Detection of gyrA and parC Genes in Clinical Acinetobacter Baumannii Isolates

Authors

  • Zahraa Abd AL-Kareem Kadham Department of Biology, College of Science, Mustansiriyah University, Baghdad, IRAQ.

DOI:

https://doi.org/10.23851/mjs.v33i4.1188

Keywords:

Acinetobacter baumannii, PCR, gyrA, parC, fuoroquinolone resistance

Abstract

100 isolates of Acinetobacter baumannii were collected from different clinical sources including (blood, sputum and burns) from hospitals in Baghdad - Iraq. In order to investigate its resistance to fluoroquinolones. MIC assay for ciprofloxacin was performed using E-test, and PCR assay for gry A and parC genes. The results of the MIC showed that A. baumannii was sensitive to ciprofloxacin at concentration=<4 μg/ml. As for the PCR assay, the prevalence of gyr A gene in 40 of the isolates was 40%, while the par C gene in 16 of the isolates was 16%. This research shed light on the rapid spread of fluoroquinolone resistance that included both ciprofloxacin and levofloxacin among A.baumannii bacteria isolated from clinical sources.

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Key Dates

Published

30-12-2022

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Section

Original Article

How to Cite

[1]
Z. A. A.-K. . Kadham, “Detection of gyrA and parC Genes in Clinical Acinetobacter Baumannii Isolates”, Al-Mustansiriyah J. Sci., vol. 33, no. 4, pp. 57–62, Dec. 2022, doi: 10.23851/mjs.v33i4.1188.

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