Broadband Cavity Enhanced Absorption Spectroscopy of thin films of haemoglobin

Authors

  • Noora Sh. Oraha Qas Nouna Mustansiriyah University, collage of Science, Department of Physics http://orcid.org/0000-0002-0260-4321
  • Asmaa S. Al-Ragehey Mustansiriyah University, collage of Science, Department of Physics
  • Saad N. Ibrahim Computer Science Department, College of Science, Mustansiriyah University,

DOI:

https://doi.org/10.23851/mjs.v30i2.592

Keywords:

Thin films, Drop coating method, Absorption spectroscopy.

Abstract

In this study, a sensitive technique (Broadband Cavity Enhanced Absorption Spectroscopy (BBCEAS)) is employed for measuring the absorption of thin films deposited onto a glass substrate. A thin film of the biological solution such as Lyophilied Bovine haemoglobin is deposited on glass microscope coverslips. Drop coating method was used to deposit a thin film over the microscope coverslips. The number of passes is calculated: 612 passes for the high reflectivity mirror were obtained. The best measurements are made with the mirror set of reflectivity of (R≥ 0.99) which produced an αmin value of 0.0043 cm-1 and LOD of 1.9×10-7M.

Author Biography

Noora Sh. Oraha Qas Nouna, Mustansiriyah University, collage of Science, Department of Physics

Physics, Molecular Physics, spectroscopy, physical chemistry

References

C. S. Thom, C. F. Dickson, D. A. Gell, and M. J. Weiss, "Hemoglobin variants: biochemical properties and clinical correlates", Cold Spring Harbor perspectives in medicine, vol. 3, pp. 1-22, 2013. [

">Crossref]

B. L. Horecker, "The absorption spectra of hemoglobin and its derivatives in the visible and near infra-red regions", J. biol. Chem, vol. 148, pp. 173-183, 1943.

W. Zijlstra, A. Buursma, and W. Meeuwsen-Van der Roest, "Absorption spectra of human fetal and adult oxyhemoglobin, de-oxyhemoglobin, carboxyhemoglobin, and methemoglobin", Clinical chemistry, vol. 37, pp. 1633-1638, 1991.

W. G. Zijlstra and A. Buursma, "Spectrophotometry of Hemoglobin: Absorption Spectra of Bovine Oxyhemoglobin, Deoxyhemoglobin, Carboxyhemoglobin, and Methemoglobin", Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, vol. 118, pp. 743-749, 1997. [

">Crossref]

G. Berden, R. Peeters, and G. Meijer, "Cavity ring-down spectroscopy: Experimental schemes and applications", International Reviews in Physical Chemistry, vol. 19, pp. 565-607, 2000. [

">Crossref]

S. Nakamura, A. Matsugi, A. Susa, and M. Koshi, "UV-visible absorption spectra of silicon CVD intermediates", Thin Solid Films, vol. 516, pp. 517-520, 2008. [

">Crossref]

R. Peeters, G. Berden, A. Olafsson, L. J. Laarhoven, and G. Meijer, "Cavity enhanced absorption spectroscopy in the 10 μm region using a waveguide CO2 laser", Chemical physics letters, vol. 337, pp. 231-236, 2001. [

">Crossref]

S. E. Fiedler, A. Hese, and A. A. Ruth, "Incoherent broad-band cavity-enhanced absorption spectroscopy", Chemical physics letters, vol. 371, pp. 284-294, 2003. [

">Crossref]

S. M. Ball, J. M. Langridge, and R. L. Jones, "Broadband cavity enhanced absorption spectroscopy using light emitting diodes", Chemical Physics Letters, vol. 398, pp. 68-74, 2004. [

">Crossref]

M. Triki, P. Cermak, G. Méjean, and D. Romanini, "Cavity-enhanced absorption spectroscopy with a red LED source for NO x trace analysis", Applied Physics B, vol. 91, pp. 195-201, 2008. [

">Crossref]

L. Van der Sneppen, A. Wiskerke, F. Ariese, C. Gooijer, and W. Ubachs, "Cavity ring-down spectroscopy for detection in liquid chromatography: extension to tunable sources and ultraviolet wavelengths", Applied spectroscopy, vol. 60, pp. 931-935, 2006. [

">Crossref]

S. E. Fiedler, A. Hese, and A. A. Ruth, "Incoherent broad-band cavity-enhanced absorption spectroscopy of liquids", Review of Scientific Instruments, vol. 76, p. 023107, 2005. [

">Crossref]

M. Islam, L. N. Seetohul, and Z. Ali, "Liquid-phase broadband cavity-enhanced absorption spectroscopy measurements in a 2 mm cuvette", Applied spectroscopy, vol. 61, pp. 649-658, 2007. [

">Crossref]

L. Van der Sneppen, F. Ariese, C. Gooijer, and W. Ubachs, "Cavity ring-down spectroscopy for detection in liquid chromatography at UV wavelengths using standard cuvettes in a normal incidence geometry", Journal of Chromatography A, vol. 1148, pp. 184-188, 2007. [

">Crossref]

R. Engeln, G. von Helden, A. J. van Roij, and G. Meijer, "Cavity ring down spectroscopy on solid C 60", The Journal of chemical physics, vol. 110, pp. 2732-2733, 1999. [

">Crossref]

G. A. Marcus and H. A. Schwettman, "Cavity ringdown spectroscopy of thin films in the mid-infrared", Applied optics, vol. 41, pp. 5167-5171, 2002. [

">Crossref]

S. L. Logunov, "Cavity ringdown detection of losses in thin films in the telecommunication wavelength window", Applied optics, vol. 40, pp. 1570-1573, 2001. [

">Crossref]

R. N. Muir and A. J. Alexander, "Structure of monolayer dye films studied by Brewster angle cavity ringdown spectroscopy", Physical Chemistry Chemical Physics, vol. 5, pp. 1279-1283, 2003. [

">Crossref]

D. Kleine, J. Lauterbach, K. Kleinermanns, and P. Hering, "Cavity ring-down spectroscopy of molecularly thin iodine layers", Applied Physics B, vol. 72, pp. 249-252, 2001. [

">Crossref]

N. Qasnouna, "Solid-Phase BBCEAS Measurements at UV wavelength", Journal of College of Education, pp. 197-206, 2016.

Downloads

Published

2019-09-30

How to Cite

[1]
N. S. Oraha Qas Nouna, A. S. Al-Ragehey, and S. N. Ibrahim, “Broadband Cavity Enhanced Absorption Spectroscopy of thin films of haemoglobin”, MJS, vol. 30, no. 2, pp. 67–72, Sep. 2019.

Issue

Section

Physical Sciences