Detection of Flagellin Gene of Enteric Isolates of Salmonella typhi Using PCR in Baghdad Governorate

saba saadoon khazaal

Abstract


The study was conducted to study the virulence gene (Flagellin gene) of Salmonella typhi. Stool samples were collected from adults patients, their age ranging from (15 – 60 ) years of the both sexes who suffering from diarrhea and visited AL- Yrmouk Teaching Hospital in Baghdad Governorate for the period between March to September 2017. The samples were transferred in cold boxes to the laboratory. In order to confirm the diagnosis, the polymerase chain reaction (PCR) was tested to diagnose the Flagellin gene responsible for the virulence of the bacteria. We conclude from the present study that flagellin gene in S. typhi is responsible for pathogenicity in this bacteria.

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DOI: http://dx.doi.org/10.23851/mjs.v30i3.329

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