Spectrophotometric Determination of Doxycycline Hyclate in Pure and Capsule using Diazotization Reaction

Ruba Fahmi Abbas; Ali Amer Wheeb; Ala'a Abdullwahid Jasim

doi:10.23851/mjs.v27i5.167

Spectrophotometric Determination of Doxycycline Hyclate in Pure and Capsule using Diazotization Reaction

Authors

Ruba Fahmi Abbas Department of Chemistry, Collage of Science, Al-Mustansiriyah University, Baghdad
Ali Amer Wheeb Department of Chemistry, Collage of Science, Al-Mustansiriyah University, Baghdad
Ala'a Abdullwahid Jasim Department of Chemistry, Collage of Science, Al-Mustansiriyah University, Baghdad

DOI:

https://doi.org/10.23851/mjs.v27i5.167

Keywords:

Doxycycline hyclate, benzocaine, spectrophotometric, Diazotization.

Abstract

A sensitive spectrophotometric method has been developed for the determination of Doxycycline hyclate (DCH) as pure and capsule forms. This method is based on diazotization of primary amine group of benzocaine with sodium nitrite in hydrochloric acid medium; the formed diazonium salt is then reacted with DCH in sodium hydroxide medium, to form yellow – orange Azo dye. Beer's law is obeyed in the concentration rang 16-34mg. ml^-1 at 480 nm with detection limit LOD and molar absorptivity Є were found to be 0.418 mg.ml^-1 and 2.214× 10⁺⁴ L.mol^-1.cm^-1, respectively.

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References

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Key Dates

Published

06-07-2017

Issue

Vol. 27 No. 5 (2016)

Section

Original Article

License

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How to Cite

[1]

R. F. Abbas, A. A. Wheeb, and A. A. Jasim, “Spectrophotometric Determination of Doxycycline Hyclate in Pure and Capsule using Diazotization Reaction”, Al-Mustansiriyah Journal of Science, vol. 27, no. 5, pp. 50–54, Jul. 2017, doi: 10.23851/mjs.v27i5.167.