Some Immunological Aspects of Iraqi Influenza A Virus Infections

Authors

  • Raghad H. Al-Azzawi Department of Biology, College of Science, University of Baghdad

DOI:

https://doi.org/10.23851/mjs.v28i3.549

Keywords:

influenza A virus, CRP, IL-6, IL-1 alpha.

Abstract

In the last years, the world has been facing pandemic influenza which caused by influenza A virus. In this study detection of influenza A virus infection in Iraqi patients was done by r RT-PCR. Three hundred eighty (380) clinical respiratory secretions samples that were collected between December and April 2013, from different hospitals and they were sent to Central Public Health Laboratory (CPHL)/National Influenza center/ Baghdad /Iraq. Among these samples, only 35 samples (22 Males and 13 females all of them were adults) gave positive results (both TS (throat swabs) and NPS (nasopharyngeal swab)) for influenza A virus (9.2%). Interleukin-6 (IL-6) and IL-1 α are pleiotropic cytokines implicated in the pathogenesis of local inflammation during viral upper respiratory infections. Cytokines levels in both NPS and TS were determined. The result appeared that there was a significant difference between IL-6 and IL-1α, but there was no significant difference between IL-6 and IL-1 α in Ts and NSP. For the first time we try to detect C-reactive protein (CRP) in nasopharyngeal swab (NPS) and throat swabs (TS) and compared with serum, also to prove that this protein may be secreted in fluids other than serum. The result showed that 64.3% of both NPS and TS were positive for CRP. This may be due to that CRP in response to microbial infection, tissue injury, and immunomodulatory stimuli are synthesized and released by various cells.

References

World health organization Influenza (Seasonal) Fact sheet NO 211, March 2014.

World health organization "Key Facts about Influenza (Flu) and Flu Vaccine". September 9, 2014. Retrieved 26 November 2014.

Klenk, Hans-Dieter; Matrosovich, Mikhail;Stech,Jürgen.(2008). "Avian Influenza: Molecular Mechanisms of Pathogenesis and Host Range". In Mettenleiter, Thomas C.; Sobrino, Francisco. Animal Viruses: Molecular Biology. Caister Academic Press.ISBN 978-1-904455-22-6. DOI: https://doi.org/10.1159/isbn.978-3-8055-8502-6

Wenjie Z; John O; Vikram V; and Yizhi J T.( 2013).The Crystal Structure and RNA-Binding of an Orthomyxovirus Nucleoprotein. PLoS Pathog. 9(9): e1003624. DOI: https://doi.org/10.1371/journal.ppat.1003624

Stephanie A. Irving, Mary F. Vandermause, David K. Shay, and Edward A.( 2012). Belongia. Comparison of Nasal and Nasopharyngeal Swabs for Influenza Detection in Adults.Clinical Medicine and Research.10 (4): 215-218.

Estella,A.( 2011). Cytokine levels in bronchoalveolar lavage and serum in 3 patients with 2009 influenza A(H1N1)v severe pneumonia. Journal of Infection in Developing Countries. 5, ( 7): 540–543. DOI: https://doi.org/10.3855/jidc.1618

Antonio ,C, Silvia P., Giovanni B., Pietro F., Valerio R., Domenico C., and Riccardo R.( 2013). IL-1β and IL-6 Upregulation in Children with H1N1 Influenza Virus Infection. Mediators of Inflammation. DOI: https://doi.org/10.1155/2013/495848

R. Le Goffic, M. I. Arshad, M. Rauch.( 2011 ). “Infection with influenza virus induces IL-33 in murine lungs,” The American Journal of Respiratory Cell and Molecular Biology. 45( 6):1125–1132. DOI: https://doi.org/10.1165/rcmb.2010-0516OC

A. G. Besnard, D. Togbe, N. Guillou, F. Erard, V. Quesniaux, and B. Ryffel. (2011 ) “IL-33-activated dendritic cells are critical for allergic airway inflammation,” European Journal of Immunology. 41 (6): 1675–1686. DOI: https://doi.org/10.1002/eji.201041033

Russell RJ, Kerry PS, Stevens DJ, Steinhauer DA, Martin SR, Gamblin SJ, Skehel JJ. (2008)."Structure of influenza hemagglutinin in complex with an inhibitor of membrane fusion". Proc. Natl. Acad. Sci. U.S.A. 105 (46): 17736. DOI: https://doi.org/10.1073/pnas.0807142105

Nelson DL, Cox MM. Lehninger's Principles of Biochemistry (4th ed.). New York: WH Freeman. (2005).

Bustin, S. A and Mueller, R.(2005).Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis. Clinical Science.) 109: 365–379. DOI: https://doi.org/10.1042/CS20050086

WHO. (2013). H1N1 influenza cases reported in Iraq, 5 February.

