Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant
DOI:
https://doi.org/10.23851/mjs.v31i3.670Keywords:
Polycyclic aromatic hydrocarbons PAH, Immobilized laccase, Trametes versicolor, Bioremediation, HBT, Conversation, Hyacinth plant.Abstract
Polycyclic aromatic hydrocarbons (PAHs) are recognized as a toxic, mutagenic and/or carcinogenic compounds, and their pollution of soil and aquifer is of increasing environmentally risk. Laccases (E.C. 1.10.3.2) are phenoloxidases catalyze the oxidation of PAHs in the presence of a mediator compound and hyacinth plant. In this study laccase from Trametes versicolor was immobilized into chitosan, and the potential to oxidize anthracene in the presence of 1-hydroxybenzotriazole (HBT) was examined. Results indicated that the immobilization enhanced the stability of laccase against temperature, pH, inhibitors and loading time compared with the other cases. The immobilized laccase-mediator system was as efficient as the free enzyme for oxidizing the tested PAHs. After 24h. of incubation, immobilized laccase–HBT showed a system oxidization more than immobilized laccase without (HBT) of PAHs; Chitosan with hyacinth plant and (HBT) resulted better conversion than chitosan with or without HBT. These results indicate a new chance for applying the immobilized laccase in bioremediation.Downloads
References
G. Mastrangelo, E. Fadda, and V. Marzia, "Reviews Polycyclic Aromatic Hydrocarbons and Cancer in Man," vol. 104, no. 11, pp. 1166-1170, 2014.
L. Tissue et al., "Advances in Brief Smoking Increases Carcinogenic Polycyclic Aromatic Hydrocarbons in Human," pp. 6367- 6371, 2001.
K. Liu, W. Han, W. Pan, and J. T. Riley, "Polycyclic aromatic hydrocarbon (PAH) emissions from a coal-fired pilot FBC system," vol. 84, pp. 175-188, 2001.
Z. Zheng and J. P. Obbard, "Oxidation of polycyclic aromatic hydrocarbons (PAH) by the white rot fungus, Phanerochaete chrysosporium," vol. 31, pp. 3-9, 2002.
Y. Z. X. X. M. Tu, J. W. M. Zhang, and W. Y. Z. Y. Y. Shi, "Purification, molecular characterization and reactivity with aromatic compounds of a laccase from basidiomycete Trametes sp. strain AH28-2," pp. 700-701, 2003.
C. Johannes and A. Majcherczyk, "Natural Mediators in the Oxidation of Polycyclic Aromatic Hydrocarbons by Laccase Mediator Systems," vol. 66, no. 2, pp. 524-528, 2000.
N. Duran, N. Durán, M. A. Rosa, and A. D'Annibale, "Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports: a review," Enzyme Microb. Technol., vol. 31, no. 7, pp. 907-931, 2002.
H. Cabana, A. Ahamed, and R. Leduc, "Conjugation of laccase from the white rotfungus Trametes versicolor to chitosan and its utilization for the elimination of triclosan", Biores. Technol, Vol. 102, No. 2, pp. 1656- 1662, 2011.
L. N. Ukiwe, U. U. Egereonu, P. C. Njoku, and C. I. A. Nwoko, "Degradation of Polycyclic Aromatic Hydrocarbons from Soil Leachate by Water Hyacinth ( Eichhornia crassipes )," vol. 4, no. 5, pp. 55-61, 2012.
B. J. S. C. Olson and J. Markwell, "Assays for Determination of Protein Concentration," Curr. Protoc. Pharmacol., vol. 38, no. 1, p. A.3A.1-A.3A.29, 2007.
M. Ibrahim, A.A. Shaltout, M. Soylak, A.F. Jalbout, D.-E. Kamal, Iran Chem. Soc. 6(2009) 364-372
W. Chouyyok, J. Panpranot, C. Thanachayanant and S. Prichanont, J. Mol. Catal. B: Enzym., 2009, 56, 246-252.
F. R. Melgarejo, J. N. R. Lopez, F. G. Canovas and P. A. G. Ruiz,Process Biochem., 2004, 39, 1455-1464.
M. M. Bradford, "A Rapid and Sensitive Method for the Quantitation Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding," vol. 254, pp. 248-254, 1976.
Olson, B. J. & Markwell, J. (2007) Assays for determination of protein concentration, Journal of Current protocols in protein science, 3.4. 1-3.4. 29.
M. Božič, S. Gorgieva, and V. Kokol, "Homogeneous and heterogeneous methods for laccase-mediated functionalization of chitosan by tannic acid and quercetin," Carbohydr. Polym., vol. 89, no. 3, pp. 854-864, 2012.
G. Delanoy, Q. Li, and J. Yu, "Activity and stability of laccase in conjugation with chitosan," vol. 35, pp. 89-95, 2005.
U. N. Dwivedi, P. Singh, V. P. Pandey, and A. Kumar, "Journal of Molecular Catalysis B : Enzymatic Structure - function relationship among bacterial , fungal and plant laccases," "Journal Mol. Catal. B, Enzym., vol. 68, no. 2, pp. 117-128, 2011.
D. Daâssi, S. Rodríguez-Couto, M. Nasri, and T. Mechichi, "Biodegradation of textile dyes by immobilized laccase from Coriolopsis gallica into Ca-alginate beads," Int. Biodeterior. Biodegrad., vol. 90, pp. 71-78,2014.
I. Migneault, C. Dartiguenave, M. J. Bertrand, and K. C. Waldron, "Glutaraldehyde: Behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking," Biotechniques, vol. 37, no. 5, pp. 790-802, 2004.8, 2016.
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