Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant

Authors

  • Ali A. Taha Applied Science Department, Biotechnology Division, University of Technology.
  • Nahida J. Hameed Applied Science Department, Materials Division, University of Technology.
  • Farah Hamed Ali Applied Science Department, Materials Division, University of Technology.

DOI:

https://doi.org/10.23851/mjs.v31i3.670

Keywords:

Polycyclic aromatic hydrocarbons PAH, Immobilized laccase, Trametes versicolor, Bioremediation, HBT, Conversation, Hyacinth plant.

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are recognized as a toxic, mutagenic and/or carcinogenic compounds, and their pollution of soil and aquifer is of increasing environmentally risk. Laccases (E.C. 1.10.3.2) are phenoloxidases catalyze the oxidation of PAHs in the presence of a mediator compound and hyacinth plant. In this study laccase from Trametes versicolor was immobilized into chitosan, and the potential to oxidize anthracene in the presence of 1-hydroxybenzotriazole (HBT) was examined. Results indicated that the immobilization enhanced the stability of laccase against temperature, pH, inhibitors and loading time compared with the other cases. The immobilized laccase-mediator system was as efficient as the free enzyme for oxidizing the tested PAHs. After 24h. of incubation, immobilized laccase–HBT showed a system oxidization more than immobilized laccase without (HBT) of PAHs; Chitosan with hyacinth plant and (HBT) resulted better conversion than chitosan with or without HBT. These results indicate a new chance for applying the immobilized laccase in bioremediation.

Author Biography

Farah Hamed Ali, Applied Science Department, Materials Division, University of Technology.

At present, I am a Phd student at the University of Technology.

References

G. Mastrangelo, E. Fadda, and V. Marzia, "Reviews Polycyclic Aromatic Hydrocarbons and Cancer in Man," vol. 104, no. 11, pp. 1166-1170, 2014. DOI: https://doi.org/10.1289/ehp.961041166

CrossRef | PubMed

L. Tissue et al., "Advances in Brief Smoking Increases Carcinogenic Polycyclic Aromatic Hydrocarbons in Human," pp. 6367- 6371, 2001.

K. Liu, W. Han, W. Pan, and J. T. Riley, "Polycyclic aromatic hydrocarbon (PAH) emissions from a coal-fired pilot FBC system," vol. 84, pp. 175-188, 2001.

CrossRef DOI: https://doi.org/10.1016/S0304-3894(01)00196-0

Z. Zheng and J. P. Obbard, "Oxidation of polycyclic aromatic hydrocarbons (PAH) by the white rot fungus, Phanerochaete chrysosporium," vol. 31, pp. 3-9, 2002.

CrossRef DOI: https://doi.org/10.1016/S0141-0229(02)00091-1

Y. Z. X. X. M. Tu, J. W. M. Zhang, and W. Y. Z. Y. Y. Shi, "Purification, molecular characterization and reactivity with aromatic compounds of a laccase from basidiomycete Trametes sp. strain AH28-2," pp. 700-701, 2003. DOI: https://doi.org/10.1007/s00253-002-1169-3

CrossRef | PubMed

C. Johannes and A. Majcherczyk, "Natural Mediators in the Oxidation of Polycyclic Aromatic Hydrocarbons by Laccase Mediator Systems," vol. 66, no. 2, pp. 524-528, 2000. DOI: https://doi.org/10.1128/AEM.66.2.524-528.2000

CrossRef | PubMed

N. Duran, N. Durán, M. A. Rosa, and A. D'Annibale, "Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports: a review," Enzyme Microb. Technol., vol. 31, no. 7, pp. 907-931, 2002.

