Determination and validation of Tetracycline residues in Poultry by High Performance Liquid Chromatography - Diode Array Detector Technique


  • lobaina M Alrhia Department of Chemistry, Faculty of Science, Tishreen University
  • Issam Mohamad Department of Chemistry, Faculty of Science, Tishreen University
  • sameer mearouf Department of Chemistry, Faculty of Science, Tartous University



Tetracycline, HPLC-DAD, Poultry chest, thigh and liver, Validation of analytical Method.


The aim of this research is separation and determination of trace Tetracycline residues in Poultry chest, thigh and liver using High Performance Liquid Chromatography - Diode Array Detector (HPLC-DAD), with a mobile phase mixture consisting of acetonitrile: methanol: oxalic acid (0.01M) (25:15:60) and chromatographic column C8. The study was done on 32 live poultry individuals. All individuals were injected in the chest by 1m of Tetracycline standard solutions, then slaughter for analysis throughout four successive days. The injection with 10×103 ppb of Tetracycline showed that the traces of Tetracycline residues (according to the Codex Alimentarius Commission) exceeded the value of the maximum residue limit (MRL = 200 ppb) in the thigh and chest meat at the 1st day and the 1st & 2nd days of slay respectively, and exceeded the value of (MRL = 600 ppb) in the liver at the 3rd and 4th days of slay. limit of detection was LOD = 0.451 ppb, limit of quantification LOQ = 1.502 ppb, and recovery percentages of Tetracycline at a concentration of 200.0 ppb for 20 sample Rec.% = (88.966 - 91.055%), (84.623 - 87.667%), (82.198 - 83.688%) for Poultry chest, thigh and liver respectively with a percentage relative standard deviations (RSD%) of < 1 %.


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Original Article

How to Cite

lobaina M. Alrhia, I. Mohamad, and sameer mearouf, “Determination and validation of Tetracycline residues in Poultry by High Performance Liquid Chromatography - Diode Array Detector Technique”, Al-Mustansiriyah Journal of Science, vol. 30, no. 3, pp. 38–46, Oct. 2019, doi: 10.23851/mjs.v30i3.650.

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