Determination of some virulence factors of Candida spp. isolated from locally produced cheese in Diyala Governorate-Iraq

Authors

  • Suhail Jawdat Fadihl Department of Pathological Analysis, Baquba Technical Institute, Middle Technical University.

DOI:

https://doi.org/10.23851/mjs.v27i4.13

Keywords:

Yeast isolation, chesses, Candida albicans, virulence factors.

Abstract

Locally produced cheese which called (Gibin Al arab) is one of the most common dairy products in Iraq, it has an economic importance and great social value. This research aimed to identify yeast species from locally produced cheese (Gibin Al Arab) in Diyala city which traditionally made and sold in markets of old town in Baquba, and study some of virulence factors (Esterase production, Phospholipase and Hemolytic production) of yeasts belong to genus of Candida . All cheese samples showed contamination with varying number of yeast, total 88 yeast isolates obtained from 70 cheese samples, they were Geotrichum candidum(20.5%), Rhodotorela species(19.4%), Candida parapsilosis (18%), Candida albicans (13.6%), Candida  tropicalis (10.5%), Candida krusei (8%), Saccharomyces cerevisice (3.3%) and mixed yeast (un identified) at rate of (6.7%). Species of Candida formed half of the total isolates and the most prevalent isolate of Candida spp. was Candida parapsilosis .According to the results determining of  (Esterase production, Phospholipase and Hemolytic production) as a virulence factors identifying Candida spp. these activities referred that all isolates of Candida spp. show one or more of these activities and that isolates of  medically important species Candida albicans were the most virulent isolates. this referred to the importance of take attention about consuming of such types of dairy products and need for applying more hygienic measures during handling, processing of milk and form of storage and/or selling of cheese.

Downloads

Download data is not yet available.

References

Sharoud WM EL, Belloch C, Peris D, and Querol A. Molecular Identification of Yeasts Associated with Traditional Egyptian Dairy Products. Journal of Food Science. 1750-3841.2009

Fleet GH. Yeasts in foods and beverages: impact on product quality and safety. Curr Opin Biotechnol. 18:170–5. 2007.

El-Sharoud WM. Prevalence and survival of Campylobacter in Egyptian dairy products. Food Res. Int. 42:622–26. 2009.

El-Sharoud WM, Spano G. Diversity and enterotoxigenicity of Staphylococcus spp. associated with Domiati cheese. J Food Prot.71:2567–71. 2008.

El-Baradei G, Agnes DB and Ogier JC. Biodiversity of bacterial ecosystems in traditional Egyptian Domiati cheese. Appl Environ Microbiol.73:1248–55. 2007.

Soliman Neveen SM and Aly SA. Occurrence and identification of yeast species isolated from Egyptian Karish cheese. Journal of Yeast and Fungal Research. 2(4):59-64. 2011.

Devoyod JJ . Yeasts in cheese making. In: Spencer, F.J., Spencer. Yeast Technology. 2008; SpringerVerlag, Berlin: 228–245.

Wanderley L, Bianchin A, Paz Arruda Teo CR, and Fuentefria AM. Occurrence and pathogenicity of Candida spp. in unpasteurized cheese . Brazilian Journal of Biosciences. 11 (2):145-148. 2013.

Melville PA, Benites NR, Ruz-Peres M and Yokoya E. Proteinase and phospholipase activities and development at different temperatures of yeasts isolated from bovine milk. Journal of Dairy Research. 78: 385-390. 2011.

Lima CC, Lima LA, Cerqueira MP, Ferreira EG and Rosa CA . Bacterias do acido lactico e

leveduras associadas com o queijo-de-minas artesanal produzido na região da Serra do Salitre. Arquivos Brasileiros de Medicina Veterinária e Zootecnia. 2009; 61: 266-272.

Santos RC and Marin J M. Isolation of Candida spp. from mastitic bovine milk in Brazil. Mycopathologi. 159: 251-253. 2005.

Sardi J, Scorzoni L, Bernardi T, Fusco- Almeida A and Mendes Giannini M. Candida species: current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. J Med Microbiol.62:10-24. 2013.

Sachin CD, Ruchi K and Santosh S. In-vitro evalution of proteinase, phospholipase and haemolysin activities of Candida species isolated from clinical specimens. Int J Med Biomed Res.1:153-157. 2012.

Aher CS. Species distribution, virulence factors and antifungal susceptibility profile of Candida isolated from Oropharyngeal lesions of HIV infected patients. Int.J.Curr. Microbiol.App.Sci.3 (1):453460. 2014.

Yousef A. Effect of technological factors on the manufacture of karish cheese. Egyptian J. Dairy Sci. 31(1): 161-165. 2007.

