Physiological and histological effects of (zinc and iron) oxide nanoparticles on some fertility parameters in female mice


  • Shaima R. Ibraheem Biotechnology department, Collage of Science, Baghdad University.
  • Muntaha R. Ibrahim Biomedical department, Alkawarizmi Collage of engineering, Baghdad University.



Nanoparticles, fertility, hormones, mice


Nowadays nanoparticles have widespread application in various industries
because of their special and unique features, there are many studies in side
effects of nanomaterial. This study done by 40 white female mice with
every other day intraperitoneally injection of low and high doses of both of
ZnO kg of body weight) and FeO
nanoparticles (5 and 40 mg/kg). After a 15 days period, the mice were
sacrificed and blood samples were collected for hormone analysis, and
tissue samples for morphometric studies.
Statistical Analysis shows significant differences in LH, Estrogen,
Progesterone hormone levels between groups, while there are insignificant
differences in Follicle stimulating hormone (FSH) level between the
groups compared with its level in the control group.
The results also show that the highest level of LH reach 7.2 mIU/ml in the
groups treated with low dose of zinc oxide, the highest level of FSH reach
4.58 mIU/ml in the groups treated with low dose of zinc oxide, the highest
level of Estrogen hormone reach 69.5 ng/ml in the groups treated with low
of dose zinc oxide and the highest level of Progesterone reach 1.9 ng/ml in
the groups treated with high dose iron oxide. We conclude from the results
that the low doses of ZnO has benefits in increasing fertility through high
level of reproductive hormones, while the high levels of nanoparticles
reduce fertility and there is a relation between FeO nanoparticles and
progesterone levels which may need more future studies.
Morphometric study of the ovary show increase in Follicular stages
number range in the group treated with Low dose ZnO in compare with its
range in the control groups. The lower range was belong to the group
treated with the high dose of FeO. No significant differences has been
found in the diameter mean of the different follicular phases between the
group treated with low dose of ZnO NPs in compared with the control
group. High dose of ZnO NPs cause significant increase in the diameter
mean of Primordial follicles in compared with the control group. Low and
high dose FeO NPs treated groups show significant reduction in the
diameter mean of the different follicular phases in compared with the
control group.


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How to Cite

S. R. Ibraheem and M. R. Ibrahim, “Physiological and histological effects of (zinc and iron) oxide nanoparticles on some fertility parameters in female mice”, Al-Mustansiriyah Journal of Science, vol. 27, no. 5, pp. 1–10, Jul. 2017, doi: 10.23851/mjs.v27i5.160.

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