Phenotypic and Molecular Characterization of Antimicrobial Resistance and Virulence Determinants in Pseudomonas aeruginosa Isolated from Burn Patients
DOI:
https://doi.org/10.23851/mjs.v37i2.1864Keywords:
Pseudomonas aeruginosa, Burns, Antibiotics, Virulence genes, ESBLAbstract
Background: The pathogen “Pseudomonas aeruginosa (P. aeruginosa)” is extremely hazardous for people with weak immune systems because it has several virulence factors and can resist antibiotics. Objective: To isolate and molecularly identify P. aeruginosa from burn and wound infections and to evaluate its antimicrobial resistance patterns, ESBL production, and virulence characteristics. Methods: A total of 150 burn and wound samples were collected from patients at Erbil West Emergency Hospital and processed immediately. P. aeruginosa was isolated using selective media and confirmed molecularly by PCR targeting rpoB and oprL genes. Virulence genes (nan1, plcH, exoT, toxA, aprA) were detected by PCR. Antimicrobial susceptibility testing was performed against 14 antibiotics using the disk diffusion method. ESBL production was detected using the Double Disc Synergy Test (DDST). Hemolytic and proteolytic activities were evaluated using blood agar and skim milk agar, respectively, while pigment production was assessed on cetrimide agar. Results: Out of 150 samples, 40 P. aeruginosa isolates were identified. Imipenem showed the highest efficacy, while complete resistance was observed against AMP, CTX, P, AMC, and C. Resistance rates were ATM (32.5%), MEM (42.5%), TOB, AK, and CN (45%), CIP (62.5%), CAZ (67.5%), and TE (80%). ESBL production was detected in 26 isolates (65%). β-hemolysis was observed in 25 isolates (62.5%), and strong proteolytic activity was detected in 35 isolates (87.5%). Typical pigment production was observed on cetrimide agar. Conclusions: The study reveals a high prevalence of MDR and ESBL-producing P. aeruginosa with considerable virulence potential in burns and wounds. Imipenem still holds its position as the most promising therapeutic agent; this emphasizes the need for continuous surveillance and proper antibiotic use.
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