Bacterial Pathogens of Patients with Urinary Tract  Infections  and Antibiotics Susceptibility in Taiz Governorate-Yemen

ahmed sallem alganady

doi:10.23851/mjs.v30i1.530

Authors

ahmed sallem alganady Department of Medical laboratory technology, Al-Ma’afer Community College, Taiz, Yemen

DOI:

https://doi.org/10.23851/mjs.v30i1.530

Keywords:

Urinary tract infections, Bacterial pathogens, Antibiotic Susceptibility, Taiz Governorate, Yemen

Abstract

One of the most prevalent bacterial infections are urinary tract infections (UTIs), which affect any age of individuals and they appear higher in women than men, the major problem regarding UTIs are the difficult of antibiotics treatment, due to bacterial pathogens resistance. So, the current study aimed to detect the types of bacterial pathogens and their antibiotics susceptibility of UTIs in Taiz Governorate – Yemen. In this cohort study, one hundred and ten (110) mid-stream urine samples were collected from a symptomatic UTIs patient, the type of bacterial pathogens was identified by using standard bacteriological media and biochemical tests and determine the antibiotics sensitivity to these bacteria using disk diffusion tests. Out of one hundred and ten of urine samples:: 65 of patients were females and 45 were males and the bacterial growth in these patients were sixty-four [46(58.2%)] : (33.7%) were females and (24.5%) were males. Our findings showed that the types of bacterial pathogens include: Escherichia coli (32.7%), Klebsiellapneumoniae (9.1%), Staphylococcus aureus (6.4%), Proteus mirabilis (5.5%) and Pseudomonas aeruginosa (4.5%). The majority (57.9%) of the isolates were from female. The prevalence of UTIs is high among patients with age between 16-30 years with the incident rate 20.9 %, age groups 1-15 years have the lower incidence rate 5.5%. UTIs were more prevalent among the rural area population, there were 50% than the population of the city center, there were 8.2% and the rate of infections were more prevalent among illiterate (no read and write) patients with incidence rate 44.5% , compared to literate (read and write) patients 13.6%. Also, our results showed that the sensitivity test against bacterial pathogens as All bacterial isolates was more sensitive to Ciprofloxacin, followed by Amikacin, Gentamycin , Cefotaxime (except Staphylococcus aureus Cefotaxime (42.9%) and Ceftriaxone (except Staphylococcus aureus) (28.6%). Staphylococcus aureus isolated also were sensitive to Erythromycin, Rifampicin, and Cephalothin. Finally, all isolates showed very high rate of resistance against Amoxicillin, ampicillin and penicillin G.

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References

Otajevwo, F.D. (2013). Urinary Tract Infection Among Symptomatic Outpatients Visiting a Tertiary Hospital Based in Midwestern Nigeria. Global Journal of Health Science, 5, 187-199.

Heising, P. (2010) Urinary tract infections and antibiotic resistance. Urologe.A, 49: 612-7.

Hooton, T.M., Roberts, P.L., Cox, M.E., Stapleton, A.E. (2013). Voided Midstream Urine Culture and Acute Cystitis in Premenopausal Women. New England Journal of Medicine, 369, 1883-1891.

Barnett, B. J. & Stephens, D. S. (1997). Urinary tract infection: an overview. Am J Med Sci, 314,245-249.

Ahmad, W., Jamshed, F. , Ahmad, W. (2015). Frequency of Escherichia coli in patients with community-acquired urinary tract infection and their resistance pattern against some commonly used antibacterials. J. Ayub. Med. Coll. Abbottabad., 27(2): 333-337.

Yusuf, M.A, Begum, A., Ahsan, C.R (2015). Antibiotic sensitivity pattern of gram-negative uropathogenic bacilli at a private hospital in Dhaka city. Al Ameen J Med Sci., 1: 189-194.

Burleigh, A.E., Benck, S.M., McAChran, S.E., Reed, J.D., Krueger, C.G. , Hopkins, W.J. (2013). Consumption of Sweetened, Dried Cranberries May Reduce Urinary Tract Infection Incidence in Susceptible Women—A Modified Observational Study. Nutrition Journal, 12, 139-145.

Eghafona, N.O., Evbagharu, P.A. ,Aluyi, H.A.S. (1998).Paediatric Urinary Tract Infection in Benin City, Nigeria. Journal of Medical Laboratory Sciences, 7, 59-61.

IGUA (international urogynecological association). (2011). Urinary Tract Infection.

Smith PJ, Morris AJ and Reller LB. (2003). Predicting urine culture results by dipstick testing and phase contrast microscopy. Pathology, 35: 161-165.

Smith, P. J, Morris, A. J ,Reller, L.B. (2003). Predicting urine culture results by dipstick testing and phase contrast microscopy. Pathology, 35: 161-165.

Cowan, S. T. ( 1975 ) . Manual for the Identification of Medical Bacteria . 2nd ed. Cambridge University Press. Cambridge, London. pp238.

Aypak, C., Altunsoy, A.,Duzgun, N.(2009). Empiric antibiotic therapy in acute uncomplicated urinary tract infections and fluoroquinolone resistance: a prospective observational study. Annals of clinical microbiology and antimicrobials,8: 27.

