The Antibacterial Activity of the Biosynthesized Copper Oxide Nanoparticles by Lantana camara Flowers Extract Against Some Bacterial Isolated from Burns
DOI:
https://doi.org/10.23851/mjs.v33i5.1311Keywords:
Copper oxide nanoparticles, Burns infections, Antibacterial activity, Pathogenic bacteria, Antibiotics susceptibility, Lantana camaraAbstract
The study included 70 Surface swabs collected from patients in different ages of both genders suffering from burns infections. All the specimens were diagnosed by subculturing in differential and selective media, microscopically and by following the biochemical tests to confirm identifications. Antibiotic susceptibility test was done to all the bacterial isolates.The identification of the clinical isolates showed that the 16(22.9%) were belonged to Staphylococcus aureus and 6(8.6%) of Staphylococcus epidermidis , whereas 23(32.8%),20(28.6%) isolates were for Pseudomonas aeruginosa ,Acinetobacter baumannii respectively. Copper oxide nanoparticles were biosynthesized by Lantana camara flower extracts which acted as a reducing agent for copper nitrate salts, characterization of the metal oxide was done by using Ultra violate visible light Spectroscopy, Fourier transforms Infrared spectroscopy, X-ray Diffraction, Atomic Force Microscopy and Field Emission Scanning Electron Microscopy. The biological activity of Copper Oxide Nanoparticles was screened toward three clinical isolates that showed multiple drug resistance isolated from burns by following agar wells diffusion method which proved the ability of copper oxide nanoparticles to inhibit growth of the clinical bacterial isolates. The minimum inhibitory concentrations were determined toward the tested bacteria by using the microdilution method.
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