Detection of gyrA and parC Genes in Clinical Acinetobacter Baumannii Isolates
Keywords:Acinetobacter baumannii, PCR, gyrA, parC, fuoroquinolone resistance
100 isolates of Acinetobacter baumannii were collected from different clinical sources including (blood, sputum and burns) from hospitals in Baghdad - Iraq. In order to investigate its resistance to fluoroquinolones. MIC assay for ciprofloxacin was performed using E-test, and PCR assay for gry A and parC genes. The results of the MIC showed that A. baumannii was sensitive to ciprofloxacin at concentration=<4 μg/ml. As for the PCR assay, the prevalence of gyr A gene in 40 of the isolates was 40%, while the par C gene in 16 of the isolates was 16%. This research shed light on the rapid spread of fluoroquinolone resistance that included both ciprofloxacin and levofloxacin among A.baumannii bacteria isolated from clinical sources.
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