Gupta, K., Hooton,T.M, Stamm, W.E ( 2001). Increasing antimicrobial resistance and the management of uncomplicated community-acquired urinary tract infections. Annals of internal medicine,135(1): 41-50.

MacFaddin, J. F. (2000) Biochemical tests for identification of medical bacteria. 3rd ed. Philadelphia: Lippincott Williams and Wilkins.

Forbes BA. Sahm DF, Weissfeld AS. (2007).Bailey and Scott's Diagnostic microbiology, 12th edition, Mosby Elsevier; 842-55.

NCCLs-National Committee for Clinical Laboratory Standerds.(2007). PerformansStanderd for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement. M100-S17,Wayne,PA,USA.

Wayne, P.A. (2007). Performance Standards for Anti-microbial Susceptibility Testing. Clinical and Laboratory Standards Institute. 17th informational supplement. M100–S17.

Okafor, H.V., Okoro, B.A. and Ibe, B.C. (1993) Prevalence of Symptomatic Bacteriuria among Nursery School Children. Nigerian Journal of Paediatrics, 20, 84-88.

Thwaini , Q.N.A.(2002).Biology of cell wall defective microbes from persistentPyria&Persistent Hematuria patients .Ph . D.Thesis .Babylon University.

Nerurkar, A., Solanky, P., Naik, S. (2012). Bacterial pathogens in urinary tract infection and antibiotic susceptibility pattern. J Pharm Biomed Sci. 21: 1–3.

Zhanel, G.G (2005). Antibiotic resistance in outpatient urinary isolates: final results from the North American urinary tract infection collaborative alliance (NAUTICA). Int J Antimicrob Agents. 26: 380-8.

Andrade, S.S., Sader, H.S., Jones, R.N., Pereira, A.S., Pignatari, A.C., Gales, A.C. (2006). Increased resistance to first-line agents among bacterial pathogens isolated from urinary tract infections in Latin America: time for local guidelines? Mem Inst Oswaldo Cruz.; 101:741-8.

Russell, L.C., Freddy, M.C., Michael, B.S., Ralph, A.L. (2007).The predictability of urinary pathogens based on the urinalysis nitrite test in hospitalized patients. Hospital Pharm J. 42(1): 52-6.

Ronald, A. (2002)The etiology of urinary tract infection: Traditional and emerging pathogens. Am J Med. 113: Suppl 1A: 14S-9S.

Manjula, N.G., Girish, C.M., Shripad, A.P., Subhashchandra, M.G. and Channappa, T.S. (2013) Incidence of Urinary Tract Infections and Its Aetiological Agents among Pregnant Women in Karnataka Region. Advances in Microbiology,3, 473-478.

Getachew, T. (2010). Bacterial pathogens implicated in causing urinary tract infection (uti) and their antimicrobial susceptibility pattern in Ethiopia. RevistaENIC Ciencias Biol. 41:1–6.

Shahina, Z., JahedulIslam, M., Abedind, J., Ishaque, A.,Chowdhury, H, Arifuzzaman, D.(2011). A Study of Antibacterial Susceptibility and Resistance Pattern of E. coli Causing Urinary Tract Infection in Chittagong, Bangladesh.Asian J. Biol. Sci. 4: 548-555.

Sheffield, J.S. , Cunningham, F.G. (2005). Urinary tract infection in women. Obstetrics &Gynecology 106, 1085–1092.

Astal, Z.Y., Sharif, F.A. (2002). Relationship between demographic characteristics and community-acquired urinary tract infection. EMHJ. 8(1): 164-71.

Kothari, A., Sagar, V. (2008). Antibiotic resistance in pathogens causing community-acquiredurinary tract infections in India: a multicenter study. J Infect Developing Counties.2(5): 354-8.

Nerurkar, A., Solanky, P., Naik, S. (2012). Bacterial pathogens in urinary tract infection and antibiotic susceptibility pattern. J Pharm Biomed Sci. 21: 1–3.

Ashkenazi, S., Even, T.S., Samra, Z., Dinari, G.( 1991).Uropathogens of various populations and their antibiotic susceptibility. Paediatr Infect Dis J. 10:742-46.

Bannerman, T.L. (2003). Staphylococcus, Micrococcus, and other catalase positive cocci that grow aerobically. In: Murray, P.R.; Baron, E.J.; Jorgensen, J.H.; Pfaller, M.A.; and Yolken, R.H. (eds.). Manual of medical microbiology. American Society for Microbiology, Washington, DC, USA, pp. 384-404

Spauldinga, A. R.; Linb, Y.; Merrimana, J. A.; Brosnahana, A.J.; Petersonb, M.L.and Schlieverta, P.M. ( 2012). Immunity to Staphylococcus aureus secreted proteins protects rabbits from serious illnesses. Vaccine. 30:5099–5109.

Rahimi, E. and Alian, F. ( 2013). Presence of enterotoxigenicStaphylococcus aureusin cow, camel, sheep, goat, and buffalo bulk tank milk. VeterinarskiArhiv. 83 (1): 23-30.

