Hematological and biochemical changes in rabbits exposed to castor oil (Ricinus communis) under experimental conditions


  • Sahar M. Ahmed Al-Rafidain University College




In the last few decades there has been an exponential growth in the field of herbal medicine. One such medicinal plant is Ricinus communis (Euphorbiaceae), which is commonly known as castor. All parts of the plant are important phloem, bark, leaves, flowers, seed and oil. The study was conducted on 15 mature rabbitsof either sex of 1-2 kg body weight and 1-2 years old. The animals were divided into three groups of 5 animals each. Animals of group I were exposed orally to ricin extract at a dose rate of 0.5 mg /kg b.wt. daily for 14 days, while those of group II were exposed orally to aqueous leaves extract 0.5mg /kg b.wt daily for 14 day, mean while those of group III were left as a control group not exposed. The dependent parameters in the study were hemoglobin (Hb) concentration, total erythrocytes count, packed cells volume (PCV%), erythrocytes indices mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), Total and differential leucocytes count (TLC and DLC), in addition to some biochemical tests of blood serum which obtained at day 14th post exposure. The results of the study were revealed that the ricin extract and leaf extract exhibited an effects on hematological pictures as the erythrocytes counts, erythrocytes indices, Hb concentration and PCV% decreased and the obvious effects were in the 14th day. Ricin extract was less effects on many dependent parameters in comparison with aqueous leaf extract. Total leucocytes count, neutrophils % was increased in both ricin and leaf extract, and the increasing were higher in the 7th day in Ricin extract group. The lymphocytes% was decreased. While monocytes, eosinophils, and basophils % did not show any significant changes in all groups. Neutrophil /lymphocyte (N/l), and monocyte /lymphocyte (M/l) increased in both exposed groups. Cholesterol (Chol), Triglyceride (TG)were increased, while total protein (TP)was decreased, Albumin (Alb), Cortisol (Cort), Total serum bilirubin (TSB) and Alanine aminotransferase (ALT)did not changed. Aspartate aminotransferase (AST) value was increased, while Adenosine phosphatase (AP) value was deceased. In conclusion castor oil due to its content of ricin exhibit an effects on hematological and biochemical parameters that depended in the study, and the leaves extract was higher than seed extract in its effects.


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I. Ross, First ed. Medicinal Plants of the world: Chemical Constituents Traditional and Modern Medicinal Uses, Totowa, New Jersey: Humana Press, 2001. E. Williamson, Major Herbs of Ayurveda, China: Churchill Livingstone, 2002.

Byamukama, R., Jordheim, M., Kiremire, B., Anderson, O.M., "New anthocyamins from the stem bark of castor, Ricinus communis, " Natural Product Communications, vol. 3, pp. 1497-1500, 2008.

Kang, S.S., Cordell, G.A., Soejarto, D.D., Fong, H.H.S., "Alkaloids and flavonoids from Ricinus communis, " Journal of Natural Products, vol. 48, pp. 155-156,1985. CrossRef

Tan, Q.G., Cai, X.H., Du, Z.Z., Luo, X.D., "Three terpenoids and tocopherol-related compound from Ricinus communis, " Helvetica Chemica Acta, vol. 92, pp. 2762 -2768, 2009. CrossRef

J. Jerkins, "Allergenic and toxic components of castor oil bean meal: Review of literatures and studies of inactivation of these components, " J. Sci. Food Agric., vol. 14, pp. 773-780, 1963. CrossRef

Al-Tahan, F.J. and Al-Shaha, O. M. S., "A primary study on castor beans cultivated in Iraq and its content of the toxic substance ricin, " in The proceeding of the 2nd Technical Education Conference, Baghdad, Iraq, 1990.


E. Coles, Veterinary Clinical Pathology, Philadelphia, USA: 4th ed. W.B.Saunders Company, 1986.

SPSS, Writer, SPSS Version 15 for window. [Performance]. SPSS Inc., 2001.

Abrami, L.R.N., and Gisou Van, G.F., "Anthrax toxin: The long and winding road that leads to the kill, " Trends Microb, vol.13, pp. 72-78, 2005. CrossRef


Celikel, N., and Kavas, G., "Antimicrobial properties of some essential oils against some pathogenic microorganism, " Czech J. Food Sci, vol. 26, pp. 174-181, 2008.

Momoh, A.O., Oladunmoye, M. K. and Adebolu, T. T., "Haematological and histopathological effects of oil from castor seeds (Ricinus communis Linn) on albino rats., " Journal of Pharmacognosy and Pytotherapy, vol. 4, no. 4, pp. 40-43, 2012. CrossRef

Kumar, O., Sugendran, K., and Vijayaraghavan, R., "Oxidative stress associated hepatic and renal toxicity induced by ricin in mice, " Toxicon, vol. 41, pp. 333-338, 2003. CrossRef

Aslani, M.R., Malaeki, M., Mohri, M., Sharifi, K., Najiar-Nezhad, V. and Afshari, ER., "Castor bean (Ricinus communis) toxicosis in a sheep flock, " Toxicon, vol. 49, no. 3, pp. 400-406, 2007. CrossRef


Sylvia W., Kernt K., Diana P., Mare-Andre A., Martin S., Martin, B.D., and Brigitte G.D., "Ricinus communis Intoxications in Human and Veterinary Medicine a Summary of Real cases, " Toxins, vol. 3, pp. 1332-1372, 2011. CrossRef PubMed




How to Cite

S. M. Ahmed, “Hematological and biochemical changes in rabbits exposed to castor oil (Ricinus communis) under experimental conditions”, Al-Mustansiriyah Journal of Science, vol. 28, no. 1, pp. 19–24, Nov. 2017.



Biological Science