Broadband Cavity Enhanced Absorption Spectroscopy of thin films of haemoglobin
DOI:
https://doi.org/10.23851/mjs.v30i2.592Keywords:
Thin films, Drop coating method, Absorption spectroscopy.Abstract
In this study, a sensitive technique (Broadband Cavity Enhanced Absorption Spectroscopy (BBCEAS)) is employed for measuring the absorption of thin films deposited onto a glass substrate. A thin film of the biological solution such as Lyophilied Bovine haemoglobin is deposited on glass microscope coverslips. Drop coating method was used to deposit a thin film over the microscope coverslips. The number of passes is calculated: 612 passes for the high reflectivity mirror were obtained. The best measurements are made with the mirror set of reflectivity of (R≥ 0.99) which produced an αmin value of 0.0043 cm-1 and LOD of 1.9×10-7M.Downloads
References
C. S. Thom, C. F. Dickson, D. A. Gell, and M. J. Weiss, "Hemoglobin variants: biochemical properties and clinical correlates", Cold Spring Harbor perspectives in medicine, vol. 3, pp. 1-22, 2013. [
">Crossref]
B. L. Horecker, "The absorption spectra of hemoglobin and its derivatives in the visible and near infra-red regions", J. biol. Chem, vol. 148, pp. 173-183, 1943.
W. Zijlstra, A. Buursma, and W. Meeuwsen-Van der Roest, "Absorption spectra of human fetal and adult oxyhemoglobin, de-oxyhemoglobin, carboxyhemoglobin, and methemoglobin", Clinical chemistry, vol. 37, pp. 1633-1638, 1991.
W. G. Zijlstra and A. Buursma, "Spectrophotometry of Hemoglobin: Absorption Spectra of Bovine Oxyhemoglobin, Deoxyhemoglobin, Carboxyhemoglobin, and Methemoglobin", Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, vol. 118, pp. 743-749, 1997. [
">Crossref]
G. Berden, R. Peeters, and G. Meijer, "Cavity ring-down spectroscopy: Experimental schemes and applications", International Reviews in Physical Chemistry, vol. 19, pp. 565-607, 2000. [
">Crossref]
S. Nakamura, A. Matsugi, A. Susa, and M. Koshi, "UV-visible absorption spectra of silicon CVD intermediates", Thin Solid Films, vol. 516, pp. 517-520, 2008. [
">Crossref]
R. Peeters, G. Berden, A. Olafsson, L. J. Laarhoven, and G. Meijer, "Cavity enhanced absorption spectroscopy in the 10 μm region using a waveguide CO2 laser", Chemical physics letters, vol. 337, pp. 231-236, 2001. [
">Crossref]
S. E. Fiedler, A. Hese, and A. A. Ruth, "Incoherent broad-band cavity-enhanced absorption spectroscopy", Chemical physics letters, vol. 371, pp. 284-294, 2003. [
">Crossref]
S. M. Ball, J. M. Langridge, and R. L. Jones, "Broadband cavity enhanced absorption spectroscopy using light emitting diodes", Chemical Physics Letters, vol. 398, pp. 68-74, 2004. [
">Crossref]
M. Triki, P. Cermak, G. Méjean, and D. Romanini, "Cavity-enhanced absorption spectroscopy with a red LED source for NO x trace analysis", Applied Physics B, vol. 91, pp. 195-201, 2008. [
">Crossref]
L. Van der Sneppen, A. Wiskerke, F. Ariese, C. Gooijer, and W. Ubachs, "Cavity ring-down spectroscopy for detection in liquid chromatography: extension to tunable sources and ultraviolet wavelengths", Applied spectroscopy, vol. 60, pp. 931-935, 2006. [
">Crossref]
S. E. Fiedler, A. Hese, and A. A. Ruth, "Incoherent broad-band cavity-enhanced absorption spectroscopy of liquids", Review of Scientific Instruments, vol. 76, p. 023107, 2005. [
">Crossref]
M. Islam, L. N. Seetohul, and Z. Ali, "Liquid-phase broadband cavity-enhanced absorption spectroscopy measurements in a 2 mm cuvette", Applied spectroscopy, vol. 61, pp. 649-658, 2007. [
">Crossref]
L. Van der Sneppen, F. Ariese, C. Gooijer, and W. Ubachs, "Cavity ring-down spectroscopy for detection in liquid chromatography at UV wavelengths using standard cuvettes in a normal incidence geometry", Journal of Chromatography A, vol. 1148, pp. 184-188, 2007. [
">Crossref]
R. Engeln, G. von Helden, A. J. van Roij, and G. Meijer, "Cavity ring down spectroscopy on solid C 60", The Journal of chemical physics, vol. 110, pp. 2732-2733, 1999. [
">Crossref]
G. A. Marcus and H. A. Schwettman, "Cavity ringdown spectroscopy of thin films in the mid-infrared", Applied optics, vol. 41, pp. 5167-5171, 2002. [
">Crossref]
S. L. Logunov, "Cavity ringdown detection of losses in thin films in the telecommunication wavelength window", Applied optics, vol. 40, pp. 1570-1573, 2001. [
">Crossref]
R. N. Muir and A. J. Alexander, "Structure of monolayer dye films studied by Brewster angle cavity ringdown spectroscopy", Physical Chemistry Chemical Physics, vol. 5, pp. 1279-1283, 2003. [
">Crossref]
D. Kleine, J. Lauterbach, K. Kleinermanns, and P. Hering, "Cavity ring-down spectroscopy of molecularly thin iodine layers", Applied Physics B, vol. 72, pp. 249-252, 2001. [
">Crossref]
N. Qasnouna, "Solid-Phase BBCEAS Measurements at UV wavelength", Journal of College of Education, pp. 197-206, 2016.
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