The Antibacterial Activity of the Biosynthesized Copper Oxide Nanoparticles by Lantana camara Flowers Extract Against Some Bacterial Isolated from Burns
DOI:
https://doi.org/10.23851/mjs.v33i5.1311Keywords:
Copper oxide nanoparticles, Burns infections, Antibacterial activity, Pathogenic bacteria, Antibiotics susceptibility, Lantana camaraAbstract
The study included 70 Surface swabs collected from patients in different ages of both genders suffering from burns infections. All the specimens were diagnosed by subculturing in differential and selective media, microscopically and by following the biochemical tests to confirm identifications. Antibiotic susceptibility test was done to all the bacterial isolates.The identification of the clinical isolates showed that the 16(22.9%) were belonged to Staphylococcus aureus and 6(8.6%) of Staphylococcus epidermidis , whereas 23(32.8%),20(28.6%) isolates were for Pseudomonas aeruginosa ,Acinetobacter baumannii respectively. Copper oxide nanoparticles were biosynthesized by Lantana camara flower extracts which acted as a reducing agent for copper nitrate salts, characterization of the metal oxide was done by using Ultra violate visible light Spectroscopy, Fourier transforms Infrared spectroscopy, X-ray Diffraction, Atomic Force Microscopy and Field Emission Scanning Electron Microscopy. The biological activity of Copper Oxide Nanoparticles was screened toward three clinical isolates that showed multiple drug resistance isolated from burns by following agar wells diffusion method which proved the ability of copper oxide nanoparticles to inhibit growth of the clinical bacterial isolates. The minimum inhibitory concentrations were determined toward the tested bacteria by using the microdilution method.
Downloads
References
J.J.Roth, &W. Hughes , The essential burn unit handbook. CRC Press.2015
D. Church,S. Elsayed,O. Reid, B.Winston and R. Lindsay, Burn wound infections. Clinical microbiology reviews,Vol. 19,No. 2, pp. /403-434, 2006
W.Norbury, D.N.Herndon,J. Tanksley,M.G. Jeschke,C.C. Finnerty, and Scientific Study Committee of the Surgical Infection Society, Infection in burns. Surgical infections, Vol. 17,No.2 , pp./ 250-255 ,2016
I.Khan, K.Saeed, and I. Khan, Nanoparticles: Properties, applications and toxicities. Arabian journal of chemistry, Vol.12,No.7 , pp/ 908-931, 2019
D. Zhang ,X.I. Ma,Y. Gu,H. Huang and G-W.Zhang, Green Synthesis of Metallic Nanoparticles and Their Potential Applications to Treat Cancer. Front. Chem. 8:799, 2020
K. Nithya,P. Yuvasree, N. Neelakandeswari, N. Rajasekaran, K. Uthayarani,M. Chitra, and S.S. Kumar, Preparation and characterization of copper oxide nanoparticles. Int. J. ChemTech Res,Vol. 6, No.3,pp./ 2220-2222, 2014
H.S.Devi, T.D. Singh, (2014). Synthesis of copper oxide nanoparticles by a novel method and its application in the degradation of methyl orange. Adv Electron Electr Eng,Vol. 4, No.1, pp /83-88, 2014
K.H. Hassan,S.K. Saadi, A.A. Jarullah, and P. Harris, Green synthesis and structural characterisation of CuO nanoparticles prepared by using fig leaves extract. Pakistan Journal of Scientific & Industrial Research Series A: Physical Sciences, vol 61, no. 2 ,pp/ 59-65. 2018
F. Ijaz, S. Shahid, S.A. Khan,W. Ahmad, and S. Zaman, Green synthesis of copper oxide nanoparticles using Abutilon indicum leaf extract: Antimicrobial, antioxidant and photocatalytic dye degradation activitie. Tropical Journal of Pharmaceutical Research, vol. 16, no. 4 , pp/ 743-75, 2017
