in Patients Carrying Breast Cancer.
Keywords:P53 gene, Gene expression, Breast cancer.
AbstractThe gene P53 can activate DNA repair proteins when DNA has sustained damage. So, it is an important factor in aging. Also, P53 gene can also be modified by mutagens (chemicals, radiation, or viruses), increasing the likelihood for uncontrolled cell division. For this importance, we collected thirty samples from Yarmouk hospital in Baghdad, Iraq. Twenty-nine from women patients carrying breast cancer and the last one was from healthy woman, and the samples that we have taken were embedded in paraffin wax. The extracted RNA from each samples was used to check the expression of a suppressor gene (P53) via Real time PCR. We designed primers of P53 gene on encoded sequence (Exon), to make sure generation a specific PCR product of P53 gene by converting mRNA to cDNA then PCR Product. The expression of each samples was fluctuated between 0.1 fold to 0.2 fold compared with the control (the healthy sample). The highest expression showed was samples 7 (0.2 fold), and the lowest expression showed was samples 27 (0.1 fold) that compared with control expression (sample 19). While the control expression was the highest (0.25 fold) among all samples. The results indicate that tumor may affects on a suppressor gene (P53) and can decrease the gene expression, and eventually can decrease the P53 suppressor protein that can be used it in the cell protection against cancer.
Surget S, Khoury MP, Bourdon JC, "Uncovering the role of p53 splice variants in human malignancy: a clinical perspective, " OncoTargets and Therapy, vol. 7, p. 57–68, 2013. PMid:24379683 PMCid:PMC3872270
A. P. Read and T. Strachan, " Human molecular genetics, " in : Cancer Genetics., vol. 2, New York, Wiley; ISBN, Chapter 18, 1999, pp. 471-33061.
Matlashewski G, Lamb P, Pim D, Peacock J, Crawford L, Benchimol S., "Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene, " EMBO J., vol. 3 (13), p. 3257–62, 1984. PMid:6396087 PMCid:PMC557846
Isobe M, Emanuel BS, Givol D, Oren M, Croce CM., "Localization of gene for human p53 tumour antigen to band 17p13, " in Nature 320 (6057, 1986, p. 84–5.
Kern SE, Kinzler KW, Bruskin A, Jarosz D, Friedman P, Prives C, Vogelstein B, "Identification of p53 as a sequence-specific DNA-binding protein". doi: 10.1126/science.2047879., " in Science 252 (5013), 1991, p. 1708–11. https://doi.org/10.1126/science.2047879
M. D. G. D....... McBride OW, "The gene for human p53 cellular tumor antigen is located on chromosome 17 short arm, " Proc. Natl. Acad. Sci. U.S.A, vol. 83 (1), p. 130–134, 1986. https://doi.org/10.1073/pnas.83.1.130 , PMid:3001719 PMCid:PMC322805
Bourdon JC, Fernandes K, Murray-Zmijewski F, Liu G, Diot A, Xirodimas DP, Saville MK, Lane DP, "p53 isoforms can regulate p53 transcriptional activity, " Genes & Development, vol. 243 (15), p. 11452–40, 2005. https://doi.org/10.1101/gad.1339905
Ziemer MA, Mason A, Carlson DM, "Cell-free translations of proline-rich protein mRNAs, " J. Biol. Chem, vol. 257 (18), p. 11176–80, 1982. PMid:7107651
Sonoyama T, Sakai A, Mita Y, Yasuda Y, Kawamoto H, Yagi T, Yoshioka M, Mimura T, Nakachi K, Ouchida M, Yamamoto K, Shimizu K., "TP53 codon 72 polymorphism is associated with pancreatic cancer risk in males, smokers and drinkers, " Mol Med Report 489, vol. 4 (3), p. 2011, 489-95.
