Effect of Water and Alcohol Extracts of Myrtus communis and Camellia sinensis in the Growth and Efficacy of Pseudomonas aeruginosa Bacteria and Escherichia coli Bacteria

Raad Abdul Hadi Nyif

Abstract


Abstract

The aim of this study was to know the effect of water and alcohol extracts of two medicinal plants, Myrtle and Green Tea, in inhibiting the growth of two types of bacteria: Pseudomonas aeruginosa and Escherichia coli.  The study also tested the resistance of bacterial isolates to Tetracyclin antibiotic. The pH of leaves of alias and green tea was measured.

Gradient concentrations of each plant extract were used to determine the concentrations in which the bacteria showed their growth, which gave a vitality almost equal to the control group.  The study concluded that:

The water extract of Myrtle leaf and green tea was found to be effective in reducing the growth of bacteria at concentrations (10, 20, 30) and (10, 15, 20%) compared with the alcoholic extract of both plants which was less effective.

The antibiotic showed a clear inhibitory effect on E.coli at concentration (0.1) ml while P. aeruginosa showed clear antibody resistance.


Keywords


Myrtus communis, Camellia sinensis, Pseudomonas aeruginosa and Escherichia coli

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References


Ahmed, S.O.(2012) Study the effect of aqueous and alcoholic extracts of some spices on some pathogenic bacteria in different media. Journal of Tikrit University of Agriculture sciences 12. (1), 1-11.

El- Gali, Z.l., Mohamed, N.A. and larbod, A.A. (2012) variability in virulence of five isolates of Botrytis cinerea on three onion eultivars. 3, 3, (11), 43 – 51.

Mahboubi , A., Kamalinejad. M., Shalriri, M., Karbasi, Z., Jafariazar, Z. and Asgharian, R. (2012) Evaluation of antibacterial activity of three Iranian Medicinal plants. African Journal of microbiological researches 6, (a), 2048 – 2052.

Hosseini Jazani , N.; shahabi m sh.; Abdi Ali, A.& Zart. oshti , M. (2007). antibacterial effects of water soluble green tea extracts on multi – antibiotic of resistant isolates of Acinetobacter SP. Pakistan . J.Biol. SCi., 10 (a) : 1477 -1480.

Taoubi, K.; Traboulsi, A.F.; EL- Haj, S.; Bessiere .J,.M and Rammal, S. (2002) . insecticidal properties of essential plant oils against the Mosquito Culex pipiene molestus (Diptera: culicidae). pest manage. Sci., 58 (5): 491 – 495.

Atlas, R.M. (1995). principles of microbiology. Mosby-Year book, inc., St. Louis: 888pp.

Simonetti, G.; simonetti, N.; A.; Increased Microb-ieidal activity of green tea (camellia sinensis) in combination with butylated hydroxyaniso J.chemother. 2004 Apr. 16 (2): 122-7.

Xian.Z .L. Zhang, L.; Srikumar, R. & Poole, K.(1998). B- Lactamase inhibitors are substrate for the multidring efflux pwnp of pseudomonas arngiuosa . Antimicrobial., Agents chemoother 42 (2): 399-403.

Levinson, W. & Jawetz, E. (2000). Medical microbiology & immunology (examination & board review) , 6th Singapore , New Delhi : 582 PP.

Medica, M (2004). principia pharmacopeia of essential oils. www.mendocinosdap.com.

Sato, J.; Goto, K.; Nanjo. f.; Kowai, S. & Murata, K. (2000). Antimicrobial activity of plant extracts against Arthrinium sacchari and chactomium funicola.J. Biosci. Bioeug, 90 (4): 442-446.

Deshmukh, S. D. & Borle , M.N. (1975). studies on the insectidal properties of indigenous plant products Indiah. J. Ent ., 37 (1): 11 – 18.




DOI: http://dx.doi.org/10.23851/mjs.v29i1.149

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