Effect of cultivation conditions on hemolysin production from clinical isolates of Serratia marcescens
Keywords:Serratia marcescens, hemolysin, cultivation condition
Sixty-four isolates were collected from clinical sources in Baghdad and Mosul city. The identification of S. marcescens confirmed by using API 20E and VITEK-2 systems. Twelve isolates of S. marcescens produced hemolysin, which were detected by two ways, qualitative hemolytic assay and quantitative hemolytic assay. The optical density of hemolysin producing isolates at standard condition (37oC, pH=7, 24h) showed that Serratia marcescens (SmU9) isolate gave the highest absorbance of hemolysin production (0.7) but at (25°C, pH=7,24h) the absorbance of hemolysin production turn into (3.0), compared with the absorbance at 571nm for Control without hemolysin (0.060) and completely hemolysis by Triton x-100 (6.0). However, the optimum optical density of the best hemolysin producing isolate (SmU9) at the optimum cultivation conditions (25°C, pH=9, 24h, 150 rpm, 1% inoculum size in Nutrient broth) was (6.0).
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