Effect of Bacterial Isolates From Soil Samples on Bisphenol A

Dr.Neihaya Heikmat Zaki

Abstract


Twenty five samples were collected from the soil around the Tigris River from different locations in Iraqi cities, and 45 bacterial isolates were obtained. Three of these isolates were further tested for their degrading capacity of Bisphenol A (BPA) in Basal Mineral Medium, included: Pseudomonas orizohibtanis, Escherishia coli and Proteus penneri. The optimal temperature for the removal of BPA was determined at 20˚C, 37˚ and 45˚C for 1, 5, and 15 days, and the degradation increased up to a temperature of 37°C. Growth test was performed on isolated bacteria with BisPhenol A as the sole carbon source, and with increasing incubation time, the culture grew almost linearly to 24 hours. BPA decreased after 1days after incubating with tested bacterial isolates, and almost broken after 5 days, while it disappeared after 15 days at 37C, and Pseudomonas orizohibtanis exhibited the best degradation of BPA. The absorbance peaks in the UV region appeared at 222 and 276 nm and attributed to the benzene ring and triazine ring respectively. The end products of BPA degradation were analyzed by GCMS after 15 days of incubation. The chromatogram for Pseudomanas orizohibtanis showed three peaks at retention times of 70, 210 and 280 min, and referred to hexasiloxane, heptasiloxane, and Octasiloxane respectively. The present study was aimed to isolate bacteria from the soil of the Tigris River, and determined the ability to degrade Bisphenol-A, and characterized the environmental conditions of bacterial growth, and then analysis the products of the degradation by GC-MS.

Keywords


Bacterial degrading isolates, Bisphenol-A, degradation, Optimization, GC-MS.

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References


J. Kang, Y. Katayama, and F. Kondo, Biodegradation or metabolism of bisphenol A: From microorganisms to mammals. Toxicology, 217, 2006, pp. 81-90. Crossref | PubMed

G.Yang, Y. He, Z. Cai, X. Zhao, and L. Wang, Isolation and characterization of Pseudomonas putida WLY for reactive brilliant red x-3b decolorization. African Journal of Biotechnology, Vol. 10(51): 2011, pp. 10456-10464. Crossref

J. Kito, and K. Kondo, Factors influencing the migration of bipshenol A from cans, J. Food Protect. 66, 2003, pp. 1444-1447. Crossref | PubMed

J. Kang, N. Ri, and F. Kondo, Streptomyces sp. strain isolated from river water has high bisphenol A degradability, Letters in Applied Microbiology, 39, 2004, pp.178-180. Crossref | PubMed

A. Soh, A. Lim, and C. Loke, Isolation and characterization of a phenol-degrading bacterium from an industrial activated sludge, Appl. Microbiol. Biotechnol. 2006, 71, 728-735. Crossref | PubMed

V. Arutchelvan, V. Kanakasabai, R. Elangovan, S. Nagarajan, and V. Muralikrishnan, Kinetics of high strength phenol degradation using Bacillus brevis. J. Hazard. Mater. 129, 2006, pp. 216-222. Crossref | PubMed

R. Sun, M. Wang, and S. Deng, Degradation of phenolic compounds with hydrogen peroxide catalyzed by enzyme from Serratia marcescens AB 90027. Water Res. 40, 2006, pp. 3091-3098. Crossref | PubMed

A. Mahesh, M. Nagenahalli, and D. Yun, Phenol degradation by immobilized cells of Arthrobacter citreus. Biodegradation, 2006, 17, pp. 47-55. Crossref | PubMed

Z. Liu, Y. Xie, D. Li, Y. Peng, Z. Li, and S. Liu, Biodegradation of Phenol by Bacteria Strain Acinetobacter Calcoaceticus PA Isolated from Phenolic Wastewater. Int. J. Environ. Res. Public Health, 13, 2016, pp. 300. Crossref | PubMed

K. Chen, J. Wu, D. Liou, S. Hwang, Decolorization of the textile dyes by newly isolated bacterial strains, J. Biotechnol. 101: 2003, pp. 57-68. Crossref | PubMed

J. Maura, A. Meade, L. Rebecca, A. Waddell, and M. Terrence, Soil bacteria Pseudomonas putida and Alcaligenes xylosoxidans subsp. denitricans inactivate triclosan in liquid and solid substrates. FEMS Microbiology Letters, 204, 2001, pp. 45:48. Crossref | PubMed

X. Dong, and M. Cai, Manual of Bacteria Identify, Science Press, Beijing. Forgacs E, Cserháti T, Oros G. Removal of synthetic dyes from wastewaters: a review. Environ. Int. 30: 2004, pp. 953-971. Crossref | PubMed

L. Wang, L. Yu, C. Chen, E. Li, and Z. Cai, Study of decolorization of active brilliant red X-3B by a pseudomonas strain. J. Jiangsu polytech. Univ. 21(2): 2009, pp. 15-18.