M.I.Din, F. Arshad, A. Rani,A. Aihetasham, M. Mukhtar, and H. Mehmood, Single step green synthesis of stable copper oxide nanoparticles as efficient photo catalyst material. Biomed Mater , vo l. 9, p/ 41,2017
M. Sriramulu, S. Shanmugam, and V.K. Ponnusamy, Agaricus bisporus mediated biosynthesis of copper nanoparticles and its biological effects: An in-vitro study. Colloid andInterfaceScience Communications,35,100254.environmental remediation. Journalofnanobiotechnology, vol. 16, no. 1 ,pp/ 1-24 ,2020
P. Kuppusamy, S. Ilavenil, S. Srigopalram, G.P. Maniam, M.M. Yusoff, N. Govindan, and K. C. Choi, ( Treating of palm oil mill effluent using Commelina nudiflora mediated copper nanoparticles as a novel bio-control agent. Journal of Cleaner Production, vol. 141,pp/ 1023-1029, 2017
M. Luis, M.T. Pezzlo,C.E. Bittencourt, and E. M. Peterson, Color atlas of medical bacteriology. John Wiley & Sons.2020
R. Chowdhury, A. Khan, and M.H. Rashid, Green synthesis of CuO nanoparticles using Lantana camara flower extract and their potential catalytic activity towards the aza-Michael reaction. RSC Advances,vol. 10, no. 24), pp/ 14374-14385,2020
L.L. Duffy, M.J. Osmond-McLeod,J. Judy, and T. King, Investigation into the antibacterial activity of silver, zinc oxide and copper oxide nanoparticles against poultry-relevant isolates of Salmonella and Campylobacter. Food control,vol. 92, pp/ 293-300, 2018
D.D. R Turista, E. Puspitasari, The growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O. Jurnal Teknologi Laboratorium,vol. 8, no. 1, pp/ 1-7, 2019
S. Begum, F. Hasan, S. Hussain, and A. Ali Shah, Prevalence of multi drug resistant Acinetobacter baumannii in the clinical samples from Tertiary Care Hospital in Islamabad, Pakistan. Pakistan journal of medical sciences,vol. 29, no. 5, pp/ 1253-1258, 2013
M. Pastor, M. Moreno-Sastre, A. Esquisabel, E. Sans, M. Viñas, D. Bachiller,Aand J.L. Pedraz, Sodium colistimethate loaded lipid nanocarriers for the treatment of Pseudomonas aeruginosa infections associated with cystic fibrosis. International journal of pharmaceutics, vol. 477, no. 1-2 , 485-494,2014
S.M.M.AL-Dahlaki, Molecular detection and gene expression for hcp and blaOXA-51 genes in Acinetobacter baumannii isolated from different clinical sources. MSC Thesis, College of Science, University of Diyala, Diyala ,Iraq ,p 154, 2020
S.Raut,K.R. Rijal, S. Khatiwada, S. Karna, R. Khanal, J. Adhikari, and B. Adhikari, Trend and characteristics of Acinetobacter baumannii infections in patients attending Universal College of Medical Sciences, Bhairahawa, Western Nepal: a longitudinal study of 2018. Infection and Drug Resistance, 13, 1631,2020
L. Adhikari, K. Roy, D.C. Tsering, R. Pal,andS. Kar, Susceptibility rates of Pseudomonas aeruginosa strains to quinolones. Journal of laboratory physicians, vol. 2,no.02), pp/121-121,2010
F.Nouri, P. Karami,O. Zarei,F. Kosari, M.Y.Alikhani,E. Zandkarimi, E. Rezazadeh Zarandi, and M. Taheri,Prevalence of Common Nosocomial Infections and Evaluation of Antibiotic Resistance Patterns in Patients with Secondary Infections in Hamadan, Iran. Infection and drug resistance, vol. 13, pp/2365-2374,2020
S.B. Almasaudi, Acinetobacter spp. as nosocomial pathogens: Epidemiology and resistance features. Saudi journal of biological sciences, vol.25, no. 3 , pp/586-596.,2018
T.J. Foster, Antibiotic resistance in Staphylococcus aureus.Current status and future prospects. FEMS Microb. Rev.vol. 41, pp/ 430-449,2018
C.Rayner, andW.J. Munckhof,Antibiotics currently used in thetreatment of infections caused by Staphylococcus aureus. Intern. Med. J.35(Suppl 2):S3-16 ,2005.
