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Influence of Helium-Neon Laser on Some Virulence Factors of Staphylococcus Aureus and Escherichia Coli


 
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1. Title Title of document Influence of Helium-Neon Laser on Some Virulence Factors of Staphylococcus Aureus and Escherichia Coli
 
2. Creator Author's name, affiliation, country Mohammed F. Abo-Ksour; Biology Department, College of Science, Mustansiriyah University, IRAQ.; Iraq
 
2. Creator Author's name, affiliation, country Sawsan S. Al-Jubori; Biology Department, College of Science, Mustansiriyah University; Iraq
 
2. Creator Author's name, affiliation, country Hussein A. Jawad; Biology Department, College of Science, Mustansiriyah University; Iraq
 
3. Subject Discipline(s) biology
 
3. Subject Keyword(s) He-Ne laser, virulence factors, DNA plasmid, β-lactamase, hemolysin, S. aureus, E. coli.
 
4. Description Abstract This work aims to investigate the effect of Helium-Neon laser on locally isolated Staphylococcus aureus & Escherichia coli bacteria; their resistance pattern, virulence factors, and their plasmid content. Bacteria were collected from patient suffering from urinary tract infections, septicemia, wound, and burn infections, then isolates were identified according to their characteristics, features; morphological, biochemical tests, and API systems. Out of Eighty-seven samples, twenty-seven isolates of S. aureus and thirteen isolates of E. coli were isolated. The results showed that after laser treatment, the diameter of inhibition zone increased for almost of the used antibiotics beside some isolates became sensitive especially after 2 min of laser exposure time. The hemolysin production was disappeared in (%40) of E. coli and (%20) of S. aureus after ten min of irradiation, while it decreased to half in another S. aureus isolate. All S. aureus and E. coli isolates loosed their ability to produce β-lactamase enzymes; some of these isolates loosed it after 5 minutes and the others after 10 minutes of irradiation. The results showed that laser irradiation hasn't any effect at any exposure time on adhesion factors of both S. aureus and E. coli isolates. Plasmid profile of Irradiated E. coli illustrated disappearing of DNA plasmid bands as well as RNA after ten min of irradiation.
 
5. Publisher Organizing agency, location Mustansiriyah University
 
6. Contributor Sponsor(s)
 
7. Date (YYYY-MM-DD) 2019-03-10
 
8. Type Status & genre Peer-reviewed Article
 
8. Type Type
 
9. Format File format PDF
 
10. Identifier Uniform Resource Identifier http://mjs.uomustansiriyah.edu.iq/ojs1/index.php/MJS/article/view/619
 
10. Identifier Digital Object Identifier (DOI) http://dx.doi.org/10.23851/mjs.v29i3.619
 
11. Source Title; vol., no. (year) Al-Mustansiriyah Journal of Science; Vol 29, No 3 (2018): ICSSSA 2018 Conference Issue
 
12. Language English=en en
 
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.)
 
15. Rights Copyright and permissions Copyright (c) 2019 Al-Mustansiriyah Journal of Science
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