Dawood M, Abdou R and Salem G. Effect of irradiation of skim milk on the quality of karish cheese. Egyptian J. Dairy Sci.32(4): 500-509. 2009.

Van der Walt and Yarrow D. Methods for the isolation, manteinance, classification and identification of yeasts. In: Kregervan Rij, N.J.W. Ed.., The Yeasts: A Taxonomic Study. 3rd edn. Elsevier, Amsterdam, p. 45–104. 2009.

Kurtzman CP. Molecular taxonomy of the yeast. Yeast.10: p:1720-1727. 1994.

Cruickshan R, JP Duguid, BP Marmion and RHA Swain. Medical microbiology. 12 edit. th Vol. 2 Churchill Livingstone, Edinburgh, London and New York. 1975.

Tirunarayana MO and Lundbeek H. Investigations on the enzymes and toxins of staphylococci: assay of lipase using Tween as the substrate. Acta pathol.Microbiol.Scand. 72:263-433. 1968.

Brocklehurst TF, Lund BM. Microbiological changes in cottage cheese varieties during storage at 7 C. Food Microbiol. 2(3): 207– 233.1985.

Bulhões CCC and Rossi Junior OD. Ocorrência de bactérias do gênero Aeromonas em queijo-deminas frescal artesanal. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 54(3): 320-24. 2002.

Luo G and Samaranayake LP Yan JYC. Candida species exhibit differential in vitro hemolytic activities. J Clin Microbiol, 39:2971-2974. 2000.

Loretan T, Viljoen BC, Mostert JF, Vogel AM and Jordaan HF. A preliminary study of the diversity and technological properties of indigenous traditional South African fermented milk. F47; Sept 2–3; Brussels, Belgium. p 178–82.1998.

Viljoen B. Yeast ecological interactions. In: Querol A, Fleet GH, editors. Yeasts in food and beverages. Germany: Springer. p 83–110. 2006.

Refa M, AH Gobba and H Rieth. Monograph on yeast diagnosis, disease and treatment. Egypt. Vet. Med. J.16: 255-315. 1969.

Hakim AS, Abuelnaga ASM and Sayed El Ahl RMH. Isolation, Biochemical Identification and Molecular Detection of Yeasts from Kareish Cheese. International Journal of Microbiological Research. 4(1):95-100. 2013.

Viljoen BC, Dykes GA, Callis M and von Holy A. Yeasts associated with cheese packaging. Int. J. Food Microbiol.42(1) 53–62. 2010.

Borelli Beatriz M, Ferreira Elaine G, Lacerda Inayara CA and Franco Glo´ria R. Yeast populations associated with the artisanal cheese produced in the region of Serra da Canastra, Brazil. World J Microbiol Biotechnol. 22:1115–1119. 2006.

Hidalgo H. Candidiases. (International Committee on Microbiological Specifications for Foods, ICMSF, (2005). Microorganisms in food, their significance and methods of enumeration) .2nd Ed. Academic press, London. Interna. Med. J. 88 (1) 52-58. 2011.

Seider K, Heyken A, Lü ttich A, Miramon P and Hube B . Interaction of pathogenic yeasts with phagocytes: survival, persistence and escape . Current Opinion in Microbiology. 13: 392– 400. 2010.

Aponte M, Pepe O and Blaiotta G. Short communication:Identification and technological characterization of yeast strains isolated from samples of cottage cheese. J. Dairy Sci.93(6): 23582361. 2010.

Aktas E, Yigit N and Ayyildiz A. Esterase Activity in Various Candida Species. The Journal of

International Medical Research. 2002; 30: 322 - 324.

Liang Yang Y. Virulance factors of Candida species. J Microbial Immunol Infect. 36:223-228. 2003.

Negri M, Faria MG, Guilhermetti E, Alves AA, Paula CR, and Svidzinski TIE. Hemolytic activity and production of germ tubes related to pathogenic potential of clinical isolates of Candida albicans. Journal of Basic and Applied Pharmaceutical Sciences.31(1):89-93. 2010.

Sachin CD , Ruchi K, and Santosh S. In vitro evaluation of proteinase, phospholipase and haemolysin activities of Candida species isolated from clinical specimens. International Journal of Medicine and Biomedical Research. 1(2): 153-157. 2012.

Downloads

Key Dates

Published

01-03-2017

Issue

Section

Original Article

How to Cite

[1]
S. J. Fadihl, “Determination of some virulence factors of Candida spp. isolated from locally produced cheese in Diyala Governorate-Iraq”, Al-Mustansiriyah Journal of Science, vol. 27, no. 4, Mar. 2017, doi: 10.23851/mjs.v27i4.13.

Similar Articles

1-10 of 52

You may also start an advanced similarity search for this article.