Anti-Ovarian Vessels Antibodies in P. aeruginosa Rabbit Hyper Immune Sera, an Immunohistochemical Study

Noor Al-Huda A. Saeed

Abstract


Cyclical angiogenesis in the ovary is a unique process supporting normal folliculogenesis as well as lute genesis. In this report we investigated the reactivity of rabbit anti pseudomonas aeruginosa antisera with ovarian blood vessels.
Tissues stained with anti-sera were immunohistochemically visualized using biotinylated anti rabbit immunoglobulin and peroxidase conjugated streptavidin. Positive staining sites depend on anti-stain type, however, staining was observed in endothelial cell and tunica adventitia in most cases.On the other hand, corpus luteum blood vessels showed a positive staining pattern as well.
We conclude from this study that a peculiar staining pattern was seen in ovarian blood vessels stained with rabbit anti-pseudomonas aeruginosa hyper immune sera, the importance of this reactivity need further investigation.

Keywords


Anti-ovarian vessels antibodies, pseudomonas aeruginosa, immunohistochemical

Full Text:

PDF

References


FraserH. M. and Duncan W.C.: SRB reproduction and fertility development award lecture 2008. Regulation and manipulation of angiogenesis in teovary and endometriam. Reproduction, Fertility and Development. 21, 377-392. (doi: 10.1071/RDO 8272, 2009. Geva E. and Jaffe R.B.: Role of vascular endothelial growth factor in ovarian physiology and pathology. Fertl.Sterl.74: 429-438, 2000. CrossRef

Mattioli M., Barboni B., Turriani M., Galeati G., Zannoi A.: Follicle activation involves vascular endothelial growth factor production and increased blood vessel extension. Biol. Repod. 65: 1014-1019, 2001. CrossRef

Tamanini C. and De Ambrogi M.: Angiogenesis in developing follicle and corpus luteum. Reproduction in Domestic Animals.39 (4): 206-216, 2004. CrossRef

Berardinelli P., Russo V., Martelli A., Nardioncchi D., Di Giacinto O.: Colocalization of DNA Fragmentation and Caspase-3 activation during atresia in pig antral follicles. Anat. Istol. Embryo.33: 23-27, 2004. CrossRef

Plend J.: Angiogenesis and vascular regression in the ovary. Anat. Histol. Embroyl. 29: 257-266, 2000. CrossRef

Folkman J. and Klagsbrun M.: angiogenesis factors science 233.442-447, 1987.

Abulafia O. and Sherer D.M. : Angiogenesis of te ovary. Am. J. Obstet. Gynec. 182, 240-246, 2000. CrossRef

Ferrara N.: VEGF and the quest for tumor angiogenesis factors. Nat. Rev. Cancer. 2: 795-803, 2002. CrossRef

Shibuya M., Luo J. C., Toyoda M., Yamaguci S.: Involvement of VEGF and its receptors in ascites tumor formation. Cancer. Chemother. Pharmacol. 43: 72-77, 1999. CrossRef

Robinson R. S, Wood K. J, Hammond A. J, Laird M., unter M. G.: Angiogenesis and vascular function in the ovary. Reprod. 138: 869-881, 2009. CrossRef

Duncan N. H, Hinton N. A, Penner J. L, Duncan I. B.R.: Preparation of typing antisera specific for O antigens of pseudomonas aeruginosa. J. Clin. Microbiol. 4: 124-128, 1976.

Guesdon JL, Ternynck T, Avrameas S.: The use of avidin biotin interaction in immunoenzymatic techniques. J Histochem Cytochem; 27 (8): 1131-9, 1979. CrossRef

Giorno R.: Acomparison of two immunoperoxidase staining methods based on the avidin-biotin interaction Diag Immunol; 2 (3): 161-6, 1984.

Modlich U, Kaup FJ, Augustin HG.: Cyclic angiogenesis and blood vessel regression in the ovary: blood vessel regression during luteolysis involves endothelial cell detachment and vessel occlusion.;74 (4): 771-80, 1996.

Fraser HM.: Regulation of the ovarian follicular vasculature. Reprod Biol Endocrinol.4: 8-22, 2006. CrossRef

Mattioli M., Barboni B., Turriani M., Galeati G., Zannoni A., Castellani G., Berardinelli P., Scapolo PA.: Folicle activation involves vascular endothelial growth factor production and increased blood vessel extension. Biol Repord. 65: 1014-1019, 2001. CrossRef

Gavalas N. G, Liontos M., Trachana S.P, Bagratuni T, Arapinis C, Liacos C, Dimopoulos M. A, Bamias A.: Angiogenesis-related pathways in the pathogenesis of ovarian cancer. Int. J. Mol. Sci.14: 15885-15909, 2013. CrossRef

Augustin HG.: Vascular morphogenesis in the ovary.Baillieres best pract res clin obstet Gynaecol;14 (6): 867-82, 2000. CrossRef

Burger R.A.: Overview of anti-angiogenic agents in development for ovarian cancer. Gynecol. Oncol.121: 230-238, 2011. CrossRef

Burger R. A, Still M. W, Monk B. J, Greer B. E, Sorosky J. I: Phase II-trial of beracizumab in persistent or recurrent epithelial ovarian cancer or primary peritoneal cancer. A Gynecologic Oncology Group study. J. Clin. Oncol.25: 5165-5171, 2007. CrossRef

Mauro A., Martelli A., Berardinelli P., Ruso V., Bernabo N., Di Giacinto O., Mattioli M., Barboni B.: Effect of antiprogesterone RU486 on VEGF expression and blood vessl remodeling on ovarian follicle before ovulation-PLOSONE qie 95910. Doi: 10.1371, 2013.

Smith-McCune K. K, Weidner N.: Demonstration and characterization of the angiogenic properities of cervical dysplasia. Cancer. Res. 54: 800-804, 1994.




DOI: http://dx.doi.org/10.23851/mjs.v28i1.313

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Al-Mustansiriyah Journal of Science

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.


Copyright (c) 2018 by Al-Mustansiriyah Journal of Science
ISSN: 1814-635X (Print), ISSN: 2521-3520 (online)