Antimicrobial Effects of Mentha Pulegium Extract against Staphyloccocus Aureus Bacteria

Nehia hussein, Zainab Nabeel


    The effects of aqueous and alcoholic of extracts Mentha pulegium leaves were studied on the growth of strain Staphylococcus aureus that isolated from burns. The Phytochemical analysis of The aqueous and alcoholic extracts of Mentha pulegium leaves show the presence of bioactive constituents like comarins, steroids, tannins, glycosides, saponins, alkaloids, flavonoids, phenols  and essential oil. the ethanolic extract show an inhibition zone larger than aqueous extract.Results show that the ethanolic extract inhibits bacterial growth with inhibition zone(30.33mm) but the aqueous extract give an inhibition zone(8mm). In this study, minimum inhibitory concentration (MIC) was detected and the result varies according to the type of extract.


Mentha pulegium,Ethanolic extract,Aqueous extract ,MIC.

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Brooks, G.F.; Butel, J.S. and Morse, S.A (2004). Jawetz, Melnick Adelbergs Medical Microbiology, (23rd)ed. McGraw– Hill.

Turkyilmaz, S. and Kaya, O. (2006). Determination of some virulence factors in staphylococcus spp. Isolated from various clinical samples. Turkish Journal of Veterinary and Animal Sciences, 30: 127- 132.

Dubin, G. (2002). Extracellular Proteases of Staphylococcus spp. Biological Chemistry, 383: 1075-1086.

Midorikawa, K.; Ouhara, K.; Komatsuzawa, H.; Kawai, T.; Yamada, S.; Fugiwava, T.; Yamazaki, K.; Sayama, K.; Taubman, M.A.; Kurihara, H. ; Hashimoto, K. and Sugai, M. (2003). Staphylococcus aureus susceptibility to innate antimicrobial peptides, ß –defenses and CAP18, expressed by human kerationcytes. Infection and Immunity, 71(7): 3730 - 3739.

Olufunmiso, O.O. and Anthony, J.A. (2012). In Vitro Antibacterial and Time-Kill Assessment of Crude Methanolic Stem Bark Extract of Acacia mearnsii De Wild against Bacteria in Shigellosis. Molecules 17, 2103-2118.

Miriam ,L.G.(2001). Technical information herbamel .Honey with mint , Propois and peppermint .CRF/ SP:23,939.

Watt, M. (2002), Correcting the gross errors in aromatherapy teaching, Internet news group.

الشحات ، نصر ابو زيد . (1984) . النباتات العطرية ومنتجاتها الزرعية والدوائية . دار العربية للنشر والتوزيع .

المنظمة العربية للتنمية الزراعية . (1988) . النباتات الطبية والعطرية السامة في الوطن العربي ، الخرطوم .

Boskabady, M.H.; Rakhshandah, H.; Afiat, M.; Aelami, Z. and Amiri, S. (2002). Antitissue effect of plantago lanceolata in guinea pigs. Iranian Journal Medicine Science, vol. 31(3): p. 143-146.

El-Mahmood, A. M.; Doughari, J. H. and Kiman H. S.(2010). In vitro antimicrobial activity of crude leaf and stem bark extracts of Gmelina arborea (Roxb) against some pathogenic species of Enterobacteriaceae. African Journal of Pharmacy and Pharmacology Vol. 4(6). pp. 355-361.

A. Šovčíková, M. Mikulášová, K. Horáková, L'. Floch; Antibacterial and Mutagenic activities of new isothiocyanate derivatives. Folia Microbiol (Praha), 46:113–117.

Jawad, A. Ethnological studies in assessing the anti-aggressive effects of some Iraqi medical plants in laboratory mice. (1997). PhD.Thesis, Coll. Edu. Univ. Basrah.

Du Mee C. Vitex agnus castus. Aust J. Med. Herbalism. (1993); 5:(63-65).

Gayon, P. Plant phenolics. 11th (ed.), Oliver and Boye, Edinburge. (1972). p. 254.

Newall C.A, Anderson L.A, Phillipson J.D. Herbal medicines: a guide for health-care professionals. London: Pharmaceutical Press. (1996). PP. 296.

Haddad D. The chemistry of vegetable drug. Part 2, Cairo Univ. press, Cairo, Egypt. (1965). pp. 127.

Tyler V, Braady L& Robber J. Pharmacology. (19th ed.) , Lea. And Febiger, (1988). USA.

McGuffin M, Hobbs C, Upton R& Goldberg A. American Herbal Products Association's Botanical Safety Handbook. Boca Raton. New York: CRC Press. (1997). pp.231.

Mahmoud M. Jamiel.(2008): Chemistry Of Medicinal Plants.

Atlas, R.M.; Brown, A.E. and Parks, L.C. (1995). Exprimental Microbiology Laboratory Manual. Mc Graw. Hill Companies Mos -by Company. St. Louis.

Francis, G.; Kerem, Z.; Makkar, H.P.; Becker, K. (2002). The biological action of saponins in animal systems: A review. Br J Nutr., 88:587–605.

Atlas, R.M.; Brown, A.E. and Parks, L.C. (1995). Exprimental Microbiology Laboratory Manual. Mc Graw. Hill Companies Mos -by Company. St. Louis.

AL-Ani ,A.J., Nadir ,M.T .and Al-Khazraji .N.R.(1996). The antimicrobial activity of volatile oil isolated from some Iraq plants .J. AL-Anbar University .11(1): 82-86.

الزبيدي, زهير نجيب وبابان, هدى عبد الكريم وفليح وفـارس كـاظم (1996). دليـل العـلاج بالأعشاب الطبية العراقي .شركة آب للطباعة الفنية المحدودة / بغداد .



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