Antimicrobial Activity of Freshwater Cyanobacterium Westiellopsis prolifica

Ghaidaa Al-Rrubaie, Neihaya Heikmat Zaki, Shurooq Latif

Abstract


The acetone and hexane of Westiellopsis prolifica extracts were examine efficiency against patho-genic bacterial and fungal isolates by using two methods: agar well diffusion and turbidiometric (tube method) against three Gram positive bacteria"Staphylococcus aureus, Bacillus subtilis, and Streptococcus sp." and three Gram- negative bacteria" Shigella sp., Proteus sp. and Pseudomonas aeruginosa " in additions to two isolates of fungi "Aspergillus niger and Candida albicans". The re-sults showed that crude acetone extract for W. prolifica better than the haxane extract and more efficient on negative gram bacteria than positive gram bacteria. The results of the agar well diffusion method evaluated that W. Prolifica acetone extract has the highest antibacterial activities against Streptococcus sp., S. aureus and A.niger with an inhibition zone of (20) mm, and the inhibition diam-eter to other bacteria and fungi were between(15-10) mm.While tube method showed that the ace-tone extract exhibited the highest inhibition against A.niger and less inhibiting to C. albicans. Purifica-tion of the acetone extracts was made by silica gel column chromatography, and among the five groups extracts, Group 2 (Benzene 50ml) was selected and analyzed by GC-MS. The presence of main components identified in the extract as alcohols, acids, monoterpene eucalyptol, hydrocarbons (unidecane) aromaticslike, Para- Xylene and 1,2,3 trimethyl benzene, Phytol, n-Hexadecanoic acid, etc. These purified active compounds take part into broad horizons in the fields of biotechnology and pharmacy.

Keywords


Antimicrobial activity, Active compound, Cyanobacteria, Westiellopsis prolifica.

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References


Alwathnani H., and Perveen, K. An-tibacterial activity and morphological changes in human pathogenic bacte-ria caused by Chlorella vulgaris ex-tracts. Biomedical Research, 28 (4): 2017, pp.1610-1614.

Salem, O., Hoballah, E., Safia, M. and Hanna, N. Antimicrobial activity of microalgal extracts with special em-phasize on Nostoc sp. Life Science Journal, 11(12): 2014, pp.752-758.

Syed,S.; Arasu, A. and Ponnus I., The Uses of Chlorella Vulgaris as Antimi-crobial Agent and as Diet: the Pres-ence of Bio-active Compounds which caters the Vitamins, Minerals in Gen-eral. International Journal of Bio-Science and Bio-Technology, Vol.7, No.1: 2015, pp.185-190.

Pulz, O. and Gross, W. Valuable products from biotechnology of micro-algae. Appl. Microbiol. Biotechnol, 65(6): 2004, 635-48.

Thamilvanan, D., Karthikeyan, D., Muthukumaran, M. and Balakumar, B. Antibacterial activity of selected micro-algal members of chlorophyceae. World J. of Pharmacy and Pharmaceutical Sci-ences. 5 (10): 2016, pp. 718-729.

Al-Wathnani H.; Ismet A.; Tahmaz, R.; Al-Dayel T. H. and Bakir M. Bio-activity of natural compounds isolat-ed from cyanobacteria and green al-gae against human pathogenic bacte-ria and yeast. Journal of Medicinal Plants Research, Vol. 6(18): 2012, pp. 3425-3433.

Desikachary, T.V. Cyanophyta. Indi-an Council of Agricultural Research, New Delhi, 1959.

Rippka, R.; Deruelles, J.; Waterbury, J.; Herdman, M. and Stanier, R. Ge-neric Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. J. Gen. Microbiol., 111(1): 1979, pp:1-61.

Elnabris, K.; Elmanama, A. and Chi-hadeh, W. Antibacterial activity of four marine seaweeds collected from the coast of Gaza Strip, Palestine. Mesopot. J. Mar. Sci., 28(1): 2013, pp.81 – 92.