Alawadi S, Ghabreau L, Alsaleh M, Abdulaziz Z, Rafeek M, Akil N, Alkhalaf M., "P53 gene polymorphisms and breast cancer risk in Arab women., " Med. Oncol., vol. 28 (3), p. 709–15, 2009. https://doi.org/10.1007/s12032-010-9505-4
C. X... Harms KL, "The C Terminus of p53 Family Proteins Is a Cell Fate Determinant, " Mol. Cell. Biol., vol. 25 (5), p. 2014–30, 2005. https://doi.org/10.1128/MCB.25.5.2014-2030.2005
Piskacek S, Gregor M, Nemethova M, Grabner M, Kovarik P, Piskacek M., "Nine-amino-acid transactivation domain: establishment and prediction utilities, " Genomics, vol. 89 (6), p. 756–68, 2007.
S. S. a. T. C. 3. Milena Gasco1, " The p53 pathway in breast cancer Cancer, " DOI: 10.1186/bcr426 © BioMed Central Ltd Research 200, vol. (24): 70, 2002. https://doi.org/10.1186/bcr426
Yu H, Huang YJ, Liu Z, Wang LE, Li G, Sturgis EM, Johnson DG, Wei Q, "Effects of MDM2 promoter polymorphisms and p53 codon 72 polymorphism on risk and age at onset of squamous cell carcinoma of the head and neck, " Mol. Carcinog, vol. 50 (9), p. 697–706, 2011.
Venot C, Maratrat M, Dureuil C, Conseiller E, Bracco L, Debussche L., "The requirement for the p53 proline-rich functional domain for mediation of apoptosis is correlated with specific PIG3 gene transactivation and with transcriptional repression, " EMBO J., vol. 17 (16), p. 4668–79, 1998. https://doi.org/10.1093/emboj/17.16.4668
Larsen S, Yokochi T, Isogai E, Nakamura Y, Ozaki T, Nakagawara A., ""LMO3 interacts with p53 and inhibits its transcriptional activity, " Biochem. Biophys. Res. Commun., vol. 392 (3), p. 252–7, 2010.
Lane, edited by Arnold J. Levine, David P. The p53 family :, "a subject collection from Cold Spring Harbor Perspectives in biology. Cold Spring Harbor, N.Y., " Cold Spring Harbor Laboratory Press, 2010, pp. 87969-830.
M. E. May P, "Twenty years of p53 research: structural and functional aspects of the p53 protein, " vol. doi: 10.1038/sj.onc.1203285. Oncogene 18 (53), p. 7621–36, 1999. https://doi.org/10.1038/sj.onc.1203285
Bernal JA, Luna R, Espina A, Lázaro I, Ramos-Morales F, Romero F, Arias C, Silva A, Tortolero M, Pintor-Toro JA, "Human securin interacts with p53 and modulates p53-mediated transcriptional activity and apoptosis, " Nature Genetics, vol. 32 (2), p. 306–11, 2002. https://doi.org/10.1038/ng997
Klug SJ, Ressing M, Koenig J, Abba MC, Agoras VM, Sengupta S, Settheetham-Ishida W, Shirasawa H, Snijders PJ, Stoler MH, Suárez-Rincón AE, Szarka K, Tachezy R, Ueda M, van der Zee AG, von Knebel Doeberitz M, Wu MT, Yamashita T, Zehbe I, Blettner M., "TP53 codon 72 polymorphism and cervical cancer: a pooled analysis of individual data from 49 studies, " Lancet Oncology, vol. 10 (2), p. 772–784, 2009.
H. J. D. B. J. C. N. P. P. M. L. D. F. A. Bullock AN, "Thermodynamic stability of wild-type and mutant p53 core domain, " Proc. Natl. Acad. Sci. U.S.A., vol. 94 (26), p. 14338–42, 1997. https://doi.org/10.1073/pnas.94.26.14338
How to Cite
The journal has no restrictions for the author to hold the copyrights of his articles. The journal does not allow authors to republish the same article in other journals or conferences that is published in one of its volumes.