S. Khoshnood, M. Heidary, A. Asadi,S. Soleimani,M. Motahar,M. Savari, A review on mechanism of action, resistance, synergism, andclinical implications of mupirocin against Staphylococcus aureus. Biomed.Pharmacother. vol.109, pp/1809-1818, 2019
J. C.Andrade, M.F.B. Morais-Braga, G.M. Guedes,S.R. Tintino, M.A. Freitas, I.R.Menezes, andH.D. Coutinho, Enhancement of the antibiotic activity of aminoglycosides by alpha-tocopherol and other cholesterol derivates. Biomedicine & Pharmacotherapy, vol. 68, no. 8, 1065-1069.,2014 pp/
R. Kelmani Chandrakanth,S. Raju, andS.A. Patil, Aminoglycoside-resistance mechanisms in multidrug-resistant Staphylococcus aureus clinical isolates. Current Microbiology, vol .56,no. 6, pp/558-562, 2008
N.E. Holmes,S.Y. Tong, J.S. Davis, S.J. Van Hal, Treatment of methicillin-resistant Staphylococcus aureus: vancomycin and beyond. In Seminars in respiratory and critical care medicine (Vol. 36, No. 01, pp. 017-030). Thieme Medical Publishers. 2015, February).
S.T. Micek, Alternatives to vancomycin for the treatment of methicillin-resistant Staphylococcus aureus infections. Clinical infectious diseases, 45(Supplement_3), S184-S190, 2007
M. Haseeb, M. S. Khan, A. Baker, I. Khan, I. Wahid, M.M. Jaabir, Anticancer and antibacterial potential of MDR Staphylococcus aureus mediated synthesized silver nanoparticles. Biosci. Biotech. Res. Commun, vol. 12,pp/ 26-35.2019
L. Kakoullis, E. Papachristodoulou, P. Chra, and G. Panos, Mechanisms of antibiotic resistance in important gram-positive and gram-negative pathogens and novel antibiotic solutions. Antibiotics, vol. 10, no.4 , p/ 415.2021
M. Asif,I.A. Alvi, I and S.U. Rehman, Insight into Acinetobacter baumannii: pathogenesis, global resistance, mechanisms of resistance, treatment options, and alternative modalities. Infection and drug resistance, vol. 11, p/ 1249., 2018
Z. Pang,R. Raudonis, B.R. Glick, T.J. Lin,Z. Cheng, Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and alternative therapeutic strategies. Biotechnology advances, vol. 37, no.1, pp/177-192,2019
M.M. Swamy and S. BS, Bio-mediated synthesis of ZnO nanoparticles using Lantana Camara flower extract: Its characterizations, photocatalytic, electrochemical and anti-inflammatory applications, 2021
R. Mohammadi-Aloucheh, A. Habibi-Yangjeh, A. Bayrami, S. Latifi-Navid, and A. Asadi, Enhanced anti-bacterial activities of ZnO nanoparticles and ZnO/CuO nanocomposites synthesized using Vaccinium arctostaphylos L. fruit extract. Artificial cells, nanomedicine, and biotechnology, 46(sup1), 1200-1209.
Y.K. Phang,M. Aminuzzaman, M. Akhtaruzzaman, G. Muhammad, S. Ogawa, A. Watanabe, and L.H Tey, Green synthesis and characterization of CuO nanoparticles derived from papaya peel extract for the photocatalytic degradation of palm oil mill effluent (POME). Sustainability,vol. 13,no. 2,p/ 796,2021
N. R. Dhineshbabu, V. Rajendran, N. Nithyavathy, and R. Vetumperumal, Study of structural and optical properties of cupric oxide nanoparticles. Applied Nanoscience,vol. 6, no.6 , pp/933-939,2016
D.Suresh, P.C. Nethravathi, H. Rajanaika, H. Nagabhushana, S.C. Sharma, Green synthesis of multifunctional zinc oxide (ZnO) nanoparticles using Cassia fistula plant extract and their photodegradative, antioxidant and antibacterial activities. Materials Science in Semiconductor Processing,vol. 31,pp/446-454,2015
A.A Radhakrishnan, and B.B. Beena, Structural and optical absorption analysis of CuO nanoparticles. Indian J. Adv. Chem. Sci,vol. 2, no.2,pp/ 158-161, 2020
A. Mokhtari, A. Goudarzi, M. Benam, S.M. Langroodi, S. Karimmohammad, and M. Keyvanfard, Fabrication and characterization of Cu (OH) 2/CuO nanowires as a novel sensitivity enhancer of the luminol-H2O2 chemiluminescence system: determination of cysteine in human plasma. RSC Advances,vol. 6, no. 7 , pp/5320-5329,2016
D. Das, B.C. Nath, P. Phukon, and S.K. Dolui, Synthesis and evaluation of antioxidant and antibacterial behavior of CuO nanoparticles. Colloids and Surfaces B: Biointerfaces, vol. 101,pp/ 430-433,2013
A. Moores,and F. Goettmann, The plasmon band in noble metal nanoparticles: an introduction to theory and applications. New Journal of Chemistry,vol. 30,no.8 , pp/ 1121-1132, 2006
N.A. Thamer, N. Q. Muftin, and S. H. N. Al-Rubae, Optimization properties and characterization of green synthesis of copper oxide nanoparticles using aqueous extract of Cordia myxa L. leaves. Asian J. Chem, vol. 30, no. 7 , pp/1559-1563,2018
M. Ahamed, H.A. Alhadlaq, M.A. Khan, P. Karuppiah, N.A. Al-Dhabi, Synthesis, characterization, and antimicrobial activity of copper oxide nanoparticles. Journal of Nanomaterials, 2014.
