Spectrophotometric Determination of Doxycycline Hyclate in Pure and Capsule using Diazotization Reaction

Authors

  • Ruba Fahmi Abbas Department of Chemistry, Collage of Science, Al-Mustansiriyah University, Baghdad
  • Ali Amer Wheeb Department of Chemistry, Collage of Science, Al-Mustansiriyah University, Baghdad
  • Ala'a Abdullwahid Jasim Department of Chemistry, Collage of Science, Al-Mustansiriyah University, Baghdad

DOI:

https://doi.org/10.23851/mjs.v27i5.167

Keywords:

Doxycycline hyclate, benzocaine, spectrophotometric, Diazotization.

Abstract

A sensitive spectrophotometric method has been developed for the determination of Doxycycline hyclate (DCH) as pure and  capsule forms. This method is based on diazotization of primary amine group of benzocaine with sodium nitrite in hydrochloric acid medium; the formed diazonium salt is then reacted with DCH in sodium hydroxide medium, to form yellow – orange Azo dye. Beer's law is obeyed in the concentration rang 16-34mg. ml-1 at 480 nm with detection limit LOD and molar absorptivity Є were found to be 0.418 mg.ml-1 and 2.214× 10+4 L.mol-1.cm-1, respectively.

References

Ramesh P. J., Basavaiah K., Tharpa K.,, J. of pre – clinical and clinical research, vol. 4, no. 2, pp. 101-107, 2010,.

Baraka M. M., Elsadek. M. M., Abdelaziz L. M,, International J. of current pharmaceutical research, vol. 6, no. 3, 2014.

Patyra E., Kowalczyk E., Bullvet Int. Pulwy, vol. 56, pp. 329-333, 2012.

Gajda A., Posniak A., Zmudzki J., J. chromatogr. B, anlyt technol. Biomed life sci., vol. 928, pp. 113-120, 2013.

Sreeran V., Nagendrakumar A.V.D, Madhu M., chemical science transactions, vol. 4, no. 1, pp. 69-74, 2015.

Lofty H. M., Hegha M. A., Mohamed E. H., spectrochimica Acta part A: molecular and bio molecular spectroscopy, vol. 153, pp. 321-332, 2016.

Li S., Li J., Lin Q., Wei X.,, Analyst J, vol. 140, pp. 4702-4707, 2015.

Gȕrler B., Özkorucklu S., Kir E., J. of pharmaceutical and biomedical analysis, vol. 84, pp. 263-268, 2013.

https://doi.org/10.1016/j.jpba.2013.06.009

PMid:23856456

Injac R., Kac J., Strukyelj B., Analytical and Bio analytical chemistry, vol. 387, no. 2, pp. 695-701, 2007.

https://doi.org/10.1007/s00216-006-0900-9

PMid:17102968

Pavagada J., Ramesh K., Divya N., Association of chemical engineers AchE, vol. 16, no. 2, pp. 139-148, 2010.

Ahmed N. R., Hassan W. E, J. Raf. Sci., vol. 20, no. 3, pp. 66-73, 2009.

Al-Abachi M. Q., Al-Nedawi Z. A., J. of Al Nahrain university, vol. 18, no. 3, pp. 24-32, 2015.

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Published

2017-07-06

How to Cite

[1]
R. F. Abbas, A. A. Wheeb, and A. A. Jasim, “Spectrophotometric Determination of Doxycycline Hyclate in Pure and Capsule using Diazotization Reaction”, MJS, vol. 27, no. 5, pp. 50–54, Jul. 2017.

Issue

Section

Chemical Science