Antimicrobial Activity of Freshwater Cyanobacterium Westiellopsis prolifica

Abstract

The acetone and hexane of Westiellopsis prolifica extracts were examine efficiency against patho-genic bacterial and fungal isolates by using two methods: agar well diffusion and turbidiometric (tube method) against three Gram positive bacteria"Staphylococcus aureus, Bacillus subtilis, and Streptococcus sp." and three Gram- negative bacteria" Shigella sp., Proteus sp. and Pseudomonas aeruginosa " in additions to two isolates of fungi "Aspergillus niger and Candida albicans". The re-sults showed that crude acetone extract for W. prolifica better than the haxane extract and more efficient on negative gram bacteria than positive gram bacteria. The results of the agar well diffusion method evaluated that W. Prolifica acetone extract has the highest antibacterial activities against Streptococcus sp., S. aureus and A.niger with an inhibition zone of (20) mm, and the inhibition diam-eter to other bacteria and fungi were between(15-10) mm.While tube method showed that the ace-tone extract exhibited the highest inhibition against A.niger and less inhibiting to C. albicans. Purifica-tion of the acetone extracts was made by silica gel column chromatography, and among the five groups extracts, Group 2 (Benzene 50ml) was selected and analyzed by GC-MS. The presence of main components identified in the extract as alcohols, acids, monoterpene eucalyptol, hydrocarbons (unidecane) aromaticslike, Para- Xylene and 1,2,3 trimethyl benzene, Phytol, n-Hexadecanoic acid, etc. These purified active compounds take part into broad horizons in the fields of biotechnology and pharmacy.