Spectrophotometric Determination of Doxycycline Hyclate in Pure and Capsule using Diazotization Reaction
DOI:
https://doi.org/10.23851/mjs.v27i5.167Keywords:
Doxycycline hyclate, benzocaine, spectrophotometric, Diazotization.Abstract
A sensitive spectrophotometric method has been developed for the determination of Doxycycline hyclate (DCH) as pure and capsule forms. This method is based on diazotization of primary amine group of benzocaine with sodium nitrite in hydrochloric acid medium; the formed diazonium salt is then reacted with DCH in sodium hydroxide medium, to form yellow – orange Azo dye. Beer's law is obeyed in the concentration rang 16-34mg. ml-1 at 480 nm with detection limit LOD and molar absorptivity Є were found to be 0.418 mg.ml-1 and 2.214× 10+4 L.mol-1.cm-1, respectively.
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Ramesh P. J., Basavaiah K., Tharpa K.,, J. of pre – clinical and clinical research, vol. 4, no. 2, pp. 101-107, 2010,.
Baraka M. M., Elsadek. M. M., Abdelaziz L. M,, International J. of current pharmaceutical research, vol. 6, no. 3, 2014.
Patyra E., Kowalczyk E., Bullvet Int. Pulwy, vol. 56, pp. 329-333, 2012.
Gajda A., Posniak A., Zmudzki J., J. chromatogr. B, anlyt technol. Biomed life sci., vol. 928, pp. 113-120, 2013.
Sreeran V., Nagendrakumar A.V.D, Madhu M., chemical science transactions, vol. 4, no. 1, pp. 69-74, 2015.
Lofty H. M., Hegha M. A., Mohamed E. H., spectrochimica Acta part A: molecular and bio molecular spectroscopy, vol. 153, pp. 321-332, 2016.
Li S., Li J., Lin Q., Wei X.,, Analyst J, vol. 140, pp. 4702-4707, 2015.
Gȕrler B., Özkorucklu S., Kir E., J. of pharmaceutical and biomedical analysis, vol. 84, pp. 263-268, 2013.
https://doi.org/10.1016/j.jpba.2013.06.009
PMid:23856456
Injac R., Kac J., Strukyelj B., Analytical and Bio analytical chemistry, vol. 387, no. 2, pp. 695-701, 2007.
https://doi.org/10.1007/s00216-006-0900-9
PMid:17102968
Pavagada J., Ramesh K., Divya N., Association of chemical engineers AchE, vol. 16, no. 2, pp. 139-148, 2010.
Ahmed N. R., Hassan W. E, J. Raf. Sci., vol. 20, no. 3, pp. 66-73, 2009.
Al-Abachi M. Q., Al-Nedawi Z. A., J. of Al Nahrain university, vol. 18, no. 3, pp. 24-32, 2015.
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