Correlation Between the Estimated and the Measured Serum Apolipoprotein –B100 in Kurd Subjects: A Trend to Establish a New Formula


  • Hawri Fatih Sami Department of Medical Microbiology, Cihan University-Erbil, Erbil, Kurdistan Region, IRAQ.
  • Kadhim Qadir S. Hussein Sulaimani Polytechnic University, Sulaimani Technical Institute, Department of Pharmacy, Kurdistan Region, Sulaimani, Iraq.
  • Mudhafar Mohamed M. Saeed Department of Pharmacy, Sulaimani Technical Institute, Sulaimani Polytechnic University, Sulaimani, Kurdistan Region, IRAQ.
  • Hasan Qader Sofihussein Department of Pharmacy, Sulaimani Technical Institute, Sulaimani Polytechnic University, Sulaimani, Kurdistan Region, IRAQ.
  • Abdulqader Azeez Al-Naqshbandi College of Pharmacy, Hawler Medical University, Erbil, Kurdistan Region, IRAQ.



Apolipoprotein B100, Gender, Kurd ethnicity, Estimated equation


Apolipoprotein B100 (ApoB100) provides a good assessment of atherogenic lipoproteins (very low, intermediate, and low-density lipoproteins (VLDL, IDL, and LDL)). There is evidence that polymorphism of ApoB100 was observed in many conditions and it links with obesity, diabetes mellitus hypertension, and chronic inflammation, which could be related to the broad field of the atherothrombotic process, and could be one of the leading causes of coronary artery disease (CAD). It can be computed using a formula that makes use of a measurement of non-high-density lipoprotein levels. This study aimed to derive an estimated equation of ApoB100 from the measured ApoB100 levels specific to the healthy subjects of Kurd race/ethnicity in the Kurdistan region of Iraq taking into consideration the gender-based effect and the status of fasting and postprandial effects. A total number of 45 healthy subjects (23 males and 22 females) were enrolled in the study. The following measurements were achieved: anthropometric indices, blood pressure, lipid profile, including ApoB100, and blood sugar. The biochemical measurements were carried out at fasting and postprandial states. Specific equations were derived for calculating the levels of ApoB100. Significant differences in the anthropometric indices, blood pressure, and lipid profile were observed between males and females. The calculated ApoB100 levels were significantly less than the measured ApoB100 levels in both genders and fasting and postprandial states. The estimated equations for ApoB100 for females have differed from that for males at fasting and postprandial states. The levels of ApoB100 can be determined instead of measuring it in the laboratory by using a specific equation for healthy Kurd people. These equations are gender and race/ethnicity based. The established equation of estimated ApoB100 levels in males differed from that in females which is attributed to the cardio-metabolic factors and higher levels of systolic and mean arterial blood pressures among males. Apo B level is a quantitative index of plasma atherogenic lipids in hypertensive patients who presented with dyslipidemia.


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Zaizafoon N. Nasif, Emad M. Eltayef, Nasser M.Al-Janabi, Ali N. Niseaf. Relation of obesity to serum ferritin, lipid profile, uric acid and urea at Obesity Medical Center in Iraq. Al-Mustansiriyah Journal of Science 2018; 28(1):87-96.


Sniderman AD, St-Pierre AC, Cantin B, Dagenais GR, Després JP, Lamarche B, et al. Concordance/discordance between plasma apolipoprotein B levels and the cholesterol indexes of atherosclerotic risk. Am J Cardiol 2003;91(10):1173-7.

CrossRef | PubMed

Levinson SS. Comparison of apolipoprotein B and non-high-density lipoprotein cholesterol for identifying coronary artery disease risk based on receiver operating curve analysis. Am J ClinPathol2007;127(3):449-55.

CrossRef | PubMed

Nordestgaard BG, Langsted A, Mora S, Kolovou G, Baum H, Bruckert E et al. Fasting is not routinely required for determination of a lipid profile: clinical and laboratory implications including flagging at desirable concentration cut points-A Joint Consensus Statement from the European Atherosclerosis Society and European Federation of Clinical Chemistry and Laboratory Medicine. Eur Heart Journal 2016; 37(25):1944-58.

CrossRef | PubMed

Feingold KR, Grunfeld C. Utility of Advances Lipoprotein Testing in Clinical Practice. In: De Groot LJ, Beck-Peccoz P, Chrousos G, Dungan K, Grossman A, Hershman JM et al, (eds.) Endotext [Internet]. South Dartmouth (MA):, Inc.; 2000-.2016 Mar 24.

[6] Hermans MP, Sacks FM, Ahn SA, Rousseau MF. Non-HDL-cholesterol as a valid surrogate to apolipoprotein B100 measurement in diabetes: Discriminant Ratio and unbiased equivalence. CardiovascDiabetol 2011.