Basniwal, K. and Purshotam K. Bio-fuel Production from Microalgae for Energy Applications. J. Algal Bio-mass Utln. 5(4): 2014, pp.50-54

Patra, J., Patra, A., Mahapatra, N., Thatoi, N., Das, S., Sahu, R. and Swain, G. Antimicrobial activity of organic solvent extracts of three ma-rine macroalgae from Chilika Lake, Orissa, India. Malaysian Journal of Microbiology,; 5(2): 2009, pp.128-131.

Duygu, D.; Udoh, A.; Ozer, T.; Ak-bulut, A.; Erkaya, I.; Yildiz, K. and Guler, D. Fourier transform infrared (FTIR) spectroscopy for identification of Chlorella vulgaris Beijerinck 1890 and Scenedesmus obliquus (Turpin) Kützing 1833. African Journal of Bio-technology, Vol. 11(16): 2012, pp. 3817-3824.

Lalitha, R., and Palani, S. Phyto-chemical analysis of Scinaia ben-galica by GC-MS. International Journal of ChemTech Research, Vol.10 No.1: 2017, pp. 134-138.

SAS. Statistical Analysis System, Us-er's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA, 2012.

Alica, B.; Lenka, B. and Kristína, G. Charactrisation of polysaccharides and lipids from selected green algae species by FTIR-ATR spectroscopy. Slovak university of technology in Bratislava. 23(36): 2015, pp.97-102

Al-Hashimi, A.; Shahrazad, N.; Al-Rrubaai, G. and Roaa, F. The effect of microalgae extraction on bacterial species isolated from seminal fluid of sexually- active males in Baghdad. Journal of Genetic and Environmen-tal Resources Conservation, 4(2): 2016, pp.171-177.

Digamber, R. Antibacterial Activity of Fresh Water Cyanobacteria. J. Algal Biomass Utln. 6 (3): 2015, pp.60- 64.

Entesar, A. and Ahmed, A. Antimi-crobial Activity of Microalgal Ex-tracts Isolated From Baharia Oasis, Egypt. Global Advanced Research Journal of Microbiology, 5(3): 2016, pp. 033-041.

Naveen, B.; Irshad, M.; Moshahid A., and Tasneem F., Antimicrobial and Cytotoxic Activi-ties of. Cyanobacteria. International Journal of Innovative Research in Science, Engineering and Technology. 2 ( 9): 2013, pp.4328-4343.

Sirikul T.; Kun S.and Siriporn S. An-tibacterial activity of crude extracts of cyanobacteria Phormidium and Mi-crocoleus species. African Journal of Microbiology Research. Vol. 6(10): 2012, pp. 2574-2579.

Al.Rubiee, H. and Dauu, S. Influence the effectiveness of compounds pro-duced from algae Oscllatoria princeps against bacteria and detected of some fatty acids. Iraqi Journal of Science, 54 (2): 2013, pp.266-273.

Shannon, E., and Abu-Ghannam, N. Antibacterial Derivatives of Marine Algae: An Overview of Pharmacologi-cal Mechanisms and Applications. Mar. Drugs, 14( 81): 2016, pp.1-23.

De Morais, M.; Vaz, B.; de Morais, E. and Costa, J. Biologically active me-tabolites synthesized bymicroalgae. BioMed research international. ID 835761, 2015, 15 pages.

Neveen, A., and Ibraheem, B. Ibra-heem, M. Antibiotic activity of two Anabaena species against four fish pathogenic Aeromonas species. Afri-can Journal of Biotechnology, Vol. 7 (15): 2008.

Ratnam K V, Raju RRV. In vitro an-timicrobial screening of the fruit ex-tracts of two Syzygium species (Myr-taceae) Advan Biol Res. 2008; 2(1-2):17–20

María José Pérez, Elena Falqué, and Herminia Domínguez Antimicrobial Action of Compounds from Marine Seaweed. Journal of Marins drugs .14(3) :2016, pp.1-38




DOI: http://dx.doi.org/10.23851/mjs.v29i3.621

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