Afrasiabian ,S . ; Mohsenpour, B.; Bagheri, K.H.; Barari, M.; Ghaderi, E.; Hashemi, R.; and Garibi , F.( 2014). Epidemiological survey on pandemic influenza A (H1N1) virus infection in Kurdistan province, Islamic Republic of Iran, 2009. EMHJ.20 (3). DOI: https://doi.org/10.26719/2014.20.3.169

de la Tabla VO, Masiá M, Antequera P, Martin C, Gazquez G, Buñuel F, Gutiérrez F.( 2010). Comparison of combined nose-throat swabs with nasopharyngeal aspirates for detection of pandemic influenza A/H1N1 2009 virus by real-time reverse transcriptase PCR. J Clin Microbiol. 48:3492–3495. DOI: https://doi.org/10.1128/JCM.01105-10

Sung RY, Chan PK, Choi KC, Yeung AC, Li AM, Tang JW, Ip M, Tsen T, Nelson EA. (2008). Comparative study of nasopharyngeal aspirate and nasal swab specimens for diagnosis of acute viral respiratory infection. J Clin Microbiol . 46:3073–3076. DOI: https://doi.org/10.1128/JCM.01209-08

Stephanie A. I., Mary F. V.‡, David K. S., and Edward A. B. (2012). Comparison of Nasal and Nasopharyngeal Swabs for Influenza Detection in Adults. Clinical Medicine & Research. 10 ( 4 ): 215-218. DOI: https://doi.org/10.3121/cmr.2012.1084

Lambert SB, Whiley DM, O'Neill NT, Andrews EC, Canavan FM, Blethly C, Siebert DJ, Sloots TP, Nissen MD. (2008). Comparing nose-throat swabs and nasopharyngeal aspirates collected from children with symptoms for respiratory virus identification using real-time polymerase chain reaction. Pediatrics. 122:e615–e620. DOI: https://doi.org/10.1542/peds.2008-0691

Calabro P, Willerson JT, Yeh ET. (2003). Inflammatory cytokines stimulated C-reactive protein production by human coronary artery smooth muscle cells. Circulation. 108:1930–2. DOI: https://doi.org/10.1161/01.CIR.0000096055.62724.C5

Venugopal SK, Devaraj S, Jialal I. (2005). Macrophage conditioned medium induces the expression of C-reactive protein in human aortic endothelial cells: potential for paracrine/autocrine effects. Am J Pathol. 166:1265–71. DOI: https://doi.org/10.1016/S0002-9440(10)62345-0

Maier W, Altwegg LA, Corti R. (2005). Inflammatory markers at the site of ruptured plaque in acute myocardial infarction: locally increased interleukin-6 and serum amyloid a but decreased C-reactive protein. Circulation. 111:1355–61. DOI: https://doi.org/10.1161/01.CIR.0000158479.58589.0A

Haider, D G ; Leuchten, N ; Schaller, G; Gouya , G ; Kolodjaschna , Kolodjaschna; Schmetterer, L ; Kapiotis , S; Wolzt, M. (2006). C-reactive protein is expressed and secreted by peripheral blood mononuclear cells.Clin Exp Immunol. 146(3): 533–539. DOI: https://doi.org/10.1111/j.1365-2249.2006.03224.x

Jabs WJ, Logering BA, Gerke P. (2003). The kidney as a second site of human C-reactive protein formationin vivo. Eur J Immunol. 33:152–61. DOI: https://doi.org/10.1002/immu.200390018

Moshage HJ, Roelofs HM, van Pelt JF. (1988). The effect of interleukin-1, interleukin-6 and its interrelationship on the synthesis of serum amyloid A and C-reactive protein in primary cultures of adult human hepatocytes. Biochem Biophys Res Commun. 155:112–17. DOI: https://doi.org/10.1016/S0006-291X(88)81056-8

Pue CA, Mortensen RF, Marsh CB, Pope HA, Wewers MD. (1996 ). Acute phase levels of C-reactive protein enhance IL-1β and IL-1ra production by human blood monocytes but inhibit IL-1β and IL-1ra production by alveolar macrophages. J Immunol. 156:1594–600.

Bedaiwy MA, Falcone T. (2003).Peritoneal fluid environment in endometriosis. Clinicopathological implications. Minerva Ginecol. 55(4):333–45.

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Published

28-07-2018

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Section

Biological Science

How to Cite

[1]
R. H. Al-Azzawi, “Some Immunological Aspects of Iraqi Influenza A Virus Infections”, Al-Mustansiriyah Journal of Science, vol. 28, no. 3, pp. 102–108, Jul. 2018, doi: 10.23851/mjs.v28i3.549.

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