CrossRef | PubMed

Hochner H, Allard C, Granot-Hershkovitz E, Chen J, Sitlani CM, Sazdovska S, et al. Parent-of-origin effects of the APOB gene on adiposity in young adults.PLoS Genet 2015;11(10).

CrossRef | PubMed

Zhang X, Zhang W, Saraf SL, Nouraie M, Han J, Gowhari M, et al.. Genetic polymorphism of APOB is associated with diabetes mellitus in sickle cell disease Hum Genet 2015;134(8):895-904.

CrossRef | PubMed

Ríos-González BE, Ibarra-Cortés B, Ramírez-López G, Sánchez-Corona J, Magaña-Torres MT. Association of polymorphisms of genes involved in lipid metabolism with blood pressure and lipid values in Mexican hypertensive individuals. Dis Markers 2014.

CrossRef | PubMed

Zhuanglin Zeng, Bingxin Cao, Xiaopeng Guo, Weijuan Li, Songhai Li, Juan Chen, et al. Apolipoprotein B-100 Peptide 210 antibody inhibits atherosclerosis by regulation of macrophage that phagocytizes oxidized lipid. Am J Transl Res. 2018; 10(6): 1817-1828.

Ahmed Abduljalal Abduljabbar, Parween Abdulsamad Ismail, Chalak Abdulsamad Ismail. Investigation of Malondialdehyde (MDA), C reactive protein (CRP) and Homocysteine in sera of patients with Angina Pectoris. Al-Mustansiriyah Journal of Science 2018; 30(1):68-74.


Tsimikas S, Clopton P, Brilakis ES, Marcovina SM, Khera A, Miller ER, et al. Relationship of oxidized phospholipids on apolipoprotein B-100 particles to race/ethnicity, apolipoprotein(a) isoform size, and cardiovascular risk factors: results from the Dallas Heart Study. Circulation 2009;119(13):1711-9.

CrossRef | PubMed

Jasmeen Chahal, Sunita Gupta, Sumit Pal Singh Chawal, Harpreet Grewal. Comparative study on fasting and postprandial lipid profile in type 2 diabetes mellitus. J Family Med Prim Care 2021;10(3): 1288-1293.

CrossRef | PubMed

Fedacko J, Pella D, Jarcuska P, Siegfried L, Janicko M, Veselíny E, et al. Clinical and biochemical determinants of metabolic syndrome among Roma and non-Roma subjects in the eastern part of Slovakia. Cent Eur J Public Health.2014;22 Suppl: S75-80.

CrossRef | PubMed

Panagiotakos DB, Pitsavos C, Skoumas Y, Lentzas Y, Papadimitriou L, Chrysohoou C, et al. Abdominal obesity, blood glucose and apolipoprotein B levels are the best predictors of the incidence of hypercholesterolemia (2001-2006) among healthy adults: the ATTICA Study. Lipids in Health and Disease, 2008.

CrossRef | PubMed

Jennifer Behbodikhah, Saba Ahmed, Ailin Elyasi, Lora J. Kasselman, Joshua De Leon, Amy D. Glass, et al. Apolipoprotein B and Cardiovascular Disease: Biomarkers and Potential Therapeutic Target. Metabolites 2021.

CrossRef | PubMed

Islam S, Gutin B, Manos T, Smith C, Treiber F. Low-density lipoprotein cholesterol/apolipoprotein B-100 ratio: interaction of family history of premature atherosclerotic coronary artery disease with race and gender in 7 to 11-year-olds. Paediatrics 1994.

CrossRef | PubMed

Serban C, Susan L, Mateescu R, NoveanuL, Pacurari A, Caraba A, et al. Apolipoprotein B and standard lipid profile in hypertensive patients with atherogenic dyslipidemia. J Exp Med Surg Res 2010:2: 98-103.

Otokozawa S, Ai M, Diffenderfer MR, Asztalos BF, Tanaka A, Lamon-Fava S, et al. Fasting and postprandial apolipoprotein B-48 levels in healthy, obese, and hyperlipidemic subjects. Metabolism 2009;58(11):1436-1542.

CrossRef | PubMed




How to Cite

H. F. Sami, K. Q. S. . Hussein, M. M. M. . Saeed, H. Q. Sofihussein, and A. A. . Al-Naqshbandi, “Correlation Between the Estimated and the Measured Serum Apolipoprotein –B100 in Kurd Subjects: A Trend to Establish a New Formula”, Al-Mustansiriyah Journal of Science, vol. 34, no. 1, pp. 45–51, Mar. 2023.



Chemical Science