M.Y. Bhat, M.Z. Gul,L.R. Lohamror,I.A. Qureshi, and I.A. Ghazi, An in vitro study of the antioxidant and antiproliferative Properties of Artemisia absinthium-A Potent Medicinal Plant. Free Radicals and Antioxidants, vol.8, no. 1, pp/ 18-25, 2018
A. Mansoori, N. Singh, S.K. Dubey, T.K Thakur, N. Alkan, S.N. and A. Kumar, Phytochemical characterization and assessment of crude extracts from Lantana camara L. for antioxidant and antimicrobial activity. Frontiers in Agronomy, 2, 58226,2020
A.M. AL- tameemi , Preparation and characterization of some metals oxides nanoparticles and study their biological activity. M.Sc.thesis . College of Education for Pure Sciences . University of Diyala,diyala ,iraq ,2019
K.H. Hassan, and E.R. Mahdi, Synthesis and characterization of copper, iron oxide nanoparticles used to remove lead from aquous solution. Asian journal of applied sciences,vol. 4, no. 3, 2016
J.H. Taha, N. K. Abbas,and A.A. Al-Attraqchi, Green Synthesis and Evaluation of Copper Oxide Nanoparticles using Fig Leaves and their Antifungal and Antibacterial Activities. Int. J. Drug Delivery Technol., 10,pp/ 378-382, 2020
U. Halder, R.K. Roy, R. Biswas, D. Khan, K. Mazumder, and R.Bandopadhyay, Synthesis of copper oxide nanoparticles using capsular polymeric substances produced by Bacillus altitudinis and investigation of its efficacy to kill pathogenic Pseudomonas aeruginosa. Chemical Engineering Journal Advances, 11, 100294, 2022
M. L. Ermini, and V. Voliani, Antimicrobial nano-agents: The copper age. ACS nano,vol. 15,no.4 ,pp/ 6008-6029,2021
S,A. Akintelu , A.S.Folorunso, F.A. Folorunso, F. A., and A.K. Oyebamiji, Green synthesis of copper oxide nanoparticles for biomedical application and environmental remediation. Heliyon, vol.6, no. 7 , e04508,2020
J.M. Andrews, Determination of minimum inhibitory concentrations. Journal of antimicrobial Chemotherapy, vol. 48 ,no. 1 , pp/ 5-16,2001
R. Dadi, R. Azouani, M. Traore, C. Mielcarek, and A. Kanaev, Antibacterial activity of ZnO and CuO nanoparticles against gram positive and gram negative strains. Materials Science and Engineering: C, 104, 109968, 2019
G. Ungur, and J. Hrůza, Influence of copper oxide on the formation of polyurethane nanofibers via electro spinning. Fibers and Polymers, vol. 16, no.3 , 621-628,2015
R. Goyal, L. K. Macri, H.M. Kaplan, and J. Kohn, Nanoparticles and nanofibers for topical drug delivery. Journal of Controlled Release,vol. 240, pp/ 77-92,2016
Downloads
Key Dates
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
(Starting May 5, 2024) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution (CC-BY) 4.0 License that allows others to share the work with an acknowledgement of the work’s authorship and initial publication in this journal.
How to Cite
