Spectrophotometric Determination of Doxycycline Hyclate in Pure and Capsule using Diazotization Reaction

Ruba Fahmi Abbas; Ali Amer Wheeb; Ala'a Abdullwahid Jasim

doi:10.23851/mjs.v27i5.167

Spectrophotometric Determination of Doxycycline Hyclate in Pure and Capsule using Diazotization Reaction

Authors

Ruba Fahmi Abbas Department of Chemistry, Collage of Science, Al-Mustansiriyah University, Baghdad
Ali Amer Wheeb Department of Chemistry, Collage of Science, Al-Mustansiriyah University, Baghdad
Ala'a Abdullwahid Jasim Department of Chemistry, Collage of Science, Al-Mustansiriyah University, Baghdad

DOI:

https://doi.org/10.23851/mjs.v27i5.167

Keywords:

Doxycycline hyclate, benzocaine, spectrophotometric, Diazotization.

Abstract

A sensitive spectrophotometric method has been developed for the determination of Doxycycline hyclate (DCH) as pure and capsule forms. This method is based on diazotization of primary amine group of benzocaine with sodium nitrite in hydrochloric acid medium; the formed diazonium salt is then reacted with DCH in sodium hydroxide medium, to form yellow – orange Azo dye. Beer's law is obeyed in the concentration rang 16-34mg. ml^-1 at 480 nm with detection limit LOD and molar absorptivity Є were found to be 0.418 mg.ml^-1 and 2.214× 10⁺⁴ L.mol^-1.cm^-1, respectively.

References

Ramesh P. J., Basavaiah K., Tharpa K.,, J. of pre – clinical and clinical research, vol. 4, no. 2, pp. 101-107, 2010,.

Baraka M. M., Elsadek. M. M., Abdelaziz L. M,, International J. of current pharmaceutical research, vol. 6, no. 3, 2014.

Patyra E., Kowalczyk E., Bullvet Int. Pulwy, vol. 56, pp. 329-333, 2012.

Gajda A., Posniak A., Zmudzki J., J. chromatogr. B, anlyt technol. Biomed life sci., vol. 928, pp. 113-120, 2013.

Sreeran V., Nagendrakumar A.V.D, Madhu M., chemical science transactions, vol. 4, no. 1, pp. 69-74, 2015.

Lofty H. M., Hegha M. A., Mohamed E. H., spectrochimica Acta part A: molecular and bio molecular spectroscopy, vol. 153, pp. 321-332, 2016.

Li S., Li J., Lin Q., Wei X.,, Analyst J, vol. 140, pp. 4702-4707, 2015.

Gȕrler B., Özkorucklu S., Kir E., J. of pharmaceutical and biomedical analysis, vol. 84, pp. 263-268, 2013.

https://doi.org/10.1016/j.jpba.2013.06.009

PMid:23856456

Injac R., Kac J., Strukyelj B., Analytical and Bio analytical chemistry, vol. 387, no. 2, pp. 695-701, 2007.

https://doi.org/10.1007/s00216-006-0900-9

PMid:17102968

Pavagada J., Ramesh K., Divya N., Association of chemical engineers AchE, vol. 16, no. 2, pp. 139-148, 2010.

Ahmed N. R., Hassan W. E, J. Raf. Sci., vol. 20, no. 3, pp. 66-73, 2009.

Al-Abachi M. Q., Al-Nedawi Z. A., J. of Al Nahrain university, vol. 18, no. 3, pp. 24-32, 2015.

Downloads

Published

2017-07-06

How to Cite

[1]

R. F. Abbas, A. A. Wheeb, and A. A. Jasim, “Spectrophotometric Determination of Doxycycline Hyclate in Pure and Capsule using Diazotization Reaction”, Al-Mustansiriyah Journal of Science, vol. 27, no. 5, pp. 50–54, Jul. 2017.

Issue

Vol. 27 No. 5 (2016)

Section

Chemical Science

License

Articles accepted for publication in Al-Mustansiriyah Journal of Science (MJS) are protected under the Creative Commons Attribution 4.0 International License (CC-BY-NC). Authors of accepted articles are requested to sign a copyright release form prior to their article being published. All authors must agree to the submission, sign copyright release forms, and agree to be included in any correspondence between MJS and the authors before submitting a work to MJS. For personal or educational use, permission is given without charge to print or create digital copies of all or portions of a MJS article. However, copies must not be produced or distributed for monetary gain. It is necessary to respect the copyright of any parts of this work that are not owned by MJS.