Prakasam, K.C.; Dileesh, K. G., Vijayan, M.A.(2012).Cross-sectional study on the distribution of urinary tract infection and their antibiotic utilization pattern in Kerala.Int. J. Pharm.Tech Res.. 4(3): 1309-1316.

Strom BL et al. (1987). Sexual activity, contraceptive use, and other risk factors for symptomatic and asymptomatic bacteriuria. A case-control study. Ann Intern Med. 107: 816-23.

Shapiro, T., D., Dalton, M.D. (2005). The prevalence of Urinary tract infection and sexually transmitted disease in women with symptoms of a simple Urinary tract infection stratified by low colony count criteria” Academic Emergency medicine , 12, (1): 38-99 .

Oladeinde, B.H., Omoregie, R., Olley, M., Anunibe, J.A. (2011), Urinary Tract Infection in a Rural Community of Nigeria. North American Journal of Medical Sciences, 3, 75-77.

Lechellier, M.W., Seidler, R.J., Evans, T.M (1980).Enumeration and characterization of stander Plate Count Bacteria in Chlorinated and Raw water Supplies:40: 922-930.

Zhang, X.L., Zhu,Y. Z. , Yang Y. H. (1996). Vaginal colonization of S.aureus, A study in 267 cases of factory women chin. Med. J., 17(1); 17-9.

World health organization (1999). Guideline for drinking water quality, 2nd. Ed. Vol. 2:940- 949 .

CDC (Centers for Disease Control and Prevention) (2001). Methicillin-resistant Staphylococcus aureus skin of soft tissue infection in a state prison –Mississippi,2000.Morb.Mortal.Wkly.Rep.,50: 919-922.

Anyamene, C.O., Stellamaris, N. and MuonekeUmerie, G.N.C. (2002). Bacterial Isolates Associated with Urinary Tract Infections in Awka and Environs. Journal of Applied Sciences, 5, 3092-3098.

Onanuga, A., Oyi, A.R., Olayinka, B.O., Onaolapo, J.A. (2005).Prevalence of community-associated multi-resistant Staphylococcus aureus among healthy women in Abuja, Nigeria. Afr. J. Biotech. 4:942-5.

Orrett, F.A, Davis, G.K. (2006).A comparison of antimicrobial susceptibility profile of urinary pathogens for the years, 1999 and 2003.West Indian Med J. 2006; 55(2): 95-9.

Bujdakova, H., Lausova, A., Jankovicova, S. (1998). Study Of β-lactam Resistance in Ceftazidime-Resistance Clinical Isolates Of Enterobacteriaceae. Intern.J.ofAntimicrob. Agents 10: 135-41

Cherian, B.P., Manjunath, M., Pinto Pereira, L.M., Prabhakar, P.(2003). Extended-spectrum beta-lactamase-producing Enterobacteriaceae in a tertiary care hospital in Trinidad and Tobago. West Indian Med.J. Mar.52(1): 31-3.

Rubin, R.H. (1999). Infection in the Renal transplant recipient. The American J. of Medicine . 70 : 420-425.

Hooper, D.C.(2000). Quinolones. P. 404-423. In: G.L.Mandell, J.E.Bennett, R.Dolin(ed). Mandell, Douglas, &Benett,s Principles and Practice Infections Disease. 5th ed . Churchill Livingstone, Philadelphia.

Leblebicioglu, H., Gunaydin, M., Esen, S., Tuncer, I. (2002). Surveillance of antimicrobial resistance in Gram-negative isolate from intensive care units in Turkey. J. Chemotherapy ; 14(2) : 6-140.

Ebrahimzadeh, M.A, Mahdavee, M.R, Vahedi, M. (2005). Antibiotic resistance in E. coli isolated from urine: a 2-year study on isolates from patients with urinary tract infections in Iran. Journal of Cell and Tissue Research. 5:445-448

Gupta, K., Hooton, T.M., Stamm, W.E (2001(.Increasing antimicrobial resistance and the management of uncomplicated community-acquired urinary tract infections. Ann InternMed. 135: 41-50.

Orrett, F.A., Davis, G.K. (2006).A comparison of antimicrobial susceptibility profile of urinary pathogens for the years, 1999 and2003.West Indian Med J. 55(2): 95-9.

Shigemura, K., Tanaka, K., Okada, H., Nakano, Y., Kinoshita, S., Gotoh, A., Arakawa, S., Fujisawa, M. (2005).Pathogen occurrence and antimicrobial susceptibility of urinary tract infection cases during a 20-year period (1983-2002) at a single institution in Japan. Jpn J infects Dis .58:303-8.

Manikandan, S., Ganesapandian, S., Singh, M., A.K. Kumaraguru, A. (2011). Antimicrobial Susceptibility Pattern of Urinary Tract Infection Causing Human Pathogenic Bacteria. Asian Journal of Medical Sciences 3(2): 56-60.

Nerurkar, A., Solanky, P., Naik, S. (2012). Bacterial pathogens in urinary tract infection and antibiotic susceptibility pattern. JPharm Biomed Sci. 21(12)