E. Nishio, Y. Ichiki, H. Tamura, S. Morita, K. Watanabe, and H. Yoshikawa, Isolation of Bacterial Strains that Produce the Endocrine Disruptor, Octylphenol Diethoxylates, in Paddy Fields, Biosci. Biotechnol. Biochem., 66 (9), 2002, pp. 1792-1798. Crossref | PubMed

X. Xu, H. Lia, and J. Gu, Biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate ester by Pseudomonas flouorescens B-1. International Biodeterioration & Biodegradation. 2004, Pp.1-7. Crossref

A. Telkea, D. Kalyania, C. Umesh, U. Ganesh, and K. Sanjay, Purification and characterization of an extracellular laccase from a Pseudomonas sp. LBC1 and its application for the removal of bisphenol A. Journal of Molecular Catalysis B: Enzymatic, 61, 2009, pp. 252-260. Crossref

M. Sasaki, J. Maki, K. Oshiman, Y. Matsumura, T. Tsuchido, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1, Biodegradation, 16: 2005a, pp. 449-459. Crossref | PubMed

L. Ahrens, Polyfluoroalkyl compounds in the aquatic environment: A review of their occurrence and fate. Journal of Environmental Monitoring, 13(1): 2011, pp.20-31. Crossref | PubMed

J. Kang, and F. Kondo, Effects of bacterial counts and temperature on the biodegradation of bisphenol A in river water. Chemosphere, 49: 2002, pp.493-498. Crossref

S. Maulin, Microbiological Removal of Phenol by an Application of Pseudomonas spp. ETL-: An Innovative Biotechnological Approach Providing Answers to the Problems of FETP. Journal of Applied & Environmental Microbiology, Vol. 2, No. 1, 2014, pp. 6-1.

J. Moreta, Diversity of ascomycete laccase sequences and contributions of bacteria and ascomycetous fungi to lignocelluloses Degradation in a southeastern U.S. salt marsh. Thesis of master of science, university of Georgia, 1997.

I. Castro, J. Becker, K. Dohnt, V. Dos Santos, and C. Wittmann, Industrial biotechnology of Pseudomonas putida and related species. App. Microbial Biotechnol., 9: 2012, pp. 2279 -2290. Crossref | PubMed

M. Lakshmi, V. Sridevi, M. Rao, and A. Swamy. Substrate inhibition kinetics of phenol degradation by pseudomonas aeruginosa (ncim 2074). International Journal of Environmental Pollution Control & Management. Vol. 3, No. 1, 2011, pp. 103-113.

a. Spivack, Novel pathway for bacterial metabolism of bisphenol A. Rearrangements and stilbene cleavage in bisphenol A metabolism. Journal of Biological Chemistry, 269(10): 1994, 7323-9.

M. Sasaki, A. Akahira, K. Oshiman, T. Tsuchido, and Y. Matsumura, Purification of cytochrome P450 and ferredoxin, involved in bisphenol A degradation, from Sphingomonas sp. strain AO1. Appl Environ Microbiol, 71: 2005b, pp. 8024-8030. Crossref | PubMed

T. Hirano, Y. Honda, T. Watanabe, and M. Kuwahara, Degradation of bisphenol A by the lignin-degrading enzyme, Biodegradation, 22: 2011, pp. 389-396.

H. Uchida, F. Tetsuya, M. Hideo, U. Takayuki, Polymerization of Bisphenol A by Purified Laccase from Trametes villosa. Biochemical and Biophysical Research Communications, 287(2): 2001, pp. 355-8. Crossref | PubMed

F. Dunnivant, and J. Ginsbach, A basic introduction: gas chromatography, liquid chromatography, capillary electrophoresis-mass spectrometry. 2011, USA: Whitman.




DOI: http://dx.doi.org/10.23851/mjs.v31i1.686

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