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Detection of blaCTX-M gene among Pseudomonas aeruginosa isolated from water samples in Baghdad


 
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1. Title Title of document Detection of blaCTX-M gene among Pseudomonas aeruginosa isolated from water samples in Baghdad
 
2. Creator Author's name, affiliation, country Saba R. Khdair; Department of Biology, College of Science, Mustansiriyah University; Iraq
 
3. Subject Discipline(s) Biology
 
3. Subject Keyword(s) Pseudomonas aeruginosa, Sewage, blaCTX-M gene.
 
4. Description Abstract A total of 50 environmental Pseudomonas aeruginosa isolates were collected from sewage and tap water in Baghdad, Iraq. The MICs of Cefotaxime and Ceftazidime were determined by using agar dilution method, The MIC ranged from 2 to 256 µg/ml.The results of antibiotic sensitivity test showed that among sewage P. aeruginosa isolates, resistance was observed most often to Ticarcillin (92%), Penicillin G (84%), Ceftazidime (12%), (8%) for each of Cefotaxime and Ticarcillin. On the other hand, all tap water isolates were sensitive to Ofloxacin and Levofloxacin, Except (5%) of isolates were resistant to Cefotaxime (25%) to Ceftazidime and (95%) to Ticarcillin. All isolates were tested for Extended-Spectrum β-Lactamase (ESBL) production. Ten isolates (20%) were found to be ESBL producers. All environmental P. aeruginosa isolates were screened for the presence of the blaCTX-M genes by application PCR, Only (30%) of them were positive for this test.
 
5. Publisher Organizing agency, location Mustansiriyah University
 
6. Contributor Sponsor(s) Mustansiriyah University
 
7. Date (YYYY-MM-DD) 2017-11-19
 
8. Type Status & genre Peer-reviewed Article
 
8. Type Type
 
9. Format File format PDF
 
10. Identifier Uniform Resource Identifier http://mjs.uomustansiriyah.edu.iq/ojs1/index.php/MJS/article/view/309
 
10. Identifier Digital Object Identifier (DOI) http://dx.doi.org/10.23851/mjs.v28i1.309
 
11. Source Title; vol., no. (year) Al-Mustansiriyah Journal of Science; Vol 28, No 1 (2017)
 
12. Language English=en en
 
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.)
 
15. Rights Copyright and permissions Copyright (c) 2017 Al-Mustansiriyah Journal of Science
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