Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant

Ali A. Taha, Nahida J. Hameed, Farah Hamed Ali

Abstract


Polycyclic aromatic hydrocarbons (PAHs) are recognized as a toxic, mutagenic and/or carcinogenic compounds, and their pollution of soil and aquifer is of increasing environmentally risk. Laccases (E.C. 1.10.3.2) are phenoloxidases catalyze the oxidation of PAHs in the presence of a mediator compound and hyacinth plant. In this study laccase from Trametes versicolor was immobilized into chitosan, and the potential to oxidize anthracene in the presence of 1-hydroxybenzotriazole (HBT) was examined. Results indicated that the immobilization enhanced the stability of laccase against temperature, pH, inhibitors and loading time compared with the other cases. The immobilized laccase-mediator system was as efficient as the free enzyme for oxidizing the tested PAHs. After 24h. of incubation, immobilized laccase–HBT showed a system oxidization more than immobilized laccase without (HBT) of PAHs; Chitosan with hyacinth plant and (HBT) resulted better conversion than chitosan with or without HBT. These results indicate a new chance for applying the immobilized laccase in bioremediation.

Keywords


Polycyclic aromatic hydrocarbons PAH; Immobilized laccase; Trametes versicolor; Bioremediation; HBT; Conversation ; Hyacinth plant.

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DOI: http://dx.doi.org/10.23851/mjs.v31i3.670

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