CrossRef DOI: https://doi.org/10.1016/S0141-0229(02)00214-4

H. Cabana, A. Ahamed, and R. Leduc, "Conjugation of laccase from the white rotfungus Trametes versicolor to chitosan and its utilization for the elimination of triclosan", Biores. Technol, Vol. 102, No. 2, pp. 1656- 1662, 2011. DOI: https://doi.org/10.1016/j.biortech.2010.09.080

CrossRef | PubMed

L. N. Ukiwe, U. U. Egereonu, P. C. Njoku, and C. I. A. Nwoko, "Degradation of Polycyclic Aromatic Hydrocarbons from Soil Leachate by Water Hyacinth ( Eichhornia crassipes )," vol. 4, no. 5, pp. 55-61, 2012.

CrossRef DOI: https://doi.org/10.5539/ijc.v4n5p55

B. J. S. C. Olson and J. Markwell, "Assays for Determination of Protein Concentration," Curr. Protoc. Pharmacol., vol. 38, no. 1, p. A.3A.1-A.3A.29, 2007. DOI: https://doi.org/10.1002/0471141755.pha03as38

CrossRef | PubMed

M. Ibrahim, A.A. Shaltout, M. Soylak, A.F. Jalbout, D.-E. Kamal, Iran Chem. Soc. 6(2009) 364-372

CrossRef DOI: https://doi.org/10.1007/BF03245846

W. Chouyyok, J. Panpranot, C. Thanachayanant and S. Prichanont, J. Mol. Catal. B: Enzym., 2009, 56, 246-252.

CrossRef DOI: https://doi.org/10.1016/j.molcatb.2008.05.009

F. R. Melgarejo, J. N. R. Lopez, F. G. Canovas and P. A. G. Ruiz,Process Biochem., 2004, 39, 1455-1464.

CrossRef DOI: https://doi.org/10.1016/S0032-9592(03)00276-0

M. M. Bradford, "A Rapid and Sensitive Method for the Quantitation Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding," vol. 254, pp. 248-254, 1976.

CrossRef DOI: https://doi.org/10.1016/0003-2697(76)90527-3

Olson, B. J. & Markwell, J. (2007) Assays for determination of protein concentration, Journal of Current protocols in protein science, 3.4. 1-3.4. 29. DOI: https://doi.org/10.1002/0471140864.ps0304s48

CrossRef | PubMed

M. Božič, S. Gorgieva, and V. Kokol, "Homogeneous and heterogeneous methods for laccase-mediated functionalization of chitosan by tannic acid and quercetin," Carbohydr. Polym., vol. 89, no. 3, pp. 854-864, 2012. DOI: https://doi.org/10.1016/j.carbpol.2012.04.021

CrossRef | PubMed

G. Delanoy, Q. Li, and J. Yu, "Activity and stability of laccase in conjugation with chitosan," vol. 35, pp. 89-95, 2005. DOI: https://doi.org/10.1016/j.ijbiomac.2005.01.003

CrossRef | PubMed

U. N. Dwivedi, P. Singh, V. P. Pandey, and A. Kumar, "Journal of Molecular Catalysis B : Enzymatic Structure - function relationship among bacterial , fungal and plant laccases," "Journal Mol. Catal. B, Enzym., vol. 68, no. 2, pp. 117-128, 2011.

CrossRef DOI: https://doi.org/10.1016/j.molcatb.2010.11.002

D. Daâssi, S. Rodríguez-Couto, M. Nasri, and T. Mechichi, "Biodegradation of textile dyes by immobilized laccase from Coriolopsis gallica into Ca-alginate beads," Int. Biodeterior. Biodegrad., vol. 90, pp. 71-78,2014.

CrossRef DOI: https://doi.org/10.1016/j.ibiod.2014.02.006

I. Migneault, C. Dartiguenave, M. J. Bertrand, and K. C. Waldron, "Glutaraldehyde: Behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking," Biotechniques, vol. 37, no. 5, pp. 790-802, 2004.8, 2016. DOI: https://doi.org/10.2144/04375RV01

CrossRef | PubMed

Downloads

Published

2020-08-20

How to Cite

[1]
A. A. Taha, N. J. Hameed, and F. H. Ali, “Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant”, MJS, vol. 31, no. 3, pp. 14–20, Aug. 2020.

Issue

Section

Chemical Science