Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant

Authors

  • Ali A. Taha Applied Science Department, Biotechnology Division, University of Technology.
  • Nahida J. Hameed Applied Science Department, Materials Division, University of Technology.
  • Farah Hamed Ali Applied Science Department, Materials Division, University of Technology.

DOI:

https://doi.org/10.23851/mjs.v31i3.670

Keywords:

Polycyclic aromatic hydrocarbons PAH, Immobilized laccase, Trametes versicolor, Bioremediation, HBT, Conversation, Hyacinth plant.

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are recognized as a toxic, mutagenic and/or carcinogenic compounds, and their pollution of soil and aquifer is of increasing environmentally risk. Laccases (E.C. 1.10.3.2) are phenoloxidases catalyze the oxidation of PAHs in the presence of a mediator compound and hyacinth plant. In this study laccase from Trametes versicolor was immobilized into chitosan, and the potential to oxidize anthracene in the presence of 1-hydroxybenzotriazole (HBT) was examined. Results indicated that the immobilization enhanced the stability of laccase against temperature, pH, inhibitors and loading time compared with the other cases. The immobilized laccase-mediator system was as efficient as the free enzyme for oxidizing the tested PAHs. After 24h. of incubation, immobilized laccase–HBT showed a system oxidization more than immobilized laccase without (HBT) of PAHs; Chitosan with hyacinth plant and (HBT) resulted better conversion than chitosan with or without HBT. These results indicate a new chance for applying the immobilized laccase in bioremediation.

Author Biography

  • Farah Hamed Ali, Applied Science Department, Materials Division, University of Technology.
    At present, I am a Phd student at the University of Technology.

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Published

20-08-2020

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Section

Chemical Science

How to Cite

[1]
A. A. Taha, N. J. Hameed, and F. H. Ali, “Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant”, Al-Mustansiriyah Journal of Science, vol. 31, no. 3, pp. 14–20, Aug. 2020, doi: 10.23851/mjs.